Nonlinear optical microscopy for histology of fresh normal and cancerous pancreatic tissues.
BACKGROUND: Pancreatic cancer is a lethal disease with a 5-year survival rate of only 1-5%. The acceleration of intraoperative histological examination would be beneficial for better management of pancreatic cancer, suggesting an improved survival. Nonlinear optical methods based on two-photon excit...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2012-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3360059?pdf=render |
| _version_ | 1819229025483096064 |
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| author | Wenyan Hu Gang Zhao Chunyou Wang Jungang Zhang Ling Fu |
| author_facet | Wenyan Hu Gang Zhao Chunyou Wang Jungang Zhang Ling Fu |
| author_sort | Wenyan Hu |
| collection | DOAJ |
| description | BACKGROUND: Pancreatic cancer is a lethal disease with a 5-year survival rate of only 1-5%. The acceleration of intraoperative histological examination would be beneficial for better management of pancreatic cancer, suggesting an improved survival. Nonlinear optical methods based on two-photon excited fluorescence (TPEF) and second harmonic generation (SHG) of intrinsic optical biomarkers show the ability to visualize the morphology of fresh tissues associated with histology, which is promising for real-time intraoperative evaluation of pancreatic cancer. METHODOLOGY/PRINCIPAL FINDINGS: In order to investigate whether the nonlinear optical imaging methods have the ability to characterize pancreatic histology at cellular resolution, we studied different types of pancreatic tissues by using label-free TPEF and SHG. Compared with other routine methods for the preparation of specimens, fresh tissues without processing were found to be most suitable for nonlinear optical imaging of pancreatic tissues. The detailed morphology of the normal rat pancreas was observed and related with the standard histological images. Comparatively speaking, the preliminary images of a small number of chemical-induced pancreatic cancer tissues showed visible neoplastic differences in the morphology of cells and extracellular matrix. The subcutaneous pancreatic tumor xenografts were further observed using the nonlinear optical microscopy, showing that most cells are leucocytes at 5 days after implantation, the tumor cells begin to proliferate at 10 days after implantation, and the extracellular collagen fibers become disordered as the xenografts grow. CONCLUSIONS/SIGNIFICANCE: In this study, nonlinear optical imaging was used to characterize the morphological details of fresh pancreatic tissues for the first time. We demonstrate that it is possible to provide real-time histological evaluation of pancreatic cancer by the nonlinear optical methods, which present an opportunity for the characterization of the progress of spontaneous pancreatic cancer and further application in a non-invasive manner. |
| first_indexed | 2024-12-23T11:06:37Z |
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| id | doaj.art-01f9e748a4da480d902fa5e140d1443f |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-23T11:06:37Z |
| publishDate | 2012-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-01f9e748a4da480d902fa5e140d1443f2022-12-21T17:49:28ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-0175e3796210.1371/journal.pone.0037962Nonlinear optical microscopy for histology of fresh normal and cancerous pancreatic tissues.Wenyan HuGang ZhaoChunyou WangJungang ZhangLing FuBACKGROUND: Pancreatic cancer is a lethal disease with a 5-year survival rate of only 1-5%. The acceleration of intraoperative histological examination would be beneficial for better management of pancreatic cancer, suggesting an improved survival. Nonlinear optical methods based on two-photon excited fluorescence (TPEF) and second harmonic generation (SHG) of intrinsic optical biomarkers show the ability to visualize the morphology of fresh tissues associated with histology, which is promising for real-time intraoperative evaluation of pancreatic cancer. METHODOLOGY/PRINCIPAL FINDINGS: In order to investigate whether the nonlinear optical imaging methods have the ability to characterize pancreatic histology at cellular resolution, we studied different types of pancreatic tissues by using label-free TPEF and SHG. Compared with other routine methods for the preparation of specimens, fresh tissues without processing were found to be most suitable for nonlinear optical imaging of pancreatic tissues. The detailed morphology of the normal rat pancreas was observed and related with the standard histological images. Comparatively speaking, the preliminary images of a small number of chemical-induced pancreatic cancer tissues showed visible neoplastic differences in the morphology of cells and extracellular matrix. The subcutaneous pancreatic tumor xenografts were further observed using the nonlinear optical microscopy, showing that most cells are leucocytes at 5 days after implantation, the tumor cells begin to proliferate at 10 days after implantation, and the extracellular collagen fibers become disordered as the xenografts grow. CONCLUSIONS/SIGNIFICANCE: In this study, nonlinear optical imaging was used to characterize the morphological details of fresh pancreatic tissues for the first time. We demonstrate that it is possible to provide real-time histological evaluation of pancreatic cancer by the nonlinear optical methods, which present an opportunity for the characterization of the progress of spontaneous pancreatic cancer and further application in a non-invasive manner.http://europepmc.org/articles/PMC3360059?pdf=render |
| spellingShingle | Wenyan Hu Gang Zhao Chunyou Wang Jungang Zhang Ling Fu Nonlinear optical microscopy for histology of fresh normal and cancerous pancreatic tissues. PLoS ONE |
| title | Nonlinear optical microscopy for histology of fresh normal and cancerous pancreatic tissues. |
| title_full | Nonlinear optical microscopy for histology of fresh normal and cancerous pancreatic tissues. |
| title_fullStr | Nonlinear optical microscopy for histology of fresh normal and cancerous pancreatic tissues. |
| title_full_unstemmed | Nonlinear optical microscopy for histology of fresh normal and cancerous pancreatic tissues. |
| title_short | Nonlinear optical microscopy for histology of fresh normal and cancerous pancreatic tissues. |
| title_sort | nonlinear optical microscopy for histology of fresh normal and cancerous pancreatic tissues |
| url | http://europepmc.org/articles/PMC3360059?pdf=render |
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