Identification and Molecular Characterization of Giant Liver Fluke (<i>Fascioloides magna</i>) Infection in European Fallow Deer (<i>Dama dama</i>) in Romania—First Report
Fascioloidosis is a parasitic disease of primary wild and domestic ruminants, caused by giant liver fluke, <i>Fascioloides magna</i>. The definitive host of the liver fluke in its area of origin (North America) is the white-tailed deer (<i>Odocoileus virginianus</i>). In Euro...
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MDPI AG
2024-03-01
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| author | Dan-Cornel Popovici Gheorghe Dărăbuș Ana-Maria Marin Ovidiu Ionescu Maria Monica Florina Moraru Mirela Imre Emil Tîrziu Narcisa Mederle |
| author_facet | Dan-Cornel Popovici Gheorghe Dărăbuș Ana-Maria Marin Ovidiu Ionescu Maria Monica Florina Moraru Mirela Imre Emil Tîrziu Narcisa Mederle |
| author_sort | Dan-Cornel Popovici |
| collection | DOAJ |
| description | Fascioloidosis is a parasitic disease of primary wild and domestic ruminants, caused by giant liver fluke, <i>Fascioloides magna</i>. The definitive host of the liver fluke in its area of origin (North America) is the white-tailed deer (<i>Odocoileus virginianus</i>). In Europe, the red deer (<i>Cervus elaphus</i>) and European fallow deer (<i>Dama dama</i>) are definitive hosts and the most sensitive hosts to <i>F. magna</i> infection, on which the parasite exerts serious pathogenic effects. In this study, we analyzed fecal samples and livers of 72 <i>D. dama</i> from 11 hunting grounds in Arad County, Romania. Of the 72 fecal samples and livers from <i>D. dama</i>, trematodes of the genus <i>Fascioloides</i> were identified in four (5.56%). Sequencing revealed that the trematodes identified in the samples were similar to the sequence of <i>F. magna</i> (GenBank no. EF534992.1, DQ683545.1, KU232369.1). The sequence obtained from the molecular analysis has been deposited in GenBank<sup>®</sup> under accession number OQ689976.1. This study describes the first report of giant liver fluke (<i>F. magna</i>) infection in <i>D. dama</i> in Romania. |
| first_indexed | 2024-04-24T17:58:55Z |
| format | Article |
| id | doaj.art-01d517641740482182a32390e881a697 |
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| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-04-24T17:58:55Z |
| publishDate | 2024-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-01d517641740482182a32390e881a6972024-03-27T13:55:43ZengMDPI AGMicroorganisms2076-26072024-03-0112352710.3390/microorganisms12030527Identification and Molecular Characterization of Giant Liver Fluke (<i>Fascioloides magna</i>) Infection in European Fallow Deer (<i>Dama dama</i>) in Romania—First ReportDan-Cornel Popovici0Gheorghe Dărăbuș1Ana-Maria Marin2Ovidiu Ionescu3Maria Monica Florina Moraru4Mirela Imre5Emil Tîrziu6Narcisa Mederle7Forestry Faculty, Transilvania University Brasov, Sirul Beethoven, 500123 Brasov, RomaniaFaculty of Veterinary Medicine, University of Life Sciences “King Michael I” from Timisoara, 300645 Timisoara, RomaniaFaculty of Veterinary Medicine, University of Life Sciences “King Michael I” from Timisoara, 300645 Timisoara, RomaniaForestry Faculty, Transilvania University Brasov, Sirul Beethoven, 500123 Brasov, RomaniaFaculty of Veterinary Medicine, University of Life Sciences “King Michael I” from Timisoara, 300645 Timisoara, RomaniaFaculty of Veterinary Medicine, University of Life Sciences “King Michael I” from Timisoara, 300645 Timisoara, RomaniaFaculty of Veterinary Medicine, University of Life Sciences “King Michael I” from Timisoara, 300645 Timisoara, RomaniaFaculty of Veterinary Medicine, University of Life Sciences “King Michael I” from Timisoara, 300645 Timisoara, RomaniaFascioloidosis is a parasitic disease of primary wild and domestic ruminants, caused by giant liver fluke, <i>Fascioloides magna</i>. The definitive host of the liver fluke in its area of origin (North America) is the white-tailed deer (<i>Odocoileus virginianus</i>). In Europe, the red deer (<i>Cervus elaphus</i>) and European fallow deer (<i>Dama dama</i>) are definitive hosts and the most sensitive hosts to <i>F. magna</i> infection, on which the parasite exerts serious pathogenic effects. In this study, we analyzed fecal samples and livers of 72 <i>D. dama</i> from 11 hunting grounds in Arad County, Romania. Of the 72 fecal samples and livers from <i>D. dama</i>, trematodes of the genus <i>Fascioloides</i> were identified in four (5.56%). Sequencing revealed that the trematodes identified in the samples were similar to the sequence of <i>F. magna</i> (GenBank no. EF534992.1, DQ683545.1, KU232369.1). The sequence obtained from the molecular analysis has been deposited in GenBank<sup>®</sup> under accession number OQ689976.1. This study describes the first report of giant liver fluke (<i>F. magna</i>) infection in <i>D. dama</i> in Romania.https://www.mdpi.com/2076-2607/12/3/527fascioloidosisparasitic diseaseswild ruminants |
| spellingShingle | Dan-Cornel Popovici Gheorghe Dărăbuș Ana-Maria Marin Ovidiu Ionescu Maria Monica Florina Moraru Mirela Imre Emil Tîrziu Narcisa Mederle Identification and Molecular Characterization of Giant Liver Fluke (<i>Fascioloides magna</i>) Infection in European Fallow Deer (<i>Dama dama</i>) in Romania—First Report Microorganisms fascioloidosis parasitic diseases wild ruminants |
| title | Identification and Molecular Characterization of Giant Liver Fluke (<i>Fascioloides magna</i>) Infection in European Fallow Deer (<i>Dama dama</i>) in Romania—First Report |
| title_full | Identification and Molecular Characterization of Giant Liver Fluke (<i>Fascioloides magna</i>) Infection in European Fallow Deer (<i>Dama dama</i>) in Romania—First Report |
| title_fullStr | Identification and Molecular Characterization of Giant Liver Fluke (<i>Fascioloides magna</i>) Infection in European Fallow Deer (<i>Dama dama</i>) in Romania—First Report |
| title_full_unstemmed | Identification and Molecular Characterization of Giant Liver Fluke (<i>Fascioloides magna</i>) Infection in European Fallow Deer (<i>Dama dama</i>) in Romania—First Report |
| title_short | Identification and Molecular Characterization of Giant Liver Fluke (<i>Fascioloides magna</i>) Infection in European Fallow Deer (<i>Dama dama</i>) in Romania—First Report |
| title_sort | identification and molecular characterization of giant liver fluke i fascioloides magna i infection in european fallow deer i dama dama i in romania first report |
| topic | fascioloidosis parasitic diseases wild ruminants |
| url | https://www.mdpi.com/2076-2607/12/3/527 |
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