Cloning of the wheat leaf rust resistance gene Lr47 introgressed from Aegilops speltoides
Abstract Leaf rust, caused by Puccinia triticina Eriksson (Pt), is one of the most severe foliar diseases of wheat. Breeding for leaf rust resistance is a practical and sustainable method to control this devastating disease. Here, we report the identification of Lr47, a broadly effective leaf rust r...
| Main Authors: | , , , , , , , , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2023-09-01
|
| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-023-41833-2 |
| _version_ | 1797557982943248384 |
|---|---|
| author | Hongna Li Lei Hua Shuqing Zhao Ming Hao Rui Song Shuyong Pang Yanna Liu Hong Chen Wenjun Zhang Tao Shen Jin-Ying Gou Hailiang Mao Guiping Wang Xiaohua Hao Jian Li Baoxing Song Caixia Lan Zaifeng Li Xing Wang Deng Jorge Dubcovsky Xiaodong Wang Shisheng Chen |
| author_facet | Hongna Li Lei Hua Shuqing Zhao Ming Hao Rui Song Shuyong Pang Yanna Liu Hong Chen Wenjun Zhang Tao Shen Jin-Ying Gou Hailiang Mao Guiping Wang Xiaohua Hao Jian Li Baoxing Song Caixia Lan Zaifeng Li Xing Wang Deng Jorge Dubcovsky Xiaodong Wang Shisheng Chen |
| author_sort | Hongna Li |
| collection | DOAJ |
| description | Abstract Leaf rust, caused by Puccinia triticina Eriksson (Pt), is one of the most severe foliar diseases of wheat. Breeding for leaf rust resistance is a practical and sustainable method to control this devastating disease. Here, we report the identification of Lr47, a broadly effective leaf rust resistance gene introgressed into wheat from Aegilops speltoides. Lr47 encodes a coiled-coil nucleotide-binding leucine-rich repeat protein that is both necessary and sufficient to confer Pt resistance, as demonstrated by loss-of-function mutations and transgenic complementation. Lr47 introgression lines with no or reduced linkage drag are generated using the Pairing homoeologous1 mutation, and a diagnostic molecular marker for Lr47 is developed. The coiled-coil domain of the Lr47 protein is unable to induce cell death, nor does it have self-protein interaction. The cloning of Lr47 expands the number of leaf rust resistance genes that can be incorporated into multigene transgenic cassettes to control this devastating disease. |
| first_indexed | 2024-03-10T17:23:59Z |
| format | Article |
| id | doaj.art-01d5fa72c37345f0851253825df18807 |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-03-10T17:23:59Z |
| publishDate | 2023-09-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-01d5fa72c37345f0851253825df188072023-11-20T10:13:44ZengNature PortfolioNature Communications2041-17232023-09-0114111510.1038/s41467-023-41833-2Cloning of the wheat leaf rust resistance gene Lr47 introgressed from Aegilops speltoidesHongna Li0Lei Hua1Shuqing Zhao2Ming Hao3Rui Song4Shuyong Pang5Yanna Liu6Hong Chen7Wenjun Zhang8Tao Shen9Jin-Ying Gou10Hailiang Mao11Guiping Wang12Xiaohua Hao13Jian Li14Baoxing Song15Caixia Lan16Zaifeng Li17Xing Wang Deng18Jorge Dubcovsky19Xiaodong Wang20Shisheng Chen21National Key Laboratory of Wheat Improvement, Peking University Institute of Advanced Agricultural Sciences, Shandong Laboratory of Advanced Agricultural Sciences in WeifangNational Key Laboratory of Wheat Improvement, Peking University Institute of Advanced Agricultural Sciences, Shandong Laboratory of Advanced Agricultural Sciences in WeifangState Key Laboratory of North China Crop Improvement and Regulation, College of Plant Protection, Hebei Agricultural UniversityTriticeae Research Institute, Sichuan Agricultural UniversityNational Key Laboratory of Wheat Improvement, Peking University Institute of Advanced Agricultural Sciences, Shandong Laboratory of Advanced Agricultural Sciences in WeifangNational Key Laboratory of Wheat Improvement, Peking University Institute of Advanced Agricultural Sciences, Shandong Laboratory of Advanced Agricultural Sciences in WeifangNational Key Laboratory of Wheat Improvement, Peking University Institute of Advanced Agricultural Sciences, Shandong Laboratory of Advanced Agricultural Sciences in WeifangTriticeae Research Institute, Sichuan Agricultural UniversityDepartment of Plant Sciences, University of CaliforniaNational Key Laboratory of Wheat Improvement, Peking University Institute of Advanced Agricultural Sciences, Shandong Laboratory of Advanced Agricultural Sciences in WeifangKey Laboratory of Crop Heterosis and Utilization (MOE) and Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural UniversityNational Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural UniversityNational Key Laboratory of Wheat Improvement, Peking University Institute of Advanced Agricultural Sciences, Shandong Laboratory of Advanced Agricultural Sciences in WeifangNational Key Laboratory of Wheat Improvement, Peking University Institute of Advanced Agricultural Sciences, Shandong Laboratory of Advanced Agricultural Sciences in WeifangNational Key Laboratory of Wheat Improvement, Peking University Institute of Advanced Agricultural Sciences, Shandong Laboratory of Advanced Agricultural Sciences in WeifangNational Key Laboratory of Wheat Improvement, Peking University Institute of Advanced Agricultural Sciences, Shandong Laboratory of Advanced Agricultural Sciences in WeifangNational Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural UniversityState Key Laboratory of North China Crop Improvement and Regulation, College of Plant Protection, Hebei Agricultural UniversityNational Key Laboratory of Wheat Improvement, Peking University Institute of Advanced Agricultural Sciences, Shandong Laboratory of Advanced Agricultural Sciences in WeifangDepartment of Plant Sciences, University of CaliforniaState Key Laboratory of North China Crop Improvement and Regulation, College of Plant Protection, Hebei Agricultural UniversityNational Key Laboratory of Wheat Improvement, Peking University Institute of Advanced Agricultural Sciences, Shandong Laboratory of Advanced Agricultural Sciences in WeifangAbstract Leaf rust, caused by Puccinia triticina Eriksson (Pt), is one of the most severe foliar diseases of wheat. Breeding for leaf rust resistance is a practical and sustainable method to control this devastating disease. Here, we report the identification of Lr47, a broadly effective leaf rust resistance gene introgressed into wheat from Aegilops speltoides. Lr47 encodes a coiled-coil nucleotide-binding leucine-rich repeat protein that is both necessary and sufficient to confer Pt resistance, as demonstrated by loss-of-function mutations and transgenic complementation. Lr47 introgression lines with no or reduced linkage drag are generated using the Pairing homoeologous1 mutation, and a diagnostic molecular marker for Lr47 is developed. The coiled-coil domain of the Lr47 protein is unable to induce cell death, nor does it have self-protein interaction. The cloning of Lr47 expands the number of leaf rust resistance genes that can be incorporated into multigene transgenic cassettes to control this devastating disease.https://doi.org/10.1038/s41467-023-41833-2 |
| spellingShingle | Hongna Li Lei Hua Shuqing Zhao Ming Hao Rui Song Shuyong Pang Yanna Liu Hong Chen Wenjun Zhang Tao Shen Jin-Ying Gou Hailiang Mao Guiping Wang Xiaohua Hao Jian Li Baoxing Song Caixia Lan Zaifeng Li Xing Wang Deng Jorge Dubcovsky Xiaodong Wang Shisheng Chen Cloning of the wheat leaf rust resistance gene Lr47 introgressed from Aegilops speltoides Nature Communications |
| title | Cloning of the wheat leaf rust resistance gene Lr47 introgressed from Aegilops speltoides |
| title_full | Cloning of the wheat leaf rust resistance gene Lr47 introgressed from Aegilops speltoides |
| title_fullStr | Cloning of the wheat leaf rust resistance gene Lr47 introgressed from Aegilops speltoides |
| title_full_unstemmed | Cloning of the wheat leaf rust resistance gene Lr47 introgressed from Aegilops speltoides |
| title_short | Cloning of the wheat leaf rust resistance gene Lr47 introgressed from Aegilops speltoides |
| title_sort | cloning of the wheat leaf rust resistance gene lr47 introgressed from aegilops speltoides |
| url | https://doi.org/10.1038/s41467-023-41833-2 |
| work_keys_str_mv | AT hongnali cloningofthewheatleafrustresistancegenelr47introgressedfromaegilopsspeltoides AT leihua cloningofthewheatleafrustresistancegenelr47introgressedfromaegilopsspeltoides AT shuqingzhao cloningofthewheatleafrustresistancegenelr47introgressedfromaegilopsspeltoides AT minghao cloningofthewheatleafrustresistancegenelr47introgressedfromaegilopsspeltoides AT ruisong cloningofthewheatleafrustresistancegenelr47introgressedfromaegilopsspeltoides AT shuyongpang cloningofthewheatleafrustresistancegenelr47introgressedfromaegilopsspeltoides AT yannaliu cloningofthewheatleafrustresistancegenelr47introgressedfromaegilopsspeltoides AT hongchen cloningofthewheatleafrustresistancegenelr47introgressedfromaegilopsspeltoides AT wenjunzhang cloningofthewheatleafrustresistancegenelr47introgressedfromaegilopsspeltoides AT taoshen cloningofthewheatleafrustresistancegenelr47introgressedfromaegilopsspeltoides AT jinyinggou cloningofthewheatleafrustresistancegenelr47introgressedfromaegilopsspeltoides AT hailiangmao cloningofthewheatleafrustresistancegenelr47introgressedfromaegilopsspeltoides AT guipingwang cloningofthewheatleafrustresistancegenelr47introgressedfromaegilopsspeltoides AT xiaohuahao cloningofthewheatleafrustresistancegenelr47introgressedfromaegilopsspeltoides AT jianli cloningofthewheatleafrustresistancegenelr47introgressedfromaegilopsspeltoides AT baoxingsong cloningofthewheatleafrustresistancegenelr47introgressedfromaegilopsspeltoides AT caixialan cloningofthewheatleafrustresistancegenelr47introgressedfromaegilopsspeltoides AT zaifengli cloningofthewheatleafrustresistancegenelr47introgressedfromaegilopsspeltoides AT xingwangdeng cloningofthewheatleafrustresistancegenelr47introgressedfromaegilopsspeltoides AT jorgedubcovsky cloningofthewheatleafrustresistancegenelr47introgressedfromaegilopsspeltoides AT xiaodongwang cloningofthewheatleafrustresistancegenelr47introgressedfromaegilopsspeltoides AT shishengchen cloningofthewheatleafrustresistancegenelr47introgressedfromaegilopsspeltoides |