Recent Advances in Chemical Biology Using Benzophenones and Diazirines as Radical Precursors
The use of light-activated chemical probes to study biological interactions was first discovered in the 1960s, and has since found many applications in studying diseases and gaining deeper insight into various cellular mechanisms involving protein–protein, protein–nucleic acid, protein–ligand (drug,...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-05-01
|
| Series: | Molecules |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1420-3049/25/10/2285 |
| _version_ | 1797568137457041408 |
|---|---|
| author | Muhammad Murtaza Hassan Olasunkanmi O. Olaoye |
| author_facet | Muhammad Murtaza Hassan Olasunkanmi O. Olaoye |
| author_sort | Muhammad Murtaza Hassan |
| collection | DOAJ |
| description | The use of light-activated chemical probes to study biological interactions was first discovered in the 1960s, and has since found many applications in studying diseases and gaining deeper insight into various cellular mechanisms involving protein–protein, protein–nucleic acid, protein–ligand (drug, probe), and protein–co-factor interactions, among others. This technique, often referred to as photoaffinity labelling, uses radical precursors that react almost instantaneously to yield spatial and temporal information about the nature of the interaction and the interacting partner(s). This review focuses on the recent advances in chemical biology in the use of benzophenones and diazirines, two of the most commonly known light-activatable radical precursors, with a focus on the last three years, and is intended to provide a solid understanding of their chemical and biological principles and their applications. |
| first_indexed | 2024-03-10T19:52:06Z |
| format | Article |
| id | doaj.art-01fb0a8716a645f5a596bcada297932d |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T19:52:06Z |
| publishDate | 2020-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-01fb0a8716a645f5a596bcada297932d2023-11-20T00:16:47ZengMDPI AGMolecules1420-30492020-05-012510228510.3390/molecules25102285Recent Advances in Chemical Biology Using Benzophenones and Diazirines as Radical PrecursorsMuhammad Murtaza Hassan0Olasunkanmi O. Olaoye1Department of Chemical and Physical Sciences, University of Toronto Mississauga, 3359 Mississauga Road North, Mississauga, ON L5L 1C6, CanadaDepartment of Chemical and Physical Sciences, University of Toronto Mississauga, 3359 Mississauga Road North, Mississauga, ON L5L 1C6, CanadaThe use of light-activated chemical probes to study biological interactions was first discovered in the 1960s, and has since found many applications in studying diseases and gaining deeper insight into various cellular mechanisms involving protein–protein, protein–nucleic acid, protein–ligand (drug, probe), and protein–co-factor interactions, among others. This technique, often referred to as photoaffinity labelling, uses radical precursors that react almost instantaneously to yield spatial and temporal information about the nature of the interaction and the interacting partner(s). This review focuses on the recent advances in chemical biology in the use of benzophenones and diazirines, two of the most commonly known light-activatable radical precursors, with a focus on the last three years, and is intended to provide a solid understanding of their chemical and biological principles and their applications.https://www.mdpi.com/1420-3049/25/10/2285photoaffinity labellingbenzophenonediazirineradical precursorsinteractomeSABRE |
| spellingShingle | Muhammad Murtaza Hassan Olasunkanmi O. Olaoye Recent Advances in Chemical Biology Using Benzophenones and Diazirines as Radical Precursors Molecules photoaffinity labelling benzophenone diazirine radical precursors interactome SABRE |
| title | Recent Advances in Chemical Biology Using Benzophenones and Diazirines as Radical Precursors |
| title_full | Recent Advances in Chemical Biology Using Benzophenones and Diazirines as Radical Precursors |
| title_fullStr | Recent Advances in Chemical Biology Using Benzophenones and Diazirines as Radical Precursors |
| title_full_unstemmed | Recent Advances in Chemical Biology Using Benzophenones and Diazirines as Radical Precursors |
| title_short | Recent Advances in Chemical Biology Using Benzophenones and Diazirines as Radical Precursors |
| title_sort | recent advances in chemical biology using benzophenones and diazirines as radical precursors |
| topic | photoaffinity labelling benzophenone diazirine radical precursors interactome SABRE |
| url | https://www.mdpi.com/1420-3049/25/10/2285 |
| work_keys_str_mv | AT muhammadmurtazahassan recentadvancesinchemicalbiologyusingbenzophenonesanddiazirinesasradicalprecursors AT olasunkanmioolaoye recentadvancesinchemicalbiologyusingbenzophenonesanddiazirinesasradicalprecursors |