Molecular Typing of <i>Neisseria gonorrhoeae</i> Clinical Isolates in Russia, 2018–2019: A Link Between <i>penA</i> Alleles and NG-MAST Types
This work aimed to study <i>penA</i> gene polymorphisms in clinical isolates of <i>Neisseria gonorrhoeae</i> collected in Russia in 2018–2019 and the contribution of the <i>penA</i> allele type to susceptibility to β-lactam antibiotics. A total of 182 isolates wer...
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MDPI AG
2020-11-01
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| Series: | Pathogens |
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| Online Access: | https://www.mdpi.com/2076-0817/9/11/941 |
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| author | Ilya Kandinov Ekaterina Dementieva Dmitry Kravtsov Alexander Chestkov Alexey Kubanov Victoria Solomka Dmitry Deryabin Dmitry Gryadunov Boris Shaskolskiy |
| author_facet | Ilya Kandinov Ekaterina Dementieva Dmitry Kravtsov Alexander Chestkov Alexey Kubanov Victoria Solomka Dmitry Deryabin Dmitry Gryadunov Boris Shaskolskiy |
| author_sort | Ilya Kandinov |
| collection | DOAJ |
| description | This work aimed to study <i>penA</i> gene polymorphisms in clinical isolates of <i>Neisseria gonorrhoeae</i> collected in Russia in 2018–2019 and the contribution of the <i>penA</i> allele type to susceptibility to β-lactam antibiotics. A total of 182 isolates were analyzed. <i>penA</i> allele types were determined by sequencing, and the minimum inhibitory concentrations (MICs) of benzylpenicillin and ceftriaxone were measured. The influence of genetic factors on MICs was evaluated by regression analysis. All isolates were susceptible to ceftriaxone, and 40.1% of isolates were susceptible to penicillin. Eleven <i>penA</i> allele types were identified. The mosaic type XXXIV <i>penA</i> allele and the Gly120Lys substitution in PorB made the greatest contributions to increasing the ceftriaxone MIC; the presence of the <i>bla</i><sub>TEM</sub> plasmid, Gly120Asp, Ala121Gly/Asn substitutions in PorB, and the adenine deletion in the promoter region of the <i>mtrR</i> gene caused an increase in the penicillin MIC. Among 61 NG-MAST types identified, the most frequent were types 228, 807, 9486, 1993, and 6226. A link between <i>penA</i> alleles and <i>Neisseria gonorrhoeae</i> multi-antigen sequence typing (NG-MAST) types was established. Resistance to two groups of β-lactam antibiotics was associated with non-identical changes in <i>penA</i> alleles. To prevent the emergence of ceftriaxone resistance in Russia, NG-MAST genotyping must be supplemented with <i>penA</i> allele analysis. |
| first_indexed | 2024-03-10T14:54:27Z |
| format | Article |
| id | doaj.art-01ddc5ba1975494cbed6c2b3363cbffc |
| institution | Directory Open Access Journal |
| issn | 2076-0817 |
| language | English |
| last_indexed | 2024-03-10T14:54:27Z |
| publishDate | 2020-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pathogens |
| spelling | doaj.art-01ddc5ba1975494cbed6c2b3363cbffc2023-11-20T20:40:10ZengMDPI AGPathogens2076-08172020-11-0191194110.3390/pathogens9110941Molecular Typing of <i>Neisseria gonorrhoeae</i> Clinical Isolates in Russia, 2018–2019: A Link Between <i>penA</i> Alleles and NG-MAST TypesIlya Kandinov0Ekaterina Dementieva1Dmitry Kravtsov2Alexander Chestkov3Alexey Kubanov4Victoria Solomka5Dmitry Deryabin6Dmitry Gryadunov7Boris Shaskolskiy8Center for Precision Genome Editing and Genetic Technologies for Biomedicine, Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 119991 Moscow, RussiaCenter for Precision Genome Editing and Genetic Technologies for Biomedicine, Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 119991 Moscow, RussiaCenter for Precision Genome Editing and Genetic Technologies for Biomedicine, Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 119991 Moscow, RussiaState Research Center of Dermatovenerology and Cosmetology, Russian Ministry of Health, 107076 Moscow, RussiaState Research Center of Dermatovenerology and Cosmetology, Russian Ministry of Health, 107076 Moscow, RussiaState Research Center of Dermatovenerology and Cosmetology, Russian Ministry of Health, 107076 Moscow, RussiaState Research Center of Dermatovenerology and Cosmetology, Russian Ministry of Health, 107076 Moscow, RussiaCenter for Precision Genome Editing and Genetic Technologies for Biomedicine, Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 119991 Moscow, RussiaCenter for Precision Genome Editing and Genetic Technologies for Biomedicine, Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 119991 Moscow, RussiaThis work aimed to study <i>penA</i> gene polymorphisms in clinical isolates of <i>Neisseria gonorrhoeae</i> collected in Russia in 2018–2019 and the contribution of the <i>penA</i> allele type to susceptibility to β-lactam antibiotics. A total of 182 isolates were analyzed. <i>penA</i> allele types were determined by sequencing, and the minimum inhibitory concentrations (MICs) of benzylpenicillin and ceftriaxone were measured. The influence of genetic factors on MICs was evaluated by regression analysis. All isolates were susceptible to ceftriaxone, and 40.1% of isolates were susceptible to penicillin. Eleven <i>penA</i> allele types were identified. The mosaic type XXXIV <i>penA</i> allele and the Gly120Lys substitution in PorB made the greatest contributions to increasing the ceftriaxone MIC; the presence of the <i>bla</i><sub>TEM</sub> plasmid, Gly120Asp, Ala121Gly/Asn substitutions in PorB, and the adenine deletion in the promoter region of the <i>mtrR</i> gene caused an increase in the penicillin MIC. Among 61 NG-MAST types identified, the most frequent were types 228, 807, 9486, 1993, and 6226. A link between <i>penA</i> alleles and <i>Neisseria gonorrhoeae</i> multi-antigen sequence typing (NG-MAST) types was established. Resistance to two groups of β-lactam antibiotics was associated with non-identical changes in <i>penA</i> alleles. To prevent the emergence of ceftriaxone resistance in Russia, NG-MAST genotyping must be supplemented with <i>penA</i> allele analysis.https://www.mdpi.com/2076-0817/9/11/941<i>Neisseria gonorrhoeae</i><i>penA</i> alleles<i>Neisseria gonorrhoeae</i> multi-antigen sequence typing (NG-MAST)drug resistancebenzylpenicillinceftriaxone |
| spellingShingle | Ilya Kandinov Ekaterina Dementieva Dmitry Kravtsov Alexander Chestkov Alexey Kubanov Victoria Solomka Dmitry Deryabin Dmitry Gryadunov Boris Shaskolskiy Molecular Typing of <i>Neisseria gonorrhoeae</i> Clinical Isolates in Russia, 2018–2019: A Link Between <i>penA</i> Alleles and NG-MAST Types Pathogens <i>Neisseria gonorrhoeae</i> <i>penA</i> alleles <i>Neisseria gonorrhoeae</i> multi-antigen sequence typing (NG-MAST) drug resistance benzylpenicillin ceftriaxone |
| title | Molecular Typing of <i>Neisseria gonorrhoeae</i> Clinical Isolates in Russia, 2018–2019: A Link Between <i>penA</i> Alleles and NG-MAST Types |
| title_full | Molecular Typing of <i>Neisseria gonorrhoeae</i> Clinical Isolates in Russia, 2018–2019: A Link Between <i>penA</i> Alleles and NG-MAST Types |
| title_fullStr | Molecular Typing of <i>Neisseria gonorrhoeae</i> Clinical Isolates in Russia, 2018–2019: A Link Between <i>penA</i> Alleles and NG-MAST Types |
| title_full_unstemmed | Molecular Typing of <i>Neisseria gonorrhoeae</i> Clinical Isolates in Russia, 2018–2019: A Link Between <i>penA</i> Alleles and NG-MAST Types |
| title_short | Molecular Typing of <i>Neisseria gonorrhoeae</i> Clinical Isolates in Russia, 2018–2019: A Link Between <i>penA</i> Alleles and NG-MAST Types |
| title_sort | molecular typing of i neisseria gonorrhoeae i clinical isolates in russia 2018 2019 a link between i pena i alleles and ng mast types |
| topic | <i>Neisseria gonorrhoeae</i> <i>penA</i> alleles <i>Neisseria gonorrhoeae</i> multi-antigen sequence typing (NG-MAST) drug resistance benzylpenicillin ceftriaxone |
| url | https://www.mdpi.com/2076-0817/9/11/941 |
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