Isobutanol production in Synechocystis PCC 6803 using heterologous and endogenous alcohol dehydrogenases
Isobutanol is a flammable compound that can be used as a biofuel due to its high energy density and suitable physical and chemical properties. In this study, we examined the capacity of engineered strains of Synechocystis PCC 6803 containing the α-ketoisovalerate decarboxylase from Lactococcus lacti...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2017-12-01
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| Series: | Metabolic Engineering Communications |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2214030117300123 |
| _version_ | 1818540636940271616 |
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| author | Rui Miao Xufeng Liu Elias Englund Pia Lindberg Peter Lindblad |
| author_facet | Rui Miao Xufeng Liu Elias Englund Pia Lindberg Peter Lindblad |
| author_sort | Rui Miao |
| collection | DOAJ |
| description | Isobutanol is a flammable compound that can be used as a biofuel due to its high energy density and suitable physical and chemical properties. In this study, we examined the capacity of engineered strains of Synechocystis PCC 6803 containing the α-ketoisovalerate decarboxylase from Lactococcus lactis and different heterologous and endogenous alcohol dehydrogenases (ADH) for isobutanol production. A strain expressing an introduced kivd without any additional copy of ADH produced 3 mg L−1 OD750−1 isobutanol in 6 days. After the cultures were supplemented with external addition of isobutyraldehyde, the substrate for ADH, 60.8 mg L−1 isobutanol was produced after 24 h when OD750 was 0.8. The in vivo activities of four different ADHs, two heterologous and two putative endogenous in Synechocystis, were examined and the Synechocystis endogenous ADH encoded by slr1192 showed the highest efficiency for isobutanol production. Furthermore, the strain overexpressing the isobutanol pathway on a self-replicating vector with the strong Ptrc promoter showed significantly higher gene expression and isobutanol production compared to the corresponding strains expressing the same operon introduced on the genome. Hence, this study demonstrates that Synechocystis endogenous AHDs have a high capacity for isobutanol production, and identifies kivd encoded α-ketoisovalerate decarboxylase as one of the likely bottlenecks for further isobutanol production. |
| first_indexed | 2024-12-11T21:57:59Z |
| format | Article |
| id | doaj.art-01f7a521721348a7803cbecdc465da7f |
| institution | Directory Open Access Journal |
| issn | 2214-0301 |
| language | English |
| last_indexed | 2024-12-11T21:57:59Z |
| publishDate | 2017-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Metabolic Engineering Communications |
| spelling | doaj.art-01f7a521721348a7803cbecdc465da7f2022-12-22T00:49:14ZengElsevierMetabolic Engineering Communications2214-03012017-12-015C455310.1016/j.meteno.2017.07.003Isobutanol production in Synechocystis PCC 6803 using heterologous and endogenous alcohol dehydrogenasesRui MiaoXufeng LiuElias EnglundPia LindbergPeter LindbladIsobutanol is a flammable compound that can be used as a biofuel due to its high energy density and suitable physical and chemical properties. In this study, we examined the capacity of engineered strains of Synechocystis PCC 6803 containing the α-ketoisovalerate decarboxylase from Lactococcus lactis and different heterologous and endogenous alcohol dehydrogenases (ADH) for isobutanol production. A strain expressing an introduced kivd without any additional copy of ADH produced 3 mg L−1 OD750−1 isobutanol in 6 days. After the cultures were supplemented with external addition of isobutyraldehyde, the substrate for ADH, 60.8 mg L−1 isobutanol was produced after 24 h when OD750 was 0.8. The in vivo activities of four different ADHs, two heterologous and two putative endogenous in Synechocystis, were examined and the Synechocystis endogenous ADH encoded by slr1192 showed the highest efficiency for isobutanol production. Furthermore, the strain overexpressing the isobutanol pathway on a self-replicating vector with the strong Ptrc promoter showed significantly higher gene expression and isobutanol production compared to the corresponding strains expressing the same operon introduced on the genome. Hence, this study demonstrates that Synechocystis endogenous AHDs have a high capacity for isobutanol production, and identifies kivd encoded α-ketoisovalerate decarboxylase as one of the likely bottlenecks for further isobutanol production.http://www.sciencedirect.com/science/article/pii/S2214030117300123CyanobacteriaAlcohol dehydrogenaseα-ketoisovalerate decarboxylaseSynechocystis PCC 6803Isobutanol production |
| spellingShingle | Rui Miao Xufeng Liu Elias Englund Pia Lindberg Peter Lindblad Isobutanol production in Synechocystis PCC 6803 using heterologous and endogenous alcohol dehydrogenases Metabolic Engineering Communications Cyanobacteria Alcohol dehydrogenase α-ketoisovalerate decarboxylase Synechocystis PCC 6803 Isobutanol production |
| title | Isobutanol production in Synechocystis PCC 6803 using heterologous and endogenous alcohol dehydrogenases |
| title_full | Isobutanol production in Synechocystis PCC 6803 using heterologous and endogenous alcohol dehydrogenases |
| title_fullStr | Isobutanol production in Synechocystis PCC 6803 using heterologous and endogenous alcohol dehydrogenases |
| title_full_unstemmed | Isobutanol production in Synechocystis PCC 6803 using heterologous and endogenous alcohol dehydrogenases |
| title_short | Isobutanol production in Synechocystis PCC 6803 using heterologous and endogenous alcohol dehydrogenases |
| title_sort | isobutanol production in synechocystis pcc 6803 using heterologous and endogenous alcohol dehydrogenases |
| topic | Cyanobacteria Alcohol dehydrogenase α-ketoisovalerate decarboxylase Synechocystis PCC 6803 Isobutanol production |
| url | http://www.sciencedirect.com/science/article/pii/S2214030117300123 |
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