Alterations of the Oral Microbiota Profiles in Chinese Patient With Oral Cancer
Oral cancer is the most common malignant tumor in the oral and maxillofacial region, of which more than 90% is squamous cell carcinoma. The incidence of oral cancer is on the rise worldwide. An imbalance between the microorganism composition and its host may lead to the occurrence of oral malignant...
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| Format: | Article |
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Frontiers Media S.A.
2021-12-01
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| Series: | Frontiers in Cellular and Infection Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcimb.2021.780067/full |
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| author | Zixuan Li Gang Chen Panpan Wang Minglei Sun Junfang Zhao Ang Li Qiang Sun |
| author_facet | Zixuan Li Gang Chen Panpan Wang Minglei Sun Junfang Zhao Ang Li Qiang Sun |
| author_sort | Zixuan Li |
| collection | DOAJ |
| description | Oral cancer is the most common malignant tumor in the oral and maxillofacial region, of which more than 90% is squamous cell carcinoma. The incidence of oral cancer is on the rise worldwide. An imbalance between the microorganism composition and its host may lead to the occurrence of oral malignant tumors. Accumulating evidence suggests that the oral microbiota plays an important role in oral cancer; however, the association between oral microbiota and oral cancer has not yet been comprehensively studied. In this study, metagenomic sequencing was used to compare the microbial composition of three groups of samples from Chinese patients with oral cancer, patients with precancerous lesion, and normal individuals. In terms of microbiota richness, the oral microbiota of patients with precancerous lesions was richer than that of oral cancer patients and healthy controls, whereas in terms of microbiota diversity, there was little difference between the three groups. The three groups of samples exhibited statistically significant differences in microbiota composition and metabolic function at the family, genus, and species levels (P < 0.05). The differentially enriched phylum in oral cancer samples was Bacteroidetes (P < 0.05). At the genus level, the main differentially enriched taxa were Prevotella, Peptostreptococcus, Carnobacterium, and Diastella (P < 0.05). The species level was differentially enriched in Prevotella intermedia and Peptostreptococcus stomatis (p < 0.05). The prediction of microbiota function shows that oral cancer is mainly associated with coenzyme A biosynthesis, phosphopantothenic acid biosynthesis, inosine 5’-phosphate degradation, and riboflavin biosynthesis. Furthermore, the increase in C-reactive protein level in oral cancer patients was found to be closely related to P. intermedia. Overall, oral bacterial profiles showed significant differences between the oral cancer group and normal group. Hence, microbes can be employed as diagnostic markers and treatment targets for oral cancer. |
| first_indexed | 2024-12-14T08:05:51Z |
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| language | English |
| last_indexed | 2024-12-14T08:05:51Z |
| publishDate | 2021-12-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj.art-01df40f6fc704bed9507b0781faaacb12022-12-21T23:10:11ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882021-12-011110.3389/fcimb.2021.780067780067Alterations of the Oral Microbiota Profiles in Chinese Patient With Oral CancerZixuan Li0Gang Chen1Panpan Wang2Minglei Sun3Junfang Zhao4Ang Li5Qiang Sun6Department of Oral and Maxillofacial Surgery, First Affiliated Hospital of Zhengzhou University, Zhengzhou, ChinaShenzhen Stomatology Hospital (Pingshan), Southern Medical University, Shenzhen, ChinaDepartment of Oral and Maxillofacial Surgery, First Affiliated Hospital of Zhengzhou University, Zhengzhou, ChinaDepartment of Oral and Maxillofacial Surgery, First Affiliated Hospital of Zhengzhou University, Zhengzhou, ChinaDepartment of Oral and Maxillofacial Surgery, First Affiliated Hospital of Zhengzhou University, Zhengzhou, ChinaHealth Management Center, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, ChinaDepartment of Oral and Maxillofacial Surgery, First Affiliated Hospital of Zhengzhou University, Zhengzhou, ChinaOral cancer is the most common malignant tumor in the oral and maxillofacial region, of which more than 90% is squamous cell carcinoma. The incidence of oral cancer is on the rise worldwide. An imbalance between the microorganism composition and its host may lead to the occurrence of oral malignant tumors. Accumulating evidence suggests that the oral microbiota plays an important role in oral cancer; however, the association between oral microbiota and oral cancer has not yet been comprehensively studied. In this study, metagenomic sequencing was used to compare the microbial composition of three groups of samples from Chinese patients with oral cancer, patients with precancerous lesion, and normal individuals. In terms of microbiota richness, the oral microbiota of patients with precancerous lesions was richer than that of oral cancer patients and healthy controls, whereas in terms of microbiota diversity, there was little difference between the three groups. The three groups of samples exhibited statistically significant differences in microbiota composition and metabolic function at the family, genus, and species levels (P < 0.05). The differentially enriched phylum in oral cancer samples was Bacteroidetes (P < 0.05). At the genus level, the main differentially enriched taxa were Prevotella, Peptostreptococcus, Carnobacterium, and Diastella (P < 0.05). The species level was differentially enriched in Prevotella intermedia and Peptostreptococcus stomatis (p < 0.05). The prediction of microbiota function shows that oral cancer is mainly associated with coenzyme A biosynthesis, phosphopantothenic acid biosynthesis, inosine 5’-phosphate degradation, and riboflavin biosynthesis. Furthermore, the increase in C-reactive protein level in oral cancer patients was found to be closely related to P. intermedia. Overall, oral bacterial profiles showed significant differences between the oral cancer group and normal group. Hence, microbes can be employed as diagnostic markers and treatment targets for oral cancer.https://www.frontiersin.org/articles/10.3389/fcimb.2021.780067/fulloral canceroral microbiotametagenomic sequencingcorrelationdiagnostic value |
| spellingShingle | Zixuan Li Gang Chen Panpan Wang Minglei Sun Junfang Zhao Ang Li Qiang Sun Alterations of the Oral Microbiota Profiles in Chinese Patient With Oral Cancer Frontiers in Cellular and Infection Microbiology oral cancer oral microbiota metagenomic sequencing correlation diagnostic value |
| title | Alterations of the Oral Microbiota Profiles in Chinese Patient With Oral Cancer |
| title_full | Alterations of the Oral Microbiota Profiles in Chinese Patient With Oral Cancer |
| title_fullStr | Alterations of the Oral Microbiota Profiles in Chinese Patient With Oral Cancer |
| title_full_unstemmed | Alterations of the Oral Microbiota Profiles in Chinese Patient With Oral Cancer |
| title_short | Alterations of the Oral Microbiota Profiles in Chinese Patient With Oral Cancer |
| title_sort | alterations of the oral microbiota profiles in chinese patient with oral cancer |
| topic | oral cancer oral microbiota metagenomic sequencing correlation diagnostic value |
| url | https://www.frontiersin.org/articles/10.3389/fcimb.2021.780067/full |
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