Feeding Eggs from Hens Immunized with Specific KLH-Conjugated HIV Peptide Candidate Vaccines to Chicks Induces Specific Anti-HIV gp120 and gp41 Antibodies that Neutralize the Original HIV Antigens
Introduction: Isolation of antibodies from the egg yolk of chickens is of particular interest as a source of specific antibodies for oral administration to prevent infections and use them as immunodiagnostic reagents. This study assessed the hypothesis that immunization with human immunodeficiency v...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Pasteur Institute of Iran
2020-12-01
|
| Series: | Vaccine Research |
| Subjects: | |
| Online Access: | http://vacres.pasteur.ac.ir/article-1-262-en.pdf |
| _version_ | 1828349058173894656 |
|---|---|
| author | Justiz Vaillant Ferrer Cosme Fisher Smikle Oliver Pérez |
| author_facet | Justiz Vaillant Ferrer Cosme Fisher Smikle Oliver Pérez |
| author_sort | Justiz Vaillant |
| collection | DOAJ |
| description | Introduction: Isolation of antibodies from the egg yolk of chickens is of particular interest as a source of specific antibodies for oral administration to prevent infections and use them as immunodiagnostic reagents. This study assessed the hypothesis that immunization with human immunodeficiency virus 1(HIV-1) viral peptides could induce a potent immune response that could be evaluated in chicken eggs. Methods: Nine healthy brown Leghorn layer hens (3 per HIV immunogen), aged 7 months, were immunized intramuscularly at multiple sites on the breast with specific KLH-conjugated HIV peptide candidate vaccines. The anti-HIV antibody response was measured using ELISA. Chicks were fed HIV hyper-immune eggs and their blood was collected for testing for anti-anti-idiotypic antibodies that neutralize the original HIV antigen using an inhibition ELISA. Results: The immunogenicity results showed that HIV peptide vaccines effectively produced strong anti-HIV immune responses in immunized brown Leghorn layer hens. The binding inhibition assay showed that 13.9%–20.3% inhibition of the binding of avian anti-HIV gp 41 (fragment 579-601) or anti-HIV-gp120 antibodies (Ab-1) to immobilized HIVgp41, HIVgp120 peptide by anti-HIV gp 41 (fragment 579-601) or anti-HIV gp120 (fragments 308-331 or 421-438) antibodies present in serum sample replicates of chicks tested, suggesting that the chicks anti-HIV gp 41 (fragment 579-601) or anti-HIV-gp120 antibodies (fragments 308-331 or 421-438) were anti-anti-idiotypic antibodies. This inhibition was not observed in the sera of chicks that were not fed with the hyperimmune eggs. Conclusion: Feeding chicks with hyperimmune eggs could potentially stimulate the production of anti-anti-idiotypic antibodies that can neutralize the original HIV antigen (gp120 or gp41). This could be an avenue for immunotherapy to improve the fight against HIV infections. However, more studies and clinical trials are required to demonstrate similar human immune responses. |
| first_indexed | 2024-04-14T01:03:54Z |
| format | Article |
| id | doaj.art-01dcbc09456b42c79d9cba09d733df4f |
| institution | Directory Open Access Journal |
| issn | 2383-2819 2423-4923 |
| language | English |
| last_indexed | 2024-04-14T01:03:54Z |
| publishDate | 2020-12-01 |
| publisher | Pasteur Institute of Iran |
| record_format | Article |
| series | Vaccine Research |
| spelling | doaj.art-01dcbc09456b42c79d9cba09d733df4f2022-12-22T02:21:18ZengPasteur Institute of IranVaccine Research2383-28192423-49232020-12-0172929610.52547/vacres.7.2.92Feeding Eggs from Hens Immunized with Specific KLH-Conjugated HIV Peptide Candidate Vaccines to Chicks Induces Specific Anti-HIV gp120 and gp41 Antibodies that Neutralize the Original HIV AntigensJustiz Vaillant0https://orcid.org/0000-0002-3486-1963Ferrer Cosme1Fisher Smikle2https://orcid.org/0000-0003-1259-2384Oliver Pérez3https://orcid.org/0000-0002-8117-4939Department of Para-Clinical Sciences, Faculty of Medical Sciences, The University of the West Indies. St. Augustine, Trinidad and Tobago,West Indies.Higher Institute of Medical Sciences of Santiago de Cuba, Santiago de Cuba, CubaDepartment of Microbiology, University Hospital of the West Indies, Mona Campus, Kingston, JamaicaDepartment of Immunology, Instituto de Ciencias Básicas y Preclinicas “Victoria de Girón”, Havana, CubaIntroduction: Isolation of antibodies from the egg yolk of chickens is of particular interest as a source of specific antibodies for oral administration to prevent infections and use them as immunodiagnostic reagents. This study assessed the hypothesis that immunization with human immunodeficiency virus 1(HIV-1) viral peptides could induce a potent immune response that could be evaluated in chicken eggs. Methods: Nine healthy brown Leghorn layer hens (3 per HIV immunogen), aged 7 months, were immunized intramuscularly at multiple sites on the breast with specific KLH-conjugated HIV peptide candidate vaccines. The anti-HIV antibody response was measured using ELISA. Chicks were fed HIV hyper-immune eggs and their blood was collected for testing for anti-anti-idiotypic antibodies that neutralize the original HIV antigen using an inhibition ELISA. Results: The immunogenicity results showed that HIV peptide vaccines effectively produced strong anti-HIV immune responses in immunized brown Leghorn layer hens. The binding inhibition assay showed that 13.9%–20.3% inhibition of the binding of avian anti-HIV gp 41 (fragment 579-601) or anti-HIV-gp120 antibodies (Ab-1) to immobilized HIVgp41, HIVgp120 peptide by anti-HIV gp 41 (fragment 579-601) or anti-HIV gp120 (fragments 308-331 or 421-438) antibodies present in serum sample replicates of chicks tested, suggesting that the chicks anti-HIV gp 41 (fragment 579-601) or anti-HIV-gp120 antibodies (fragments 308-331 or 421-438) were anti-anti-idiotypic antibodies. This inhibition was not observed in the sera of chicks that were not fed with the hyperimmune eggs. Conclusion: Feeding chicks with hyperimmune eggs could potentially stimulate the production of anti-anti-idiotypic antibodies that can neutralize the original HIV antigen (gp120 or gp41). This could be an avenue for immunotherapy to improve the fight against HIV infections. However, more studies and clinical trials are required to demonstrate similar human immune responses.http://vacres.pasteur.ac.ir/article-1-262-en.pdfvaccinologyanti-idiotypic antibodieselisahuman immunodeficiency virusviral immunity |
| spellingShingle | Justiz Vaillant Ferrer Cosme Fisher Smikle Oliver Pérez Feeding Eggs from Hens Immunized with Specific KLH-Conjugated HIV Peptide Candidate Vaccines to Chicks Induces Specific Anti-HIV gp120 and gp41 Antibodies that Neutralize the Original HIV Antigens Vaccine Research vaccinology anti-idiotypic antibodies elisa human immunodeficiency virus viral immunity |
| title | Feeding Eggs from Hens Immunized with Specific KLH-Conjugated HIV Peptide Candidate Vaccines to Chicks Induces Specific Anti-HIV gp120 and gp41 Antibodies that Neutralize the Original HIV Antigens |
| title_full | Feeding Eggs from Hens Immunized with Specific KLH-Conjugated HIV Peptide Candidate Vaccines to Chicks Induces Specific Anti-HIV gp120 and gp41 Antibodies that Neutralize the Original HIV Antigens |
| title_fullStr | Feeding Eggs from Hens Immunized with Specific KLH-Conjugated HIV Peptide Candidate Vaccines to Chicks Induces Specific Anti-HIV gp120 and gp41 Antibodies that Neutralize the Original HIV Antigens |
| title_full_unstemmed | Feeding Eggs from Hens Immunized with Specific KLH-Conjugated HIV Peptide Candidate Vaccines to Chicks Induces Specific Anti-HIV gp120 and gp41 Antibodies that Neutralize the Original HIV Antigens |
| title_short | Feeding Eggs from Hens Immunized with Specific KLH-Conjugated HIV Peptide Candidate Vaccines to Chicks Induces Specific Anti-HIV gp120 and gp41 Antibodies that Neutralize the Original HIV Antigens |
| title_sort | feeding eggs from hens immunized with specific klh conjugated hiv peptide candidate vaccines to chicks induces specific anti hiv gp120 and gp41 antibodies that neutralize the original hiv antigens |
| topic | vaccinology anti-idiotypic antibodies elisa human immunodeficiency virus viral immunity |
| url | http://vacres.pasteur.ac.ir/article-1-262-en.pdf |
| work_keys_str_mv | AT justizvaillant feedingeggsfromhensimmunizedwithspecificklhconjugatedhivpeptidecandidatevaccinestochicksinducesspecificantihivgp120andgp41antibodiesthatneutralizetheoriginalhivantigens AT ferrercosme feedingeggsfromhensimmunizedwithspecificklhconjugatedhivpeptidecandidatevaccinestochicksinducesspecificantihivgp120andgp41antibodiesthatneutralizetheoriginalhivantigens AT fishersmikle feedingeggsfromhensimmunizedwithspecificklhconjugatedhivpeptidecandidatevaccinestochicksinducesspecificantihivgp120andgp41antibodiesthatneutralizetheoriginalhivantigens AT oliverperez feedingeggsfromhensimmunizedwithspecificklhconjugatedhivpeptidecandidatevaccinestochicksinducesspecificantihivgp120andgp41antibodiesthatneutralizetheoriginalhivantigens |