Characterization of a Putative New Member of the Genus <i>Potyvirus</i> from Kudzu (<i>Pueraria montana</i> var. <i>lobata</i>) in Mississippi
Kudzu (<i>Pueraria montana</i> var. <i>lobata</i>), a plant native to Southeastern Asia, has become a major noxious weed covering millions of hectares in the Southern United States. A kudzu patch displaying virus-like symptoms located in Ackerman, northeastern Mississippi (MS...
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| Format: | Article |
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MDPI AG
2023-10-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/15/11/2145 |
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| author | Nina Aboughanem-Sabanadzovic Ronald Christian Stephenson Thomas W. Allen Alan Henn William F. Moore Amanda Lawrence Sead Sabanadzovic |
| author_facet | Nina Aboughanem-Sabanadzovic Ronald Christian Stephenson Thomas W. Allen Alan Henn William F. Moore Amanda Lawrence Sead Sabanadzovic |
| author_sort | Nina Aboughanem-Sabanadzovic |
| collection | DOAJ |
| description | Kudzu (<i>Pueraria montana</i> var. <i>lobata</i>), a plant native to Southeastern Asia, has become a major noxious weed covering millions of hectares in the Southern United States. A kudzu patch displaying virus-like symptoms located in Ackerman, northeastern Mississippi (MS), was used as a source for virus isolation and characterization involving mechanical and vector transmission, ultrastructural observation, surveys, Sanger and high-throughput genome sequencing, and sequence analyses. The results revealed the presence of a new potyvirus in infected kudzu, closely related to wisteria vein mosaic virus (WVMV) and provisionally named kudzu chlorotic ring blotch virus (KudCRBV). Genome features and pairwise comparison with six WVMV genomes currently available in GenBank and three additional isolates from MS sequenced in this work suggest that KudCRBV is likely a member of a new species in the genus <i>Potyvirus</i>. Furthermore, under experimental conditions, KudCRBV was successfully transmitted by cotton and potato aphids and mechanically to soybean and beans. A state-wide survey revealed several kudzu patches infected by the virus in northern MS. |
| first_indexed | 2024-03-09T16:23:14Z |
| format | Article |
| id | doaj.art-01dad4f2859c45859494b7f9eef6c9a2 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-09T16:23:14Z |
| publishDate | 2023-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-01dad4f2859c45859494b7f9eef6c9a22023-11-24T15:10:33ZengMDPI AGViruses1999-49152023-10-011511214510.3390/v15112145Characterization of a Putative New Member of the Genus <i>Potyvirus</i> from Kudzu (<i>Pueraria montana</i> var. <i>lobata</i>) in MississippiNina Aboughanem-Sabanadzovic0Ronald Christian Stephenson1Thomas W. Allen2Alan Henn3William F. Moore4Amanda Lawrence5Sead Sabanadzovic6Institute for Genomics, Biocomputing and Biotechnology, Mississippi State University, Mississippi State, MS 39762, USADepartment of Biochemistry, Molecular Biology, Entomology and Plant Pathology, Mississippi State University, Mississippi State, MS 39762, USADelta Research and Extension Center, Mississippi State University, Stoneville, MS 38776, USADepartment of Biochemistry, Molecular Biology, Entomology and Plant Pathology, Mississippi State University, Mississippi State, MS 39762, USADepartment of Biochemistry, Molecular Biology, Entomology and Plant Pathology, Mississippi State University, Mississippi State, MS 39762, USAInstitute for Imaging and Analytical Technologies, Mississippi State University, Mississippi State, MS 39762, USADepartment of Biochemistry, Molecular Biology, Entomology and Plant Pathology, Mississippi State University, Mississippi State, MS 39762, USAKudzu (<i>Pueraria montana</i> var. <i>lobata</i>), a plant native to Southeastern Asia, has become a major noxious weed covering millions of hectares in the Southern United States. A kudzu patch displaying virus-like symptoms located in Ackerman, northeastern Mississippi (MS), was used as a source for virus isolation and characterization involving mechanical and vector transmission, ultrastructural observation, surveys, Sanger and high-throughput genome sequencing, and sequence analyses. The results revealed the presence of a new potyvirus in infected kudzu, closely related to wisteria vein mosaic virus (WVMV) and provisionally named kudzu chlorotic ring blotch virus (KudCRBV). Genome features and pairwise comparison with six WVMV genomes currently available in GenBank and three additional isolates from MS sequenced in this work suggest that KudCRBV is likely a member of a new species in the genus <i>Potyvirus</i>. Furthermore, under experimental conditions, KudCRBV was successfully transmitted by cotton and potato aphids and mechanically to soybean and beans. A state-wide survey revealed several kudzu patches infected by the virus in northern MS.https://www.mdpi.com/1999-4915/15/11/2145kudzusymptomviruspotyvirushigh-throughput sequencingaphid transmission |
| spellingShingle | Nina Aboughanem-Sabanadzovic Ronald Christian Stephenson Thomas W. Allen Alan Henn William F. Moore Amanda Lawrence Sead Sabanadzovic Characterization of a Putative New Member of the Genus <i>Potyvirus</i> from Kudzu (<i>Pueraria montana</i> var. <i>lobata</i>) in Mississippi Viruses kudzu symptom virus potyvirus high-throughput sequencing aphid transmission |
| title | Characterization of a Putative New Member of the Genus <i>Potyvirus</i> from Kudzu (<i>Pueraria montana</i> var. <i>lobata</i>) in Mississippi |
| title_full | Characterization of a Putative New Member of the Genus <i>Potyvirus</i> from Kudzu (<i>Pueraria montana</i> var. <i>lobata</i>) in Mississippi |
| title_fullStr | Characterization of a Putative New Member of the Genus <i>Potyvirus</i> from Kudzu (<i>Pueraria montana</i> var. <i>lobata</i>) in Mississippi |
| title_full_unstemmed | Characterization of a Putative New Member of the Genus <i>Potyvirus</i> from Kudzu (<i>Pueraria montana</i> var. <i>lobata</i>) in Mississippi |
| title_short | Characterization of a Putative New Member of the Genus <i>Potyvirus</i> from Kudzu (<i>Pueraria montana</i> var. <i>lobata</i>) in Mississippi |
| title_sort | characterization of a putative new member of the genus i potyvirus i from kudzu i pueraria montana i var i lobata i in mississippi |
| topic | kudzu symptom virus potyvirus high-throughput sequencing aphid transmission |
| url | https://www.mdpi.com/1999-4915/15/11/2145 |
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