Fine mapping of the flavonoid 3’,5’-hydroxylase gene controlling anthocyanin biosynthesis in pepper anthers and stems
Pepper (Capsicum annuum L) is one of the most important vegetables grown worldwide. Nevertheless, the key structural and regulatory genes involved in anthocyanin accumulation in pepper have not been well understood or fine mapped yet. In this study, F1, F2, BC1P1, and BC1P2 pepper populations were a...
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Frontiers Media S.A.
2023-07-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2023.1232755/full |
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| author | Yixin Wang Yixin Wang Zheng Wang Heshan Du Bin Chen Guoyun Wang Qian Wang Sansheng Geng Xiaofen Zhang |
| author_facet | Yixin Wang Yixin Wang Zheng Wang Heshan Du Bin Chen Guoyun Wang Qian Wang Sansheng Geng Xiaofen Zhang |
| author_sort | Yixin Wang |
| collection | DOAJ |
| description | Pepper (Capsicum annuum L) is one of the most important vegetables grown worldwide. Nevertheless, the key structural and regulatory genes involved in anthocyanin accumulation in pepper have not been well understood or fine mapped yet. In this study, F1, F2, BC1P1, and BC1P2 pepper populations were analyzed and these populations were derived from a cross between line 14-Z4, which has yellow anthers and green stems, and line 14-Z5, which has purple anthers and stems. The results showed that the yellow anthers and green stems were determined by a single recessive locus called to as ayw. While, using preliminary and fine mapping techniques, ayw locus was located between markers aywSNP120 and aywSNP124, with physical distance of 0.2 Mb. The CA11g18550 gene was identified as promising candidate for the ayw locus, as it co-segregated with the yellow anthers and green stems phenotypes. CA11g18550 encodes a homolog of the F3’5’H (flavonoid 3’,5’-hydroxylase) anthocyanin synthesis structure gene. The missense mutation of CA11g18550 possibly resulted in a loss-of-function. The expression analysis showed that CA11g18550 was significantly expressed in the stems, leaves, anthers and petals in 14-Z5, and it’s silencing caused the stems changing from purple to green. This study provides a theoretical basis for using yellow anthers and green stems in pepper breeding and helps to advance the understanding of anthocyanin synthesis. |
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| id | doaj.art-01f428cde1564fc5a99b8bfc9bea3264 |
| institution | Directory Open Access Journal |
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| last_indexed | 2024-03-12T21:32:08Z |
| publishDate | 2023-07-01 |
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| series | Frontiers in Plant Science |
| spelling | doaj.art-01f428cde1564fc5a99b8bfc9bea32642023-07-27T17:17:35ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2023-07-011410.3389/fpls.2023.12327551232755Fine mapping of the flavonoid 3’,5’-hydroxylase gene controlling anthocyanin biosynthesis in pepper anthers and stemsYixin Wang0Yixin Wang1Zheng Wang2Heshan Du3Bin Chen4Guoyun Wang5Qian Wang6Sansheng Geng7Xiaofen Zhang8Beijing Key Laboratory of Vegetable Germplasms Improvement, Key Laboratory of Biology and Genetics Improvement of Horticultural Crops (North China), National Engineering Research Center for Vegetables, State Key Laboratory of Vegetable Biobreeding, Beijing Vegetable Research Center, Beijing Academy of Agriculture and Forestry Sciences, Beijing, ChinaDepartment of Vegetable Science, College of Horticulture, China Agricultural University, Beijing, ChinaBeijing Key Laboratory of Vegetable Germplasms Improvement, Key Laboratory of Biology and Genetics Improvement of Horticultural Crops (North China), National Engineering Research Center for Vegetables, State Key Laboratory of Vegetable Biobreeding, Beijing Vegetable Research Center, Beijing Academy of Agriculture and Forestry Sciences, Beijing, ChinaBeijing Key Laboratory of Vegetable Germplasms Improvement, Key Laboratory of Biology and Genetics Improvement of Horticultural Crops (North China), National Engineering Research Center for Vegetables, State Key Laboratory of Vegetable Biobreeding, Beijing Vegetable Research Center, Beijing Academy of Agriculture and Forestry Sciences, Beijing, ChinaBeijing Key Laboratory of Vegetable Germplasms Improvement, Key Laboratory of Biology and Genetics Improvement of Horticultural Crops (North China), National Engineering Research Center for Vegetables, State Key Laboratory of Vegetable Biobreeding, Beijing Vegetable Research Center, Beijing Academy of Agriculture and Forestry Sciences, Beijing, ChinaBeijing Key Laboratory of Vegetable Germplasms Improvement, Key Laboratory of Biology and Genetics Improvement of Horticultural Crops (North China), National Engineering Research Center for Vegetables, State Key Laboratory of Vegetable Biobreeding, Beijing Vegetable Research Center, Beijing Academy of Agriculture and Forestry Sciences, Beijing, ChinaDepartment of Vegetable Science, College of Horticulture, China Agricultural University, Beijing, ChinaBeijing Key Laboratory of Vegetable Germplasms Improvement, Key Laboratory of Biology and Genetics Improvement of Horticultural Crops (North China), National Engineering Research Center for Vegetables, State Key Laboratory of Vegetable Biobreeding, Beijing Vegetable Research Center, Beijing Academy of Agriculture and Forestry Sciences, Beijing, ChinaBeijing Key Laboratory of Vegetable Germplasms Improvement, Key Laboratory of Biology and Genetics Improvement of Horticultural Crops (North China), National Engineering Research Center for Vegetables, State Key Laboratory of Vegetable Biobreeding, Beijing Vegetable Research Center, Beijing Academy of Agriculture and Forestry Sciences, Beijing, ChinaPepper (Capsicum annuum L) is one of the most important vegetables grown worldwide. Nevertheless, the key structural and regulatory genes involved in anthocyanin accumulation in pepper have not been well understood or fine mapped yet. In this study, F1, F2, BC1P1, and BC1P2 pepper populations were analyzed and these populations were derived from a cross between line 14-Z4, which has yellow anthers and green stems, and line 14-Z5, which has purple anthers and stems. The results showed that the yellow anthers and green stems were determined by a single recessive locus called to as ayw. While, using preliminary and fine mapping techniques, ayw locus was located between markers aywSNP120 and aywSNP124, with physical distance of 0.2 Mb. The CA11g18550 gene was identified as promising candidate for the ayw locus, as it co-segregated with the yellow anthers and green stems phenotypes. CA11g18550 encodes a homolog of the F3’5’H (flavonoid 3’,5’-hydroxylase) anthocyanin synthesis structure gene. The missense mutation of CA11g18550 possibly resulted in a loss-of-function. The expression analysis showed that CA11g18550 was significantly expressed in the stems, leaves, anthers and petals in 14-Z5, and it’s silencing caused the stems changing from purple to green. This study provides a theoretical basis for using yellow anthers and green stems in pepper breeding and helps to advance the understanding of anthocyanin synthesis.https://www.frontiersin.org/articles/10.3389/fpls.2023.1232755/fullCapsicum annuum LanthocyaninsdelphinidinaywF3’5’HCA11g18550 |
| spellingShingle | Yixin Wang Yixin Wang Zheng Wang Heshan Du Bin Chen Guoyun Wang Qian Wang Sansheng Geng Xiaofen Zhang Fine mapping of the flavonoid 3’,5’-hydroxylase gene controlling anthocyanin biosynthesis in pepper anthers and stems Frontiers in Plant Science Capsicum annuum L anthocyanins delphinidin ayw F3’5’H CA11g18550 |
| title | Fine mapping of the flavonoid 3’,5’-hydroxylase gene controlling anthocyanin biosynthesis in pepper anthers and stems |
| title_full | Fine mapping of the flavonoid 3’,5’-hydroxylase gene controlling anthocyanin biosynthesis in pepper anthers and stems |
| title_fullStr | Fine mapping of the flavonoid 3’,5’-hydroxylase gene controlling anthocyanin biosynthesis in pepper anthers and stems |
| title_full_unstemmed | Fine mapping of the flavonoid 3’,5’-hydroxylase gene controlling anthocyanin biosynthesis in pepper anthers and stems |
| title_short | Fine mapping of the flavonoid 3’,5’-hydroxylase gene controlling anthocyanin biosynthesis in pepper anthers and stems |
| title_sort | fine mapping of the flavonoid 3 5 hydroxylase gene controlling anthocyanin biosynthesis in pepper anthers and stems |
| topic | Capsicum annuum L anthocyanins delphinidin ayw F3’5’H CA11g18550 |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2023.1232755/full |
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