Effects of β-1,6-Glucan Synthase Gene (<i>FfGS6</i>) Overexpression on Stress Response and Fruit Body Development in <i>Flammulina filiformis</i>
β-1, 6-glucan synthase is a key enzyme of β-1, 6-glucan synthesis, which plays a vital role in the cell wall cross-linking of fungi. However, the role of the β-1, 6-glucan synthase gene in the development of the fruiting body and the stress response of macrofungi is largely unknown. In this study, f...
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MDPI AG
2022-09-01
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| author | Yuanyuan Liu Xinbin Ma Ying Long Sen Yao Chuanzheng Wei Xing Han Bingcheng Gan Junjie Yan Baogui Xie |
| author_facet | Yuanyuan Liu Xinbin Ma Ying Long Sen Yao Chuanzheng Wei Xing Han Bingcheng Gan Junjie Yan Baogui Xie |
| author_sort | Yuanyuan Liu |
| collection | DOAJ |
| description | β-1, 6-glucan synthase is a key enzyme of β-1, 6-glucan synthesis, which plays a vital role in the cell wall cross-linking of fungi. However, the role of the β-1, 6-glucan synthase gene in the development of the fruiting body and the stress response of macrofungi is largely unknown. In this study, four overexpression transformants of the β-1, 6-glucan synthase gene (<i>FfGS6</i>) were successfully obtained, and gene function was studied in <i>Flammulina filiformis</i>. The overexpression of <i>FfGS6</i> can increase the width of mycelium cells and improve the tolerance ability under mechanical injury and oxidative stress. Moreover, <i>FfGS6</i> gene expression fluctuated in up-regulation during the recovery process of mycelium injury but showed a negative correlation with H<sub>2</sub>O<sub>2</sub> concentration. Fruiting body phenotype tests showed that mycelia’s recovery ability after scratching improved when the <i>FfGS6</i> gene was overexpressed. However, primordia formation and the stipe elongation ability were significantly inhibited. Our findings indicate that <i>FfGS6</i> is involved in regulating mycelial cell morphology, the mycelial stress response, and fruit body development in <i>F. filiformis</i>. |
| first_indexed | 2024-03-09T20:10:16Z |
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| issn | 2073-4425 |
| language | English |
| last_indexed | 2024-03-09T20:10:16Z |
| publishDate | 2022-09-01 |
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| spelling | doaj.art-01ea8c0a96714d9ba4c72b0a1c17f7d92023-11-24T00:15:16ZengMDPI AGGenes2073-44252022-09-011310175310.3390/genes13101753Effects of β-1,6-Glucan Synthase Gene (<i>FfGS6</i>) Overexpression on Stress Response and Fruit Body Development in <i>Flammulina filiformis</i>Yuanyuan Liu0Xinbin Ma1Ying Long2Sen Yao3Chuanzheng Wei4Xing Han5Bingcheng Gan6Junjie Yan7Baogui Xie8Mycological Research Center, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaMycological Research Center, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaMycological Research Center, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaMycological Research Center, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaMycological Research Center, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaInstitute of Urban Agriculture, Chinese Academy of Agricultural Sciences, Chengdu 610213, ChinaInstitute of Urban Agriculture, Chinese Academy of Agricultural Sciences, Chengdu 610213, ChinaInstitute of Urban Agriculture, Chinese Academy of Agricultural Sciences, Chengdu 610213, ChinaMycological Research Center, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, Chinaβ-1, 6-glucan synthase is a key enzyme of β-1, 6-glucan synthesis, which plays a vital role in the cell wall cross-linking of fungi. However, the role of the β-1, 6-glucan synthase gene in the development of the fruiting body and the stress response of macrofungi is largely unknown. In this study, four overexpression transformants of the β-1, 6-glucan synthase gene (<i>FfGS6</i>) were successfully obtained, and gene function was studied in <i>Flammulina filiformis</i>. The overexpression of <i>FfGS6</i> can increase the width of mycelium cells and improve the tolerance ability under mechanical injury and oxidative stress. Moreover, <i>FfGS6</i> gene expression fluctuated in up-regulation during the recovery process of mycelium injury but showed a negative correlation with H<sub>2</sub>O<sub>2</sub> concentration. Fruiting body phenotype tests showed that mycelia’s recovery ability after scratching improved when the <i>FfGS6</i> gene was overexpressed. However, primordia formation and the stipe elongation ability were significantly inhibited. Our findings indicate that <i>FfGS6</i> is involved in regulating mycelial cell morphology, the mycelial stress response, and fruit body development in <i>F. filiformis</i>.https://www.mdpi.com/2073-4425/13/10/1753<i>F. filiformis</i>β-1,6-glucan synthaseoxidative stressinjury stressstipe elongation |
| spellingShingle | Yuanyuan Liu Xinbin Ma Ying Long Sen Yao Chuanzheng Wei Xing Han Bingcheng Gan Junjie Yan Baogui Xie Effects of β-1,6-Glucan Synthase Gene (<i>FfGS6</i>) Overexpression on Stress Response and Fruit Body Development in <i>Flammulina filiformis</i> Genes <i>F. filiformis</i> β-1,6-glucan synthase oxidative stress injury stress stipe elongation |
| title | Effects of β-1,6-Glucan Synthase Gene (<i>FfGS6</i>) Overexpression on Stress Response and Fruit Body Development in <i>Flammulina filiformis</i> |
| title_full | Effects of β-1,6-Glucan Synthase Gene (<i>FfGS6</i>) Overexpression on Stress Response and Fruit Body Development in <i>Flammulina filiformis</i> |
| title_fullStr | Effects of β-1,6-Glucan Synthase Gene (<i>FfGS6</i>) Overexpression on Stress Response and Fruit Body Development in <i>Flammulina filiformis</i> |
| title_full_unstemmed | Effects of β-1,6-Glucan Synthase Gene (<i>FfGS6</i>) Overexpression on Stress Response and Fruit Body Development in <i>Flammulina filiformis</i> |
| title_short | Effects of β-1,6-Glucan Synthase Gene (<i>FfGS6</i>) Overexpression on Stress Response and Fruit Body Development in <i>Flammulina filiformis</i> |
| title_sort | effects of β 1 6 glucan synthase gene i ffgs6 i overexpression on stress response and fruit body development in i flammulina filiformis i |
| topic | <i>F. filiformis</i> β-1,6-glucan synthase oxidative stress injury stress stipe elongation |
| url | https://www.mdpi.com/2073-4425/13/10/1753 |
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