Muscle Enriched Lamin Interacting Protein (<i>Mlip</i>) Binds Chromatin and Is Required for Myoblast Differentiation
Muscle-enriched A-type lamin-interacting protein (<i>Mlip</i>) is a recently discovered Amniota gene that encodes proteins of unknown biological function. Here we report <i>Mlip</i>’s direct interaction with chromatin, and it may function as a transcriptional co-factor. Chrom...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-03-01
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| Series: | Cells |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2073-4409/10/3/615 |
| Summary: | Muscle-enriched A-type lamin-interacting protein (<i>Mlip</i>) is a recently discovered Amniota gene that encodes proteins of unknown biological function. Here we report <i>Mlip</i>’s direct interaction with chromatin, and it may function as a transcriptional co-factor. Chromatin immunoprecipitations with microarray analysis demonstrated a propensity for <i>Mlip</i> to associate with genomic regions in close proximity to genes that control tissue-specific differentiation. Gel mobility shift assays confirmed that <i>Mlip</i> protein complexes with genomic DNA. Blocking <i>Mlip</i> expression in C2C12 myoblasts down-regulates myogenic regulatory factors (MyoD and MyoG) and subsequently significantly inhibits myogenic differentiation and the formation of myotubes. Collectively our data demonstrate that <i>Mlip</i> is required for C2C12 myoblast differentiation into myotubes. <i>Mlip</i> may exert this role as a transcriptional regulator of a myogenic program that is unique to amniotes. |
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| ISSN: | 2073-4409 |