Analysis of microbial flora in skin lesions of primary acute guttate psoriasis
Objective To investigate the differences in the cutaneous microbiome between patients with primary acute guttate psoriasis and healthy controls. Methods We enrolled 18 patients with primary acute guttate psoriasis, including 9 patients with upper respiratory tract infection and 9 patients without up...
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| Format: | Article |
| Language: | zho |
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editoiral office of Journal of Diagnosis and Therapy on Dermato-venereology
2022-10-01
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| Series: | Pifu-xingbing zhenliaoxue zazhi |
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| Online Access: | http://pfxbzlx.gdvdc.com/CN/10.3969/j.issn.1674-8468.2022.05.002 |
| _version_ | 1797732626581159936 |
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| author | Jia ZHANG Kaiyun ZHANG Tao CHEN Juan ZHAO Hongmei WANG Yingfang XUE Guohui ZHANG |
| author_facet | Jia ZHANG Kaiyun ZHANG Tao CHEN Juan ZHAO Hongmei WANG Yingfang XUE Guohui ZHANG |
| author_sort | Jia ZHANG |
| collection | DOAJ |
| description | Objective To investigate the differences in the cutaneous microbiome between patients with primary acute guttate psoriasis and healthy controls. Methods We enrolled 18 patients with primary acute guttate psoriasis, including 9 patients with upper respiratory tract infection and 9 patients without upper respiratory tract infection, and 9 healthy controls. Skin swabs were collected from lesional and non-lesional skin of psoriatic patients and healthy controls. The 16S rDNA sequencing was used for analyzing the DNA samples and annotating the sequencing results. The differences in microbial species and community structure among the three groups were determined by bioinformatics and statistics analysis. Results At the Phylum level, the dominant species among the three groups were Firmicutes, Proteobacteria and Actinobacteria. There was no significant difference in α diversity analysis among the three groups (all P>0.05). There were significant differences in the composition of microbial community between the control group and the two groups of patients (both P<0.05). Gammaproteobacteria and Cutibacterium in the primary acute guttate psoriasis without upper respiratory tract infection were lower than those in the normal group (both P<0.05).Campylobacteria, Lawsonella and Actinomyces in the primary acute guttate psoriasis with upper respiratory tract infection were lower than those in the normal group (both P<0.05), but unidentified Weeksellaceae was the opposite (P<0.05). Gammaproteobacteria, Enhydrobacter and Pseudomonas in the primary acute guttate psoriasis without upper respiratory tract infection were lower than that in primary acute guttate psoriasis with upper respiratory tract infection (all P<0.05). Conclusions There are differences in the composition of microbial community among the three groups at the genus level. Upper respiratory tract infection affects the composition of microflora on the skin lesion of acute guttate psoriasis. The pathogenesis of psoriasis is associated with cutaneous microbiome. |
| first_indexed | 2024-03-12T12:16:17Z |
| format | Article |
| id | doaj.art-01e64b62e1494da8893fae4c09ce31fd |
| institution | Directory Open Access Journal |
| issn | 1674-8468 |
| language | zho |
| last_indexed | 2024-03-12T12:16:17Z |
| publishDate | 2022-10-01 |
| publisher | editoiral office of Journal of Diagnosis and Therapy on Dermato-venereology |
| record_format | Article |
| series | Pifu-xingbing zhenliaoxue zazhi |
| spelling | doaj.art-01e64b62e1494da8893fae4c09ce31fd2023-08-30T11:38:45Zzhoeditoiral office of Journal of Diagnosis and Therapy on Dermato-venereologyPifu-xingbing zhenliaoxue zazhi1674-84682022-10-0129539139810.3969/j.issn.1674-8468.2022.05.002Analysis of microbial flora in skin lesions of primary acute guttate psoriasisJia ZHANG0Kaiyun ZHANG1Tao CHEN2Juan ZHAO3Hongmei WANG4Yingfang XUE5Guohui ZHANG6The Central Hospital of BaotouThe Central Hospital of BaotouThe Central Hospital of BaotouThe Central Hospital of BaotouThe Central Hospital of BaotouThe Central Hospital of BaotouThe Central Hospital of BaotouObjective To investigate the differences in the cutaneous microbiome between patients with primary acute guttate psoriasis and healthy controls. Methods We enrolled 18 patients with primary acute guttate psoriasis, including 9 patients with upper respiratory tract infection and 9 patients without upper respiratory tract infection, and 9 healthy controls. Skin swabs were collected from lesional and non-lesional skin of psoriatic patients and healthy controls. The 16S rDNA sequencing was used for analyzing the DNA samples and annotating the sequencing results. The differences in microbial species and community structure among the three groups were determined by bioinformatics and statistics analysis. Results At the Phylum level, the dominant species among the three groups were Firmicutes, Proteobacteria and Actinobacteria. There was no significant difference in α diversity analysis among the three groups (all P>0.05). There were significant differences in the composition of microbial community between the control group and the two groups of patients (both P<0.05). Gammaproteobacteria and Cutibacterium in the primary acute guttate psoriasis without upper respiratory tract infection were lower than those in the normal group (both P<0.05).Campylobacteria, Lawsonella and Actinomyces in the primary acute guttate psoriasis with upper respiratory tract infection were lower than those in the normal group (both P<0.05), but unidentified Weeksellaceae was the opposite (P<0.05). Gammaproteobacteria, Enhydrobacter and Pseudomonas in the primary acute guttate psoriasis without upper respiratory tract infection were lower than that in primary acute guttate psoriasis with upper respiratory tract infection (all P<0.05). Conclusions There are differences in the composition of microbial community among the three groups at the genus level. Upper respiratory tract infection affects the composition of microflora on the skin lesion of acute guttate psoriasis. The pathogenesis of psoriasis is associated with cutaneous microbiome.http://pfxbzlx.gdvdc.com/CN/10.3969/j.issn.1674-8468.2022.05.002primary acute guttate psoriasismicrobial flora16s rdnaupper respiratory tract infection |
| spellingShingle | Jia ZHANG Kaiyun ZHANG Tao CHEN Juan ZHAO Hongmei WANG Yingfang XUE Guohui ZHANG Analysis of microbial flora in skin lesions of primary acute guttate psoriasis Pifu-xingbing zhenliaoxue zazhi primary acute guttate psoriasis microbial flora 16s rdna upper respiratory tract infection |
| title | Analysis of microbial flora in skin lesions of primary acute guttate psoriasis |
| title_full | Analysis of microbial flora in skin lesions of primary acute guttate psoriasis |
| title_fullStr | Analysis of microbial flora in skin lesions of primary acute guttate psoriasis |
| title_full_unstemmed | Analysis of microbial flora in skin lesions of primary acute guttate psoriasis |
| title_short | Analysis of microbial flora in skin lesions of primary acute guttate psoriasis |
| title_sort | analysis of microbial flora in skin lesions of primary acute guttate psoriasis |
| topic | primary acute guttate psoriasis microbial flora 16s rdna upper respiratory tract infection |
| url | http://pfxbzlx.gdvdc.com/CN/10.3969/j.issn.1674-8468.2022.05.002 |
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