ATG13 is involved in immune response of pathogen invasion in blood clam Tegillarca granosa
Mammalian autophagy-related gene 13 (ATG13) is a vital component of the ATG1 autophagy initiation complex which plays an essential role in autophagy. However, the molecular function of ATG13 in pathogen defense in invertebrates is still poorly understood. In this study, the full-length cDNA sequence...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-03-01
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| Series: | Frontiers in Veterinary Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fvets.2023.1141284/full |
| _version_ | 1811161665030324224 |
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| author | Yuling Wu Yuling Wu Guosheng Liu Zengpeng Li Mingliang Chen Mingliang Chen Mingliang Chen Qin Wang |
| author_facet | Yuling Wu Yuling Wu Guosheng Liu Zengpeng Li Mingliang Chen Mingliang Chen Mingliang Chen Qin Wang |
| author_sort | Yuling Wu |
| collection | DOAJ |
| description | Mammalian autophagy-related gene 13 (ATG13) is a vital component of the ATG1 autophagy initiation complex which plays an essential role in autophagy. However, the molecular function of ATG13 in pathogen defense in invertebrates is still poorly understood. In this study, the full-length cDNA sequence of blood clam Tegillarca granosa ATG13 (TgATG13) was obtained, which was 1,918 bp in length, including 283 bp 5' UTR, 252 bp 3' UTR and 1,383 bp open reading frame (ORF) encoding 460 amino acids. Phylogenetic analysis revealed that TgATG13 had the closest relationship with that of Crassostrea Virginica. Quantitative real-time PCR results showed that the transcript of TgATG13 was universally expressed in various tissues of blood clam, with the highest expression level in hemocytes. The expression level of TgATG13 was robustly increased after exposure of both Vibrio alginolyticus and LPS. Fluorescence confocal microscopy further showed that TgATG13 promoted the production of autophagosome. In summary, our study demonstrated that TgATG13 was involved in the immune regulation of blood clam during pathogen invasion, deepening our understanding of the innate immune mechanism of blood clam. |
| first_indexed | 2024-04-10T06:17:59Z |
| format | Article |
| id | doaj.art-01e89cb4963b49f4a0aee64dc7886feb |
| institution | Directory Open Access Journal |
| issn | 2297-1769 |
| language | English |
| last_indexed | 2024-04-10T06:17:59Z |
| publishDate | 2023-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Veterinary Science |
| spelling | doaj.art-01e89cb4963b49f4a0aee64dc7886feb2023-03-02T05:29:52ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692023-03-011010.3389/fvets.2023.11412841141284ATG13 is involved in immune response of pathogen invasion in blood clam Tegillarca granosaYuling Wu0Yuling Wu1Guosheng Liu2Zengpeng Li3Mingliang Chen4Mingliang Chen5Mingliang Chen6Qin Wang7State Key Laboratory Breeding Base of Marine Genetic Resources, Third Institute of Oceanography, Ministry of Natural Resources, Xiamen, ChinaSchool of Life Science, Xiamen University, Xiamen, ChinaState Key Laboratory Breeding Base of Marine Genetic Resources, Third Institute of Oceanography, Ministry of Natural Resources, Xiamen, ChinaState Key Laboratory Breeding Base of Marine Genetic Resources, Third Institute of Oceanography, Ministry of Natural Resources, Xiamen, ChinaState Key Laboratory Breeding Base of Marine Genetic Resources, Third Institute of Oceanography, Ministry of Natural Resources, Xiamen, ChinaCo-innovation Center of Jiangsu Marine Bio-Industry Technology, Jiangsu Ocean University, Lianyungang, ChinaSchool of Marine Biology, Xiamen Ocean Vocational College, Xiamen, ChinaSchool of Life Science, Xiamen University, Xiamen, ChinaMammalian autophagy-related gene 13 (ATG13) is a vital component of the ATG1 autophagy initiation complex which plays an essential role in autophagy. However, the molecular function of ATG13 in pathogen defense in invertebrates is still poorly understood. In this study, the full-length cDNA sequence of blood clam Tegillarca granosa ATG13 (TgATG13) was obtained, which was 1,918 bp in length, including 283 bp 5' UTR, 252 bp 3' UTR and 1,383 bp open reading frame (ORF) encoding 460 amino acids. Phylogenetic analysis revealed that TgATG13 had the closest relationship with that of Crassostrea Virginica. Quantitative real-time PCR results showed that the transcript of TgATG13 was universally expressed in various tissues of blood clam, with the highest expression level in hemocytes. The expression level of TgATG13 was robustly increased after exposure of both Vibrio alginolyticus and LPS. Fluorescence confocal microscopy further showed that TgATG13 promoted the production of autophagosome. In summary, our study demonstrated that TgATG13 was involved in the immune regulation of blood clam during pathogen invasion, deepening our understanding of the innate immune mechanism of blood clam.https://www.frontiersin.org/articles/10.3389/fvets.2023.1141284/fullTegillarca granosaATG13autophagyvibrio infectionLPS |
| spellingShingle | Yuling Wu Yuling Wu Guosheng Liu Zengpeng Li Mingliang Chen Mingliang Chen Mingliang Chen Qin Wang ATG13 is involved in immune response of pathogen invasion in blood clam Tegillarca granosa Frontiers in Veterinary Science Tegillarca granosa ATG13 autophagy vibrio infection LPS |
| title | ATG13 is involved in immune response of pathogen invasion in blood clam Tegillarca granosa |
| title_full | ATG13 is involved in immune response of pathogen invasion in blood clam Tegillarca granosa |
| title_fullStr | ATG13 is involved in immune response of pathogen invasion in blood clam Tegillarca granosa |
| title_full_unstemmed | ATG13 is involved in immune response of pathogen invasion in blood clam Tegillarca granosa |
| title_short | ATG13 is involved in immune response of pathogen invasion in blood clam Tegillarca granosa |
| title_sort | atg13 is involved in immune response of pathogen invasion in blood clam tegillarca granosa |
| topic | Tegillarca granosa ATG13 autophagy vibrio infection LPS |
| url | https://www.frontiersin.org/articles/10.3389/fvets.2023.1141284/full |
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