Near-Infrared Spectroscopic Analysis of Total Phenolic Content and Antioxidant Activity of Berry Fruits

This study evaluates the feasibility of using near-infrared (NIR) spectroscopy as a rapid and environmentally friendly technique for validation and prediction of the total phenolic content (TPC) and antioxidant activity (AOA) indices (as 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging, inhib...

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Main Authors: Jasenka Gajdoš Kljusurić, Kiril Mihalev, Ivana Bečić, Ivana Polović, Mariya Georgieva, Senka Djaković, Želimir Kurtanjek
Format: Article
Language:English
Published: University of Zagreb Faculty of Food Technology and Biotechnology 2016-01-01
Series:Food Technology and Biotechnology
Subjects:
Online Access:https://hrcak.srce.hr/file/236625
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author Jasenka Gajdoš Kljusurić
Kiril Mihalev
Ivana Bečić
Ivana Polović
Mariya Georgieva
Senka Djaković
Želimir Kurtanjek
author_facet Jasenka Gajdoš Kljusurić
Kiril Mihalev
Ivana Bečić
Ivana Polović
Mariya Georgieva
Senka Djaković
Želimir Kurtanjek
author_sort Jasenka Gajdoš Kljusurić
collection DOAJ
description This study evaluates the feasibility of using near-infrared (NIR) spectroscopy as a rapid and environmentally friendly technique for validation and prediction of the total phenolic content (TPC) and antioxidant activity (AOA) indices (as 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging, inhibition time (IT) of the Briggs-Rauscher oscillating reaction, and relative antioxidant capacity (RAC)) of berry fruit extracts. The analysed berry samples originated from Croatia (blackberries, wild blueberries, raspberries, red currants and strawberries) and Bulgaria (wild blueberries, raspberries and strawberries). Principal component analysis and partial least squares (PLS) regression were used from the set of chemometric tools in distinguishing and validating the measured berry fruit extract. ANOVA and PCA showed no significant impact of the origin and freshness of the samples. PLS models were developed to validate the relationship of NIR spectra with TPC and AOA of berry fruits. Representativeness of the models was expressed with the R2 and the ratio of performance to deviation. Calculated R2 values were above 0.84 and the ratio of performance to deviation was between 1.8 and 3.1, indicating adequacy of the PLS models.
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spelling doaj.art-01c148f3cc9c445d91a81591b6063fa32024-04-15T13:51:39ZengUniversity of Zagreb Faculty of Food Technology and BiotechnologyFood Technology and Biotechnology1330-98621334-26062016-01-0154223624210.17113/ftb.54.02.16.4095Near-Infrared Spectroscopic Analysis of Total Phenolic Content and Antioxidant Activity of Berry FruitsJasenka Gajdoš Kljusurić0Kiril Mihalev1Ivana Bečić2Ivana Polović3Mariya Georgieva4Senka Djaković5Želimir Kurtanjek6Faculty of Food Technology and Biotechnology, University of Zagreb, Zagreb, CroatiaUniversity of Food Technologies, Department of Food Preservation and Refrigeration Technology, Plovdiv, BulgariaFaculty of Food Technology and Biotechnology, University of Zagreb, Zagreb, CroatiaFaculty of Food Technology and Biotechnology, University of Zagreb, Zagreb, CroatiaUniversity of Food Technologies, Department of Food Preservation and Refrigeration Technology, Plovdiv, BulgariaFaculty of Food Technology and Biotechnology, University of Zagreb, Zagreb, CroatiaFaculty of Food Technology and Biotechnology, University of Zagreb, Zagreb, CroatiaThis study evaluates the feasibility of using near-infrared (NIR) spectroscopy as a rapid and environmentally friendly technique for validation and prediction of the total phenolic content (TPC) and antioxidant activity (AOA) indices (as 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging, inhibition time (IT) of the Briggs-Rauscher oscillating reaction, and relative antioxidant capacity (RAC)) of berry fruit extracts. The analysed berry samples originated from Croatia (blackberries, wild blueberries, raspberries, red currants and strawberries) and Bulgaria (wild blueberries, raspberries and strawberries). Principal component analysis and partial least squares (PLS) regression were used from the set of chemometric tools in distinguishing and validating the measured berry fruit extract. ANOVA and PCA showed no significant impact of the origin and freshness of the samples. PLS models were developed to validate the relationship of NIR spectra with TPC and AOA of berry fruits. Representativeness of the models was expressed with the R2 and the ratio of performance to deviation. Calculated R2 values were above 0.84 and the ratio of performance to deviation was between 1.8 and 3.1, indicating adequacy of the PLS models.https://hrcak.srce.hr/file/236625berry fruitsantioxidant activityNIR spectroscopyPLS modelstotal phenolic content
spellingShingle Jasenka Gajdoš Kljusurić
Kiril Mihalev
Ivana Bečić
Ivana Polović
Mariya Georgieva
Senka Djaković
Želimir Kurtanjek
Near-Infrared Spectroscopic Analysis of Total Phenolic Content and Antioxidant Activity of Berry Fruits
Food Technology and Biotechnology
berry fruits
antioxidant activity
NIR spectroscopy
PLS models
total phenolic content
title Near-Infrared Spectroscopic Analysis of Total Phenolic Content and Antioxidant Activity of Berry Fruits
title_full Near-Infrared Spectroscopic Analysis of Total Phenolic Content and Antioxidant Activity of Berry Fruits
title_fullStr Near-Infrared Spectroscopic Analysis of Total Phenolic Content and Antioxidant Activity of Berry Fruits
title_full_unstemmed Near-Infrared Spectroscopic Analysis of Total Phenolic Content and Antioxidant Activity of Berry Fruits
title_short Near-Infrared Spectroscopic Analysis of Total Phenolic Content and Antioxidant Activity of Berry Fruits
title_sort near infrared spectroscopic analysis of total phenolic content and antioxidant activity of berry fruits
topic berry fruits
antioxidant activity
NIR spectroscopy
PLS models
total phenolic content
url https://hrcak.srce.hr/file/236625
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