MILIACINE INFLUENCE ON THE BIOFILM FORMATION OF BACTERIA
Aim. The comparative estimation of miliacine influence on the biofilm formation of bacteria. Materials and methods. The objects of investigation were the clinical isolates of Salmonella enteritidis (28), Salmonella typhimurium (24), Klebsiella pneumoniae (8), Pseudomonas aeruginosa (8) and reference...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | Russian |
| Published: |
Central Research Institute for Epidemiology
2016-08-01
|
| Series: | Журнал микробиологии, эпидемиологии и иммунобиологии |
| Subjects: | |
| Online Access: | https://microbiol.elpub.ru/jour/article/view/62 |
| _version_ | 1818081126759006208 |
|---|---|
| author | I. N. Chainikova Yu. V. Filippova B. A. Frolov N. B. Perunova E. V. Ivanova T. A. Bondarenko T. V. Panfilova A. D. Zheleznova Yu. A. Sarycheva O. V. Bukharin |
| author_facet | I. N. Chainikova Yu. V. Filippova B. A. Frolov N. B. Perunova E. V. Ivanova T. A. Bondarenko T. V. Panfilova A. D. Zheleznova Yu. A. Sarycheva O. V. Bukharin |
| author_sort | I. N. Chainikova |
| collection | DOAJ |
| description | Aim. The comparative estimation of miliacine influence on the biofilm formation of bacteria. Materials and methods. The objects of investigation were the clinical isolates of Salmonella enteritidis (28), Salmonella typhimurium (24), Klebsiella pneumoniae (8), Pseudomonas aeruginosa (8) and reference strains of lactobacilli (5) and bifidobacteria (3). Miliacin was obtained from crystals of millet oil. Antibacterial activity of miliacin was detected by the method of serial dilutions. For investigation of biofilms miliacin in 100 and 50 mkg/ml concentrations was used. Miliacin was diluted in Twin-21. Biofilm formation was studied by method of O'Toole G.A., Kolter R. (1998) using spectrophotometer Elx 808 (BioTek, USA). The morphometry of biofilms was conducted by atomic force microscopy with the use of scanning probe microscope SMM-2000. Results. Miliacin and its solvent did not influence the growth of bacteria. Maximum sensivity of biofilms to miliacin was detected in K. pneumoniae and P. aeruginosa, minimal - in S. enteritidis. Miliacin did not influence the biofilm formation in strains of lactobacilli and bifidobacteria. Conclusion. Miliacin in addition to immunotropic activity, detected earlier, can inhibit the biofilms of opportunistic and pathogenic bacteria without influence on the biofilm formation of representatives of usual flora. |
| first_indexed | 2024-12-10T19:01:15Z |
| format | Article |
| id | doaj.art-01c72465ae5541c6824b20fea8aff6b3 |
| institution | Directory Open Access Journal |
| issn | 0372-9311 2686-7613 |
| language | Russian |
| last_indexed | 2024-12-10T19:01:15Z |
| publishDate | 2016-08-01 |
| publisher | Central Research Institute for Epidemiology |
| record_format | Article |
| series | Журнал микробиологии, эпидемиологии и иммунобиологии |
| spelling | doaj.art-01c72465ae5541c6824b20fea8aff6b32022-12-22T01:37:00ZrusCentral Research Institute for EpidemiologyЖурнал микробиологии, эпидемиологии и иммунобиологии0372-93112686-76132016-08-01043910.36233/0372-9311-2016-4-3-962MILIACINE INFLUENCE ON THE BIOFILM FORMATION OF BACTERIAI. N. Chainikova0Yu. V. Filippova1B. A. Frolov2N. B. Perunova3E. V. Ivanova4T. A. Bondarenko5T. V. Panfilova6A. D. Zheleznova7Yu. A. Sarycheva8O. V. Bukharin9Institute of Cellular and Intracellular Symbiosis, Orenburg State Medical UniversityOrenburg State Medical UniversityOrenburg State Medical UniversityInstitute of Cellular and Intracellular SymbiosisInstitute of Cellular and Intracellular SymbiosisInstitute of Cellular and Intracellular SymbiosisOrenburg State Medical UniversityOrenburg State Medical UniversityOrenburg State Medical UniversityInstitute of Cellular and Intracellular SymbiosisAim. The comparative estimation of miliacine influence on the biofilm formation of bacteria. Materials and methods. The objects of investigation were the clinical isolates of Salmonella enteritidis (28), Salmonella typhimurium (24), Klebsiella pneumoniae (8), Pseudomonas aeruginosa (8) and reference strains of lactobacilli (5) and bifidobacteria (3). Miliacin was obtained from crystals of millet oil. Antibacterial activity of miliacin was detected by the method of serial dilutions. For investigation of biofilms miliacin in 100 and 50 mkg/ml concentrations was used. Miliacin was diluted in Twin-21. Biofilm formation was studied by method of O'Toole G.A., Kolter R. (1998) using spectrophotometer Elx 808 (BioTek, USA). The morphometry of biofilms was conducted by atomic force microscopy with the use of scanning probe microscope SMM-2000. Results. Miliacin and its solvent did not influence the growth of bacteria. Maximum sensivity of biofilms to miliacin was detected in K. pneumoniae and P. aeruginosa, minimal - in S. enteritidis. Miliacin did not influence the biofilm formation in strains of lactobacilli and bifidobacteria. Conclusion. Miliacin in addition to immunotropic activity, detected earlier, can inhibit the biofilms of opportunistic and pathogenic bacteria without influence on the biofilm formation of representatives of usual flora.https://microbiol.elpub.ru/jour/article/view/62triterpenoidsmiliacinbiofilm formation of bacteria |
| spellingShingle | I. N. Chainikova Yu. V. Filippova B. A. Frolov N. B. Perunova E. V. Ivanova T. A. Bondarenko T. V. Panfilova A. D. Zheleznova Yu. A. Sarycheva O. V. Bukharin MILIACINE INFLUENCE ON THE BIOFILM FORMATION OF BACTERIA Журнал микробиологии, эпидемиологии и иммунобиологии triterpenoids miliacin biofilm formation of bacteria |
| title | MILIACINE INFLUENCE ON THE BIOFILM FORMATION OF BACTERIA |
| title_full | MILIACINE INFLUENCE ON THE BIOFILM FORMATION OF BACTERIA |
| title_fullStr | MILIACINE INFLUENCE ON THE BIOFILM FORMATION OF BACTERIA |
| title_full_unstemmed | MILIACINE INFLUENCE ON THE BIOFILM FORMATION OF BACTERIA |
| title_short | MILIACINE INFLUENCE ON THE BIOFILM FORMATION OF BACTERIA |
| title_sort | miliacine influence on the biofilm formation of bacteria |
| topic | triterpenoids miliacin biofilm formation of bacteria |
| url | https://microbiol.elpub.ru/jour/article/view/62 |
| work_keys_str_mv | AT inchainikova miliacineinfluenceonthebiofilmformationofbacteria AT yuvfilippova miliacineinfluenceonthebiofilmformationofbacteria AT bafrolov miliacineinfluenceonthebiofilmformationofbacteria AT nbperunova miliacineinfluenceonthebiofilmformationofbacteria AT evivanova miliacineinfluenceonthebiofilmformationofbacteria AT tabondarenko miliacineinfluenceonthebiofilmformationofbacteria AT tvpanfilova miliacineinfluenceonthebiofilmformationofbacteria AT adzheleznova miliacineinfluenceonthebiofilmformationofbacteria AT yuasarycheva miliacineinfluenceonthebiofilmformationofbacteria AT ovbukharin miliacineinfluenceonthebiofilmformationofbacteria |