Muscle Enriched Lamin Interacting Protein (<i>Mlip</i>) Binds Chromatin and Is Required for Myoblast Differentiation
Muscle-enriched A-type lamin-interacting protein (<i>Mlip</i>) is a recently discovered Amniota gene that encodes proteins of unknown biological function. Here we report <i>Mlip</i>’s direct interaction with chromatin, and it may function as a transcriptional co-factor. Chrom...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-03-01
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| Series: | Cells |
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| Online Access: | https://www.mdpi.com/2073-4409/10/3/615 |
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| author | Elmira Ahmady Alexandre Blais Patrick G. Burgon |
| author_facet | Elmira Ahmady Alexandre Blais Patrick G. Burgon |
| author_sort | Elmira Ahmady |
| collection | DOAJ |
| description | Muscle-enriched A-type lamin-interacting protein (<i>Mlip</i>) is a recently discovered Amniota gene that encodes proteins of unknown biological function. Here we report <i>Mlip</i>’s direct interaction with chromatin, and it may function as a transcriptional co-factor. Chromatin immunoprecipitations with microarray analysis demonstrated a propensity for <i>Mlip</i> to associate with genomic regions in close proximity to genes that control tissue-specific differentiation. Gel mobility shift assays confirmed that <i>Mlip</i> protein complexes with genomic DNA. Blocking <i>Mlip</i> expression in C2C12 myoblasts down-regulates myogenic regulatory factors (MyoD and MyoG) and subsequently significantly inhibits myogenic differentiation and the formation of myotubes. Collectively our data demonstrate that <i>Mlip</i> is required for C2C12 myoblast differentiation into myotubes. <i>Mlip</i> may exert this role as a transcriptional regulator of a myogenic program that is unique to amniotes. |
| first_indexed | 2024-03-10T13:20:53Z |
| format | Article |
| id | doaj.art-01f2641372e141b1833687705a15928f |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-10T13:20:53Z |
| publishDate | 2021-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-01f2641372e141b1833687705a15928f2023-11-21T09:59:24ZengMDPI AGCells2073-44092021-03-0110361510.3390/cells10030615Muscle Enriched Lamin Interacting Protein (<i>Mlip</i>) Binds Chromatin and Is Required for Myoblast DifferentiationElmira Ahmady0Alexandre Blais1Patrick G. Burgon2Department of Biochemistry, Microbiology, and Immunology, University of Ottawa, Ottawa, ON K1H 8M5, CanadaDepartment of Biochemistry, Microbiology, and Immunology, University of Ottawa, Ottawa, ON K1H 8M5, CanadaDepartment of Chemistry and Earth Sciences, College of Arts and Sciences, Qatar University, Doha 999043, QatarMuscle-enriched A-type lamin-interacting protein (<i>Mlip</i>) is a recently discovered Amniota gene that encodes proteins of unknown biological function. Here we report <i>Mlip</i>’s direct interaction with chromatin, and it may function as a transcriptional co-factor. Chromatin immunoprecipitations with microarray analysis demonstrated a propensity for <i>Mlip</i> to associate with genomic regions in close proximity to genes that control tissue-specific differentiation. Gel mobility shift assays confirmed that <i>Mlip</i> protein complexes with genomic DNA. Blocking <i>Mlip</i> expression in C2C12 myoblasts down-regulates myogenic regulatory factors (MyoD and MyoG) and subsequently significantly inhibits myogenic differentiation and the formation of myotubes. Collectively our data demonstrate that <i>Mlip</i> is required for C2C12 myoblast differentiation into myotubes. <i>Mlip</i> may exert this role as a transcriptional regulator of a myogenic program that is unique to amniotes.https://www.mdpi.com/2073-4409/10/3/615<i>Mlip</i>ChIPskeletal muscle |
| spellingShingle | Elmira Ahmady Alexandre Blais Patrick G. Burgon Muscle Enriched Lamin Interacting Protein (<i>Mlip</i>) Binds Chromatin and Is Required for Myoblast Differentiation Cells <i>Mlip</i> ChIP skeletal muscle |
| title | Muscle Enriched Lamin Interacting Protein (<i>Mlip</i>) Binds Chromatin and Is Required for Myoblast Differentiation |
| title_full | Muscle Enriched Lamin Interacting Protein (<i>Mlip</i>) Binds Chromatin and Is Required for Myoblast Differentiation |
| title_fullStr | Muscle Enriched Lamin Interacting Protein (<i>Mlip</i>) Binds Chromatin and Is Required for Myoblast Differentiation |
| title_full_unstemmed | Muscle Enriched Lamin Interacting Protein (<i>Mlip</i>) Binds Chromatin and Is Required for Myoblast Differentiation |
| title_short | Muscle Enriched Lamin Interacting Protein (<i>Mlip</i>) Binds Chromatin and Is Required for Myoblast Differentiation |
| title_sort | muscle enriched lamin interacting protein i mlip i binds chromatin and is required for myoblast differentiation |
| topic | <i>Mlip</i> ChIP skeletal muscle |
| url | https://www.mdpi.com/2073-4409/10/3/615 |
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