Molecular mechanisms of low-temperature sensitivity in tropical/subtropical plants: a case study of Casuarina equisetifolia
Low temperature is a limiting factor affecting plant growth and development. Casuarina equisetifolia, a typical tropical and subtropical tree important for the ecological restoration of coastal beaches, is sensitive to cold stress. By comparing cold tolerance between C. equisetifolia and Arabidopsis...
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| Format: | Article |
| Language: | English |
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Maximum Academic Press
2023-01-01
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| Series: | Forestry Research |
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| Online Access: | https://www.maxapress.com/article/doi/10.48130/FR-2023-0020 |
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| author | Huimin Ren Yue Zhong Liangyu Guo Jamshaid Hussian Chen Zhou Youzhi Cao Wenwu Wu Shenkui Liu Guoning Qi |
| author_facet | Huimin Ren Yue Zhong Liangyu Guo Jamshaid Hussian Chen Zhou Youzhi Cao Wenwu Wu Shenkui Liu Guoning Qi |
| author_sort | Huimin Ren |
| collection | DOAJ |
| description | Low temperature is a limiting factor affecting plant growth and development. Casuarina equisetifolia, a typical tropical and subtropical tree important for the ecological restoration of coastal beaches, is sensitive to cold stress. By comparing cold tolerance between C. equisetifolia and Arabidopsis, we investigated the molecular basis underlying the cold sensitivity of C. equisetifolia. Transcriptomic analysis showed that the number of cold-induced genes in C. equisetifolia was significantly less than that in Arabidopsis, and notably, the response of cold-induced genes was also delayed in C. equisetifolia. Among the cold-induced genes, C-repeat binding factors (CBFs), the major transcription factors in cold acclimation in Arabidopsis, showed a delayed cold-induced expression in C. equisetifolia, despite that C. equisetifolia CBFs could restore the low temperature-sensitive phenotype of Arabidopsis cbfs triple mutants. Interestingly, some key cold-responsive genes (e.g., COR15A and COR15B) targeted by Arabidopsis CBF were absent in the C. equisetifolia genome and many cold-responsive genes in C. equisetifolia lacked the DRE element (i.e., CBF binding cis-element). Moreover, like in C. equisetifolia, many COR genes in other tropical/subtropical plants lacked the DRE element or were directly missing. These two factors could be the underlying reasons for the low-temperature sensitivity of C. equisetifolia and other tropical/subtropical plants. |
| first_indexed | 2024-03-07T20:08:32Z |
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| institution | Directory Open Access Journal |
| issn | 2767-3812 |
| language | English |
| last_indexed | 2024-03-07T20:08:32Z |
| publishDate | 2023-01-01 |
| publisher | Maximum Academic Press |
| record_format | Article |
| series | Forestry Research |
| spelling | doaj.art-01fc283862f845e1ad575516771c0a8b2024-02-28T01:55:38ZengMaximum Academic PressForestry Research2767-38122023-01-013111910.48130/FR-2023-0020FR-2023-0020Molecular mechanisms of low-temperature sensitivity in tropical/subtropical plants: a case study of Casuarina equisetifoliaHuimin Ren0Yue Zhong1Liangyu Guo2Jamshaid Hussian3Chen Zhou4Youzhi Cao5Wenwu Wu6Shenkui Liu7Guoning Qi8State Key Laboratory of Subtropical Silviculture, School of Forestry and Biotechnology, Zhejiang A & F University, Hangzhou 311300, Zhejiang, ChinaState Key Laboratory of Subtropical Silviculture, School of Forestry and Biotechnology, Zhejiang A & F University, Hangzhou 311300, Zhejiang, ChinaState Key Laboratory of Subtropical Silviculture, School of Forestry and Biotechnology, Zhejiang A & F University, Hangzhou 311300, Zhejiang, ChinaDepartment of Biotechnology, COMSATS University Islamabad, Abbottabad Campus, 22060, University Road Abbottabad, PakistanState Key Laboratory of Subtropical Silviculture, School of Forestry and Biotechnology, Zhejiang A & F University, Hangzhou 311300, Zhejiang, ChinaInstitute of Functional Nano & Soft Materials (FUNSOM), Soochow University, Suzhou 215123, Jiangsu, ChinaState Key Laboratory of Subtropical Silviculture, School of Forestry and Biotechnology, Zhejiang A & F University, Hangzhou 311300, Zhejiang, ChinaState Key Laboratory of Subtropical Silviculture, School of Forestry and Biotechnology, Zhejiang A & F University, Hangzhou 311300, Zhejiang, ChinaState Key Laboratory of Subtropical Silviculture, School of Forestry and Biotechnology, Zhejiang A & F University, Hangzhou 311300, Zhejiang, ChinaLow temperature is a limiting factor affecting plant growth and development. Casuarina equisetifolia, a typical tropical and subtropical tree important for the ecological restoration of coastal beaches, is sensitive to cold stress. By comparing cold tolerance between C. equisetifolia and Arabidopsis, we investigated the molecular basis underlying the cold sensitivity of C. equisetifolia. Transcriptomic analysis showed that the number of cold-induced genes in C. equisetifolia was significantly less than that in Arabidopsis, and notably, the response of cold-induced genes was also delayed in C. equisetifolia. Among the cold-induced genes, C-repeat binding factors (CBFs), the major transcription factors in cold acclimation in Arabidopsis, showed a delayed cold-induced expression in C. equisetifolia, despite that C. equisetifolia CBFs could restore the low temperature-sensitive phenotype of Arabidopsis cbfs triple mutants. Interestingly, some key cold-responsive genes (e.g., COR15A and COR15B) targeted by Arabidopsis CBF were absent in the C. equisetifolia genome and many cold-responsive genes in C. equisetifolia lacked the DRE element (i.e., CBF binding cis-element). Moreover, like in C. equisetifolia, many COR genes in other tropical/subtropical plants lacked the DRE element or were directly missing. These two factors could be the underlying reasons for the low-temperature sensitivity of C. equisetifolia and other tropical/subtropical plants.https://www.maxapress.com/article/doi/10.48130/FR-2023-0020cold stresscold acclimationtranscriptomecbfcold responsive genecasuarina equisetifolia |
| spellingShingle | Huimin Ren Yue Zhong Liangyu Guo Jamshaid Hussian Chen Zhou Youzhi Cao Wenwu Wu Shenkui Liu Guoning Qi Molecular mechanisms of low-temperature sensitivity in tropical/subtropical plants: a case study of Casuarina equisetifolia Forestry Research cold stress cold acclimation transcriptome cbf cold responsive gene casuarina equisetifolia |
| title | Molecular mechanisms of low-temperature sensitivity in tropical/subtropical plants: a case study of Casuarina equisetifolia |
| title_full | Molecular mechanisms of low-temperature sensitivity in tropical/subtropical plants: a case study of Casuarina equisetifolia |
| title_fullStr | Molecular mechanisms of low-temperature sensitivity in tropical/subtropical plants: a case study of Casuarina equisetifolia |
| title_full_unstemmed | Molecular mechanisms of low-temperature sensitivity in tropical/subtropical plants: a case study of Casuarina equisetifolia |
| title_short | Molecular mechanisms of low-temperature sensitivity in tropical/subtropical plants: a case study of Casuarina equisetifolia |
| title_sort | molecular mechanisms of low temperature sensitivity in tropical subtropical plants a case study of casuarina equisetifolia |
| topic | cold stress cold acclimation transcriptome cbf cold responsive gene casuarina equisetifolia |
| url | https://www.maxapress.com/article/doi/10.48130/FR-2023-0020 |
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