Finding of coagulase negative staphylococci in the herd with an increased number of somatic cells in milk and their antimicrobial susceptibility
Coagulase-negative staphylococci (CNS) are generally considered to be opportunistic pathogens. Controlling CNS mastitis is difficult because the epidemiology is not clear, and the CNS group consists of about 40 different Staphylococcus species. Therefore, the aim of this study was to determ...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Faculty of Veterinary Medicine, Belgrade
2013-01-01
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| Series: | Veterinarski Glasnik |
| Subjects: | |
| Online Access: | http://www.doiserbia.nb.rs/img/doi/0350-2457/2013/0350-24571304175K.pdf |
| _version_ | 1818321684104478720 |
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| author | Katić Vera Rajić-Savić Nataša |
| author_facet | Katić Vera Rajić-Savić Nataša |
| author_sort | Katić Vera |
| collection | DOAJ |
| description | Coagulase-negative staphylococci (CNS) are generally considered to be
opportunistic pathogens. Controlling CNS mastitis is difficult because the
epidemiology is not clear, and the CNS group consists of about 40 different
Staphylococcus species. Therefore, the aim of this study was to determine the
prevalence of coagulasenegative staphylococci in milk of the cows with
subclinical mastitis, as well as to determine different CNS species isolated
from quarter milk samples for their susceptibility to antimicrobials used
commonly for mastitis therapy. On the farm where there was found an increase
of somatic cells in bulk milk, 112 dairy cows were examined by mastitis test.
From 52 udder quarters where mastitis test showed an increase of somatic
cells, milk samples were taken for bacteriological examination. For isolating
the causes of mastitis there was used blood agar. Identification of the
causative agents of mastitis was carried out on the basis of colony
appearance on blood agar and their physiological characteristics.
Coagulasepositive staphylococci sensitivity which cause mastitis was tested
by Kirby Bauer method. For susceptibility testing there were used
commercially produced discs containing: 10 IU penicillin,
amoxicillin/clavulanic acid (20 +10 μg), cloxacillin 25 μg, 30 μg
amoxicillin, cephalexin 30 μg, ceftiofur 30 μg, 15 μg lincomycin, gentamicin
and tetracycline 30 μg. The sensitivity of microorganisms was evaluated on
the basis of inhibition zone diameter recommended by the manufacturer and was
labeled as sensitive (S) moderately sensitive (I) or resistant (R).
Coagulase-negative staphylococci were isolated from 61.53% of samples from
cows with subclinical mastitis, making them the most common cause of
subclinical mastitis. The highest resistance of coagulase-negative
staphylococci was found to penicillin G (58.33% of isolates). Full
sensitivity of coagulase-negative staphylococci was found to amoxicillin /
clavulanic acid (100% of isolates), a good sensitivity to ceftiofur (83.33%
of isolates), cefalexin (70.83% of isolates) and ceftriaxone (41.66% of
isolates). [Projekat Ministarstva nauke Republike Srbije, br. TR 31086] |
| first_indexed | 2024-12-13T10:44:49Z |
| format | Article |
| id | doaj.art-01d869ec4fd24c8b86a6c76e54cd220c |
| institution | Directory Open Access Journal |
| issn | 0350-2457 2406-0771 |
| language | English |
| last_indexed | 2024-12-13T10:44:49Z |
| publishDate | 2013-01-01 |
| publisher | Faculty of Veterinary Medicine, Belgrade |
| record_format | Article |
| series | Veterinarski Glasnik |
| spelling | doaj.art-01d869ec4fd24c8b86a6c76e54cd220c2022-12-21T23:50:15ZengFaculty of Veterinary Medicine, BelgradeVeterinarski Glasnik0350-24572406-07712013-01-01673-417518510.2298/VETGL1304175K0350-24571304175KFinding of coagulase negative staphylococci in the herd with an increased number of somatic cells in milk and their antimicrobial susceptibilityKatić Vera0Rajić-Savić Nataša1Fakultet Veterinarske medicine, BeogradPKB Korporacija, Dijagnostička laboratorija, Padinska Skela, BeogradCoagulase-negative staphylococci (CNS) are generally considered to be opportunistic pathogens. Controlling CNS mastitis is difficult because the epidemiology is not clear, and the CNS group consists of about 40 different Staphylococcus species. Therefore, the aim of this study was to determine the prevalence of coagulasenegative staphylococci in milk of the cows with subclinical mastitis, as well as to determine different CNS species isolated from quarter milk samples for their susceptibility to antimicrobials used commonly for mastitis therapy. On the farm where there was found an increase of somatic cells in bulk milk, 112 dairy cows were examined by mastitis test. From 52 udder quarters where mastitis test showed an increase of somatic cells, milk samples were taken for bacteriological examination. For isolating the causes of mastitis there was used blood agar. Identification of the causative agents of mastitis was carried out on the basis of colony appearance on blood agar and their physiological characteristics. Coagulasepositive staphylococci sensitivity which cause mastitis was tested by Kirby Bauer method. For susceptibility testing there were used commercially produced discs containing: 10 IU penicillin, amoxicillin/clavulanic acid (20 +10 μg), cloxacillin 25 μg, 30 μg amoxicillin, cephalexin 30 μg, ceftiofur 30 μg, 15 μg lincomycin, gentamicin and tetracycline 30 μg. The sensitivity of microorganisms was evaluated on the basis of inhibition zone diameter recommended by the manufacturer and was labeled as sensitive (S) moderately sensitive (I) or resistant (R). Coagulase-negative staphylococci were isolated from 61.53% of samples from cows with subclinical mastitis, making them the most common cause of subclinical mastitis. The highest resistance of coagulase-negative staphylococci was found to penicillin G (58.33% of isolates). Full sensitivity of coagulase-negative staphylococci was found to amoxicillin / clavulanic acid (100% of isolates), a good sensitivity to ceftiofur (83.33% of isolates), cefalexin (70.83% of isolates) and ceftriaxone (41.66% of isolates). [Projekat Ministarstva nauke Republike Srbije, br. TR 31086]http://www.doiserbia.nb.rs/img/doi/0350-2457/2013/0350-24571304175K.pdfcoagulase negative staphylococcimastitisantimicrobialsmilksomatic cells |
| spellingShingle | Katić Vera Rajić-Savić Nataša Finding of coagulase negative staphylococci in the herd with an increased number of somatic cells in milk and their antimicrobial susceptibility Veterinarski Glasnik coagulase negative staphylococci mastitis antimicrobials milk somatic cells |
| title | Finding of coagulase negative staphylococci in the herd with an increased number of somatic cells in milk and their antimicrobial susceptibility |
| title_full | Finding of coagulase negative staphylococci in the herd with an increased number of somatic cells in milk and their antimicrobial susceptibility |
| title_fullStr | Finding of coagulase negative staphylococci in the herd with an increased number of somatic cells in milk and their antimicrobial susceptibility |
| title_full_unstemmed | Finding of coagulase negative staphylococci in the herd with an increased number of somatic cells in milk and their antimicrobial susceptibility |
| title_short | Finding of coagulase negative staphylococci in the herd with an increased number of somatic cells in milk and their antimicrobial susceptibility |
| title_sort | finding of coagulase negative staphylococci in the herd with an increased number of somatic cells in milk and their antimicrobial susceptibility |
| topic | coagulase negative staphylococci mastitis antimicrobials milk somatic cells |
| url | http://www.doiserbia.nb.rs/img/doi/0350-2457/2013/0350-24571304175K.pdf |
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