Kallikrein-11, in Association with Coiled-Coil Domain Containing 25, as a Potential Prognostic Marker for Cholangiocarcinoma with Lymph Node Metastasis
Cholangiocarcinoma (CCA) is a malignancy arising from cholangiocytes. Currently, the treatment and prognosis for CCA are mostly poor. Recently, we have reported that coiled-coil domain containing 25 (CCDC25) protein level in the sera may be a diagnostic marker for CCA. Subsequently, we identified th...
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MDPI AG
2021-05-01
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| author | Saeranee Siriphak Ravinnipa Chanakankun Tanakorn Proungvitaya Sittiruk Roytrakul Doungdean Tummanatsakun Wunchana Seubwai Molin Wongwattanakul Siriporn Proungvitaya |
| author_facet | Saeranee Siriphak Ravinnipa Chanakankun Tanakorn Proungvitaya Sittiruk Roytrakul Doungdean Tummanatsakun Wunchana Seubwai Molin Wongwattanakul Siriporn Proungvitaya |
| author_sort | Saeranee Siriphak |
| collection | DOAJ |
| description | Cholangiocarcinoma (CCA) is a malignancy arising from cholangiocytes. Currently, the treatment and prognosis for CCA are mostly poor. Recently, we have reported that coiled-coil domain containing 25 (CCDC25) protein level in the sera may be a diagnostic marker for CCA. Subsequently, we identified three binding proteins of CCDC25 and found that kallikrein-11 (KLK11) expression was highest among those binding proteins. In this study, we investigated CCDC25 and KLK11 expression in CCA and adjacent normal tissues (<i>n</i> = 18) using immunohistochemistry. The results demonstrated that the expressions of CCDC25 and KLK11 in CCA tissues were both significantly higher than the adjacent tissues (<i>p</i> < 0.001 and <i>p</i> = 0.001, respectively). Then, using GEPIA bioinformatics analysis, KLK11 mRNA was significantly overexpressed in CCA tumor tissues compared with normal tissues (<i>p</i> < 0.05). Moreover, CCDC25 expression was positively correlated with KLK11 expression in CCA with lymph node metastasis (<i>p</i> = 0.028, r = 0.593). An analysis for the interaction of KLK11 with CCDC25 and other proteins, using STRING version 11.0, revealed that CCDC25 and KLK11 correlated with metastasis-related proteins. In addition, Kaplan-Meier survival curve analysis revealed that a high expression of KLK11 was associated with the poor prognosis of CCA. In conclusion, KLK11 is, as a binding protein for CCDC25, possibly involved in the metastatic process of CCA. KLK11 may be used as a prognostic marker for CCA. |
| first_indexed | 2024-03-10T11:07:57Z |
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| language | English |
| last_indexed | 2024-03-10T11:07:57Z |
| publishDate | 2021-05-01 |
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| series | Molecules |
| spelling | doaj.art-01c657af485344c0abbec292b350973e2023-11-21T20:56:58ZengMDPI AGMolecules1420-30492021-05-012611310510.3390/molecules26113105Kallikrein-11, in Association with Coiled-Coil Domain Containing 25, as a Potential Prognostic Marker for Cholangiocarcinoma with Lymph Node MetastasisSaeranee Siriphak0Ravinnipa Chanakankun1Tanakorn Proungvitaya2Sittiruk Roytrakul3Doungdean Tummanatsakun4Wunchana Seubwai5Molin Wongwattanakul6Siriporn Proungvitaya7Centre of Research and Development of Medical Diagnostic Laboratories, Faculty of Associated Medical Sciences, KhonKaen University, Khon Kaen 40002, ThailandCentre of Research and Development of Medical Diagnostic Laboratories, Faculty of Associated Medical Sciences, KhonKaen University, Khon Kaen 40002, ThailandCentre of Research and Development of Medical Diagnostic Laboratories, Faculty of Associated Medical Sciences, KhonKaen University, Khon Kaen 40002, ThailandNational Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Pathum Thani 12120, ThailandCentre of Research and Development of Medical Diagnostic Laboratories, Faculty of Associated Medical Sciences, KhonKaen University, Khon Kaen 40002, ThailandCholangiocarcinoma Research Institute, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, ThailandCentre of Research and Development of Medical Diagnostic Laboratories, Faculty of Associated Medical Sciences, KhonKaen University, Khon Kaen 40002, ThailandCentre of Research and Development of Medical Diagnostic Laboratories, Faculty of Associated Medical Sciences, KhonKaen University, Khon Kaen 40002, ThailandCholangiocarcinoma (CCA) is a malignancy arising from cholangiocytes. Currently, the treatment and prognosis for CCA are mostly poor. Recently, we have reported that coiled-coil domain containing 25 (CCDC25) protein level in the sera may be a diagnostic marker for CCA. Subsequently, we identified three binding proteins of CCDC25 and found that kallikrein-11 (KLK11) expression was highest among those binding proteins. In this study, we investigated CCDC25 and KLK11 expression in CCA and adjacent normal tissues (<i>n</i> = 18) using immunohistochemistry. The results demonstrated that the expressions of CCDC25 and KLK11 in CCA tissues were both significantly higher than the adjacent tissues (<i>p</i> < 0.001 and <i>p</i> = 0.001, respectively). Then, using GEPIA bioinformatics analysis, KLK11 mRNA was significantly overexpressed in CCA tumor tissues compared with normal tissues (<i>p</i> < 0.05). Moreover, CCDC25 expression was positively correlated with KLK11 expression in CCA with lymph node metastasis (<i>p</i> = 0.028, r = 0.593). An analysis for the interaction of KLK11 with CCDC25 and other proteins, using STRING version 11.0, revealed that CCDC25 and KLK11 correlated with metastasis-related proteins. In addition, Kaplan-Meier survival curve analysis revealed that a high expression of KLK11 was associated with the poor prognosis of CCA. In conclusion, KLK11 is, as a binding protein for CCDC25, possibly involved in the metastatic process of CCA. KLK11 may be used as a prognostic marker for CCA.https://www.mdpi.com/1420-3049/26/11/3105cholangiocarcinomaCCDC25KLK11immunohistochemistryprognostic marker |
| spellingShingle | Saeranee Siriphak Ravinnipa Chanakankun Tanakorn Proungvitaya Sittiruk Roytrakul Doungdean Tummanatsakun Wunchana Seubwai Molin Wongwattanakul Siriporn Proungvitaya Kallikrein-11, in Association with Coiled-Coil Domain Containing 25, as a Potential Prognostic Marker for Cholangiocarcinoma with Lymph Node Metastasis Molecules cholangiocarcinoma CCDC25 KLK11 immunohistochemistry prognostic marker |
| title | Kallikrein-11, in Association with Coiled-Coil Domain Containing 25, as a Potential Prognostic Marker for Cholangiocarcinoma with Lymph Node Metastasis |
| title_full | Kallikrein-11, in Association with Coiled-Coil Domain Containing 25, as a Potential Prognostic Marker for Cholangiocarcinoma with Lymph Node Metastasis |
| title_fullStr | Kallikrein-11, in Association with Coiled-Coil Domain Containing 25, as a Potential Prognostic Marker for Cholangiocarcinoma with Lymph Node Metastasis |
| title_full_unstemmed | Kallikrein-11, in Association with Coiled-Coil Domain Containing 25, as a Potential Prognostic Marker for Cholangiocarcinoma with Lymph Node Metastasis |
| title_short | Kallikrein-11, in Association with Coiled-Coil Domain Containing 25, as a Potential Prognostic Marker for Cholangiocarcinoma with Lymph Node Metastasis |
| title_sort | kallikrein 11 in association with coiled coil domain containing 25 as a potential prognostic marker for cholangiocarcinoma with lymph node metastasis |
| topic | cholangiocarcinoma CCDC25 KLK11 immunohistochemistry prognostic marker |
| url | https://www.mdpi.com/1420-3049/26/11/3105 |
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