The probiotic effects, dose, and duration of lactic acid bacteria on disease resistance in Litopenaeus vannamei
Four lactic acid bacteria with a high capacity for acid production were screened from 113 lactic acid bacteria (LAB) isolated from aquaculture systems, and were identified as Lactococcus lactis (S1 and S2), Enterococcus faecali (F3 and F7) by 16S rDNA sequencing. These four candidates exhibited favo...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-10-01
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| Series: | Aquaculture Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352513422002952 |
| _version_ | 1828733847772069888 |
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| author | Xiaoni Cai Jin-shun Wen Hao Long Wei Ren Xiang Zhang Ai-you Huang Zhen-yu Xie |
| author_facet | Xiaoni Cai Jin-shun Wen Hao Long Wei Ren Xiang Zhang Ai-you Huang Zhen-yu Xie |
| author_sort | Xiaoni Cai |
| collection | DOAJ |
| description | Four lactic acid bacteria with a high capacity for acid production were screened from 113 lactic acid bacteria (LAB) isolated from aquaculture systems, and were identified as Lactococcus lactis (S1 and S2), Enterococcus faecali (F3 and F7) by 16S rDNA sequencing. These four candidates exhibited favorable probiotic properties, including no haemolytic activity, good auto-aggregation performance, tolerance to simulated gastric fluid, and antagonistic activity against pathogens. To determine the ideal probiotic feeding dose and duration that would maximize protection against pathogens, L. vannamei were fed a probiotic supplemented diet at a series of concentrations for up to 60 days, and then challenged with Vibrio harveyi every 15 days. In vivo studies demonstrated that supplementation with L. lactis (S1 and S2) and E. faecalis (F3 and F7) significantly improved growth performance and feed utilization in the probiotic-treated groups compared to the control group. The findings suggested that administration of F7 and S2 strains at 106 CFU g−1 for 30–45 d was more effective at suppressing V. harveyi infection than any of the other feeding regimens. This study establishes a theoretical foundation for the practical use of probiotics. |
| first_indexed | 2024-04-12T22:37:49Z |
| format | Article |
| id | doaj.art-01f9eb30ac274386855dac7753b6eb5f |
| institution | Directory Open Access Journal |
| issn | 2352-5134 |
| language | English |
| last_indexed | 2024-04-12T22:37:49Z |
| publishDate | 2022-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Aquaculture Reports |
| spelling | doaj.art-01f9eb30ac274386855dac7753b6eb5f2022-12-22T03:13:48ZengElsevierAquaculture Reports2352-51342022-10-0126101299The probiotic effects, dose, and duration of lactic acid bacteria on disease resistance in Litopenaeus vannameiXiaoni Cai0Jin-shun Wen1Hao Long2Wei Ren3Xiang Zhang4Ai-you Huang5Zhen-yu Xie6Sanya Nanfan Research Institute of Hainan University, Hainan University, Sanya 572025, Hainan Province, PR China; State Key Laboratory of Marine Resource Utilization in the South China Sea, Hainan University, Haikou 570228, Hainan Province, PR China; Key Laboratory of Tropical Hydrobiology and Biotechnology of Hainan Province, Haikou 570228, Hainan Province, PR China; College of Marine Sciences, Hainan University, Haikou 570228, Hainan Province, PR ChinaState Key Laboratory of Marine Resource Utilization in the South China Sea, Hainan University, Haikou 570228, Hainan Province, PR China; Key Laboratory of Tropical Hydrobiology and Biotechnology of Hainan Province, Haikou 570228, Hainan Province, PR ChinaState Key Laboratory of Marine Resource Utilization in the South China Sea, Hainan University, Haikou 570228, Hainan Province, PR ChinaState Key Laboratory of Marine Resource Utilization in the South China Sea, Hainan University, Haikou 570228, Hainan Province, PR China; Key Laboratory of Tropical Hydrobiology and Biotechnology of Hainan Province, Haikou 570228, Hainan Province, PR China; College of Marine Sciences, Hainan University, Haikou 570228, Hainan Province, PR ChinaState Key Laboratory of Marine Resource Utilization in the South China Sea, Hainan University, Haikou 570228, Hainan Province, PR China; Key Laboratory of Tropical Hydrobiology and Biotechnology of Hainan Province, Haikou 570228, Hainan Province, PR China; College of Marine Sciences, Hainan University, Haikou 570228, Hainan Province, PR ChinaState Key Laboratory of Marine Resource Utilization in the South China Sea, Hainan University, Haikou 570228, Hainan Province, PR China; Key Laboratory of Tropical Hydrobiology and Biotechnology of Hainan Province, Haikou 570228, Hainan Province, PR China; College of Marine Sciences, Hainan University, Haikou 570228, Hainan Province, PR ChinaState Key Laboratory of Marine Resource Utilization in the South China Sea, Hainan University, Haikou 570228, Hainan Province, PR China; Key Laboratory of Tropical Hydrobiology and Biotechnology of Hainan Province, Haikou 570228, Hainan Province, PR China; College of Marine Sciences, Hainan University, Haikou 570228, Hainan Province, PR China; Corresponding author at: State Key Laboratory of Marine Resource Utilization in the South China Sea, Hainan University, Haikou 570228, Hainan Province, PR China.Four lactic acid bacteria with a high capacity for acid production were screened from 113 lactic acid bacteria (LAB) isolated from aquaculture systems, and were identified as Lactococcus lactis (S1 and S2), Enterococcus faecali (F3 and F7) by 16S rDNA sequencing. These four candidates exhibited favorable probiotic properties, including no haemolytic activity, good auto-aggregation performance, tolerance to simulated gastric fluid, and antagonistic activity against pathogens. To determine the ideal probiotic feeding dose and duration that would maximize protection against pathogens, L. vannamei were fed a probiotic supplemented diet at a series of concentrations for up to 60 days, and then challenged with Vibrio harveyi every 15 days. In vivo studies demonstrated that supplementation with L. lactis (S1 and S2) and E. faecalis (F3 and F7) significantly improved growth performance and feed utilization in the probiotic-treated groups compared to the control group. The findings suggested that administration of F7 and S2 strains at 106 CFU g−1 for 30–45 d was more effective at suppressing V. harveyi infection than any of the other feeding regimens. This study establishes a theoretical foundation for the practical use of probiotics.http://www.sciencedirect.com/science/article/pii/S2352513422002952ProbioticsFeeding durationFeeding dosageLitopenaeus vannameiMortality rate |
| spellingShingle | Xiaoni Cai Jin-shun Wen Hao Long Wei Ren Xiang Zhang Ai-you Huang Zhen-yu Xie The probiotic effects, dose, and duration of lactic acid bacteria on disease resistance in Litopenaeus vannamei Aquaculture Reports Probiotics Feeding duration Feeding dosage Litopenaeus vannamei Mortality rate |
| title | The probiotic effects, dose, and duration of lactic acid bacteria on disease resistance in Litopenaeus vannamei |
| title_full | The probiotic effects, dose, and duration of lactic acid bacteria on disease resistance in Litopenaeus vannamei |
| title_fullStr | The probiotic effects, dose, and duration of lactic acid bacteria on disease resistance in Litopenaeus vannamei |
| title_full_unstemmed | The probiotic effects, dose, and duration of lactic acid bacteria on disease resistance in Litopenaeus vannamei |
| title_short | The probiotic effects, dose, and duration of lactic acid bacteria on disease resistance in Litopenaeus vannamei |
| title_sort | probiotic effects dose and duration of lactic acid bacteria on disease resistance in litopenaeus vannamei |
| topic | Probiotics Feeding duration Feeding dosage Litopenaeus vannamei Mortality rate |
| url | http://www.sciencedirect.com/science/article/pii/S2352513422002952 |
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