Allosteric regulation of 6-phosphofructo-1-kinase activity of fat body and flight muscle from the bloodsucking bug Rhodnius prolixus
6-phosphofructo-1-kinase (phosphofructokinase; PFK) activity from Rhodnius prolixus, a haematophagous insect which is usually a poor flyer, was measured and compared in two metabolically active tissues - flight muscle and fat body. The activity of this important regulatory glycolytic enzyme was much...
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Academia Brasileira de Ciências
2007-03-01
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| Series: | Anais da Academia Brasileira de Ciências |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652007000100008 |
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| author | Gutemberg G. Alves Monica M. Marinho-Carvalho Georgia C. Atella Mario A.C. Silva-Neto Mauro Sola-Penna |
| author_facet | Gutemberg G. Alves Monica M. Marinho-Carvalho Georgia C. Atella Mario A.C. Silva-Neto Mauro Sola-Penna |
| author_sort | Gutemberg G. Alves |
| collection | DOAJ |
| description | 6-phosphofructo-1-kinase (phosphofructokinase; PFK) activity from Rhodnius prolixus, a haematophagous insect which is usually a poor flyer, was measured and compared in two metabolically active tissues - flight muscle and fat body. The activity of this important regulatory glycolytic enzyme was much more pronounced in muscle (15.1 ± 1.4 U/mg) than in fat body extracts (3.6±0.4 U/mg), although the latter presented higher levels of enzyme per protein content, as measured by western-blotting. Muscle extracts are more responsible than fat body to ATP and fructose 6-phosphate, both substrates of PFK. Allosteric regulation exerted by different effectors such as ADP, AMP and fructose 2,6-phosphate presented a singular pattern for each tissue. Optimal pH (8.0-8.5) and sensitivity to pH variation was very similar, and citrate was unable to inhibit PFK activity in both extracts. Our results suggest the existence of a particular PFK activity for each tissue, with regulatory patterns that are consistent with their physiological roles.<br>A atividade da fosfofrutocinase (PFK) de Rodnius prolixus, um inseto hematófago, o qual vôa somente pequenas distâncias, foi medida e comparada em dois tecidos metabolicamente ativos - músculo de asa e corpo gorduroso. A atividade desta importante enzima glicolítica regulatória foi muito mais pronunciada em músculo de asa (15,1 ±1,4 U/mg) do que em extrato de corpo gorduroso (3,6 ±0,4 U/mg) embora este último tenha apresentado níveis mais altos da enzima por quantidade de proteína, como medido por western-blotting. Extratos de músculo foram mais responsivos do que corpo gorduroso para ATP e frutose-6-fosfato, ambos substratos da PFK. A regulação alostérica exercida por diferentes efetores tais como ADP, AMP, frutose-2,6-bisfosfato apresentou um padrão singular para cada tecido. O pH ótimo (8,0-8,5) e a sensibilidade a variações de pH, foram muito similares e o citrato foi incapaz de inibir a atividade da PFK em ambos os extratos. Nossos resultados sugerem a existência de uma atividade particular da PFK para cada tecido com padrões regulatórios que são consistentes com suas funções fisiológicas. |
| first_indexed | 2024-04-11T22:50:24Z |
| format | Article |
| id | doaj.art-01e718d4547a45c8b968aaecf54756c2 |
| institution | Directory Open Access Journal |
| issn | 0001-3765 1678-2690 |
| language | English |
| last_indexed | 2024-04-11T22:50:24Z |
| publishDate | 2007-03-01 |
| publisher | Academia Brasileira de Ciências |
| record_format | Article |
| series | Anais da Academia Brasileira de Ciências |
| spelling | doaj.art-01e718d4547a45c8b968aaecf54756c22022-12-22T03:58:36ZengAcademia Brasileira de CiênciasAnais da Academia Brasileira de Ciências0001-37651678-26902007-03-01791536210.1590/S0001-37652007000100008Allosteric regulation of 6-phosphofructo-1-kinase activity of fat body and flight muscle from the bloodsucking bug Rhodnius prolixusGutemberg G. AlvesMonica M. Marinho-CarvalhoGeorgia C. AtellaMario A.C. Silva-NetoMauro Sola-Penna6-phosphofructo-1-kinase (phosphofructokinase; PFK) activity from Rhodnius prolixus, a haematophagous insect which is usually a poor flyer, was measured and compared in two metabolically active tissues - flight muscle and fat body. The activity of this important regulatory glycolytic enzyme was much more pronounced in muscle (15.1 ± 1.4 U/mg) than in fat body extracts (3.6±0.4 U/mg), although the latter presented higher levels of enzyme per protein content, as measured by western-blotting. Muscle extracts are more responsible than fat body to ATP and fructose 6-phosphate, both substrates of PFK. Allosteric regulation exerted by different effectors such as ADP, AMP and fructose 2,6-phosphate presented a singular pattern for each tissue. Optimal pH (8.0-8.5) and sensitivity to pH variation was very similar, and citrate was unable to inhibit PFK activity in both extracts. Our results suggest the existence of a particular PFK activity for each tissue, with regulatory patterns that are consistent with their physiological roles.<br>A atividade da fosfofrutocinase (PFK) de Rodnius prolixus, um inseto hematófago, o qual vôa somente pequenas distâncias, foi medida e comparada em dois tecidos metabolicamente ativos - músculo de asa e corpo gorduroso. A atividade desta importante enzima glicolítica regulatória foi muito mais pronunciada em músculo de asa (15,1 ±1,4 U/mg) do que em extrato de corpo gorduroso (3,6 ±0,4 U/mg) embora este último tenha apresentado níveis mais altos da enzima por quantidade de proteína, como medido por western-blotting. Extratos de músculo foram mais responsivos do que corpo gorduroso para ATP e frutose-6-fosfato, ambos substratos da PFK. A regulação alostérica exercida por diferentes efetores tais como ADP, AMP, frutose-2,6-bisfosfato apresentou um padrão singular para cada tecido. O pH ótimo (8,0-8,5) e a sensibilidade a variações de pH, foram muito similares e o citrato foi incapaz de inibir a atividade da PFK em ambos os extratos. Nossos resultados sugerem a existência de uma atividade particular da PFK para cada tecido com padrões regulatórios que são consistentes com suas funções fisiológicas.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652007000100008fosfotrutocinasemetabolismoinsetoglicólisemúsculocorpo gordurosophosphofructokinasemetabolisminsect |
| spellingShingle | Gutemberg G. Alves Monica M. Marinho-Carvalho Georgia C. Atella Mario A.C. Silva-Neto Mauro Sola-Penna Allosteric regulation of 6-phosphofructo-1-kinase activity of fat body and flight muscle from the bloodsucking bug Rhodnius prolixus Anais da Academia Brasileira de Ciências fosfotrutocinase metabolismo inseto glicólise músculo corpo gorduroso phosphofructokinase metabolism insect |
| title | Allosteric regulation of 6-phosphofructo-1-kinase activity of fat body and flight muscle from the bloodsucking bug Rhodnius prolixus |
| title_full | Allosteric regulation of 6-phosphofructo-1-kinase activity of fat body and flight muscle from the bloodsucking bug Rhodnius prolixus |
| title_fullStr | Allosteric regulation of 6-phosphofructo-1-kinase activity of fat body and flight muscle from the bloodsucking bug Rhodnius prolixus |
| title_full_unstemmed | Allosteric regulation of 6-phosphofructo-1-kinase activity of fat body and flight muscle from the bloodsucking bug Rhodnius prolixus |
| title_short | Allosteric regulation of 6-phosphofructo-1-kinase activity of fat body and flight muscle from the bloodsucking bug Rhodnius prolixus |
| title_sort | allosteric regulation of 6 phosphofructo 1 kinase activity of fat body and flight muscle from the bloodsucking bug rhodnius prolixus |
| topic | fosfotrutocinase metabolismo inseto glicólise músculo corpo gorduroso phosphofructokinase metabolism insect |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652007000100008 |
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