Rapid Detection of Campylobacter jejuni by Polymerase Chain Reaction and Evaluation of its Sensitivity and Specificity
Introduction: Campylobacter jejuni is one of the most common causes of food poising in humans. Rapid and specific detection of these bacteria has an important role in diagnosis and treatment of infection. The aim of this study was to design a specific PCR for the detection of Campylobacter jejuni....
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| Format: | Article |
| Language: | fas |
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Hamadan University of Medical Sciences
2017-06-01
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| Series: | پزشکی بالینی ابن سینا |
| Subjects: | |
| Online Access: | http://sjh.umsha.ac.ir/article-1-1458-en.html |
| _version_ | 1828841667403186176 |
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| author | A Razei R Sorouri H Aghamollaei SL Mousavi |
| author_facet | A Razei R Sorouri H Aghamollaei SL Mousavi |
| author_sort | A Razei |
| collection | DOAJ |
| description | Introduction: Campylobacter jejuni is one of the most common causes of food poising in humans. Rapid and specific detection of these bacteria has an important role in diagnosis and treatment of infection. The aim of this study was to design a specific PCR for the detection of Campylobacter jejuni.
Methods: In this experimental study, oxidoreductase gene from the Campylobacter jejuni was selected for rapid and specific detection. For this purpose, specific primers were designed and charecterized by bioinformatics software. Bacterial genome was extracted by phenol-chloroform method and PCR was optimized to obtain a specific product. Specificity of the designed reaction was investigated using six bacterial species. The sensitivity of the PCR reaction was calculated by the serial dilutions method.
Results: The designed primer was specific to oxidoreductase gene of Campylobacter jejuni and after optimization, a unique 167-bp band was amplified. This primer was specific to Campylobacter jejuni and did not show any cross reaction with other bacterial genomes. The detection limit of this reaction was 5 pg of genomic DNA.
Conclusions: The optimized PCR used in this study was a rapid, sensitive, and specific method for detection of Campylobacter jejuni. For confirmation of this method, detection of C. jejuni from food samples is proposed. |
| first_indexed | 2024-12-12T20:08:01Z |
| format | Article |
| id | doaj.art-01c284570cf44b5f836b612a5211502f |
| institution | Directory Open Access Journal |
| issn | 2588-722X 2588-7238 |
| language | fas |
| last_indexed | 2024-12-12T20:08:01Z |
| publishDate | 2017-06-01 |
| publisher | Hamadan University of Medical Sciences |
| record_format | Article |
| series | پزشکی بالینی ابن سینا |
| spelling | doaj.art-01c284570cf44b5f836b612a5211502f2022-12-22T00:13:35ZfasHamadan University of Medical Sciencesپزشکی بالینی ابن سینا2588-722X2588-72382017-06-012415662Rapid Detection of Campylobacter jejuni by Polymerase Chain Reaction and Evaluation of its Sensitivity and SpecificityA Razei0R Sorouri1H Aghamollaei2SL Mousavi3 Bqiyatallah University of Medical Sciences Bqiyatallah University of Medical Sciences Bqiyatallah University of Medical Sciences Shahed University Introduction: Campylobacter jejuni is one of the most common causes of food poising in humans. Rapid and specific detection of these bacteria has an important role in diagnosis and treatment of infection. The aim of this study was to design a specific PCR for the detection of Campylobacter jejuni. Methods: In this experimental study, oxidoreductase gene from the Campylobacter jejuni was selected for rapid and specific detection. For this purpose, specific primers were designed and charecterized by bioinformatics software. Bacterial genome was extracted by phenol-chloroform method and PCR was optimized to obtain a specific product. Specificity of the designed reaction was investigated using six bacterial species. The sensitivity of the PCR reaction was calculated by the serial dilutions method. Results: The designed primer was specific to oxidoreductase gene of Campylobacter jejuni and after optimization, a unique 167-bp band was amplified. This primer was specific to Campylobacter jejuni and did not show any cross reaction with other bacterial genomes. The detection limit of this reaction was 5 pg of genomic DNA. Conclusions: The optimized PCR used in this study was a rapid, sensitive, and specific method for detection of Campylobacter jejuni. For confirmation of this method, detection of C. jejuni from food samples is proposed.http://sjh.umsha.ac.ir/article-1-1458-en.htmlcampylobacter jejuni- diagnosisoxidoreductasepolymerase chain reaction |
| spellingShingle | A Razei R Sorouri H Aghamollaei SL Mousavi Rapid Detection of Campylobacter jejuni by Polymerase Chain Reaction and Evaluation of its Sensitivity and Specificity پزشکی بالینی ابن سینا campylobacter jejuni- diagnosis oxidoreductase polymerase chain reaction |
| title | Rapid Detection of Campylobacter jejuni by Polymerase Chain Reaction and Evaluation of its Sensitivity and Specificity |
| title_full | Rapid Detection of Campylobacter jejuni by Polymerase Chain Reaction and Evaluation of its Sensitivity and Specificity |
| title_fullStr | Rapid Detection of Campylobacter jejuni by Polymerase Chain Reaction and Evaluation of its Sensitivity and Specificity |
| title_full_unstemmed | Rapid Detection of Campylobacter jejuni by Polymerase Chain Reaction and Evaluation of its Sensitivity and Specificity |
| title_short | Rapid Detection of Campylobacter jejuni by Polymerase Chain Reaction and Evaluation of its Sensitivity and Specificity |
| title_sort | rapid detection of campylobacter jejuni by polymerase chain reaction and evaluation of its sensitivity and specificity |
| topic | campylobacter jejuni- diagnosis oxidoreductase polymerase chain reaction |
| url | http://sjh.umsha.ac.ir/article-1-1458-en.html |
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