In-situ recording of ionic currents in projection neurons and Kenyon cells in the olfactory pathway of the honeybee.

The honeybee olfactory pathway comprises an intriguing pattern of convergence and divergence: ~60.000 olfactory sensory neurons (OSN) convey olfactory information on ~900 projection neurons (PN) in the antennal lobe (AL). To transmit this information reliably, PNs employ relatively high spiking freq...

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Main Authors: Jan Kropf, Wolfgang Rössler
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2018-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC5774781?pdf=render
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author Jan Kropf
Wolfgang Rössler
author_facet Jan Kropf
Wolfgang Rössler
author_sort Jan Kropf
collection DOAJ
description The honeybee olfactory pathway comprises an intriguing pattern of convergence and divergence: ~60.000 olfactory sensory neurons (OSN) convey olfactory information on ~900 projection neurons (PN) in the antennal lobe (AL). To transmit this information reliably, PNs employ relatively high spiking frequencies with complex patterns. PNs project via a dual olfactory pathway to the mushroom bodies (MB). This pathway comprises the medial (m-ALT) and the lateral antennal lobe tract (l-ALT). PNs from both tracts transmit information from a wide range of similar odors, but with distinct differences in coding properties. In the MBs, PNs form synapses with many Kenyon cells (KC) that encode odors in a spatially and temporally sparse way. The transformation from complex information coding to sparse coding is a well-known phenomenon in insect olfactory coding. Intrinsic neuronal properties as well as GABAergic inhibition are thought to contribute to this change in odor representation. In the present study, we identified intrinsic neuronal properties promoting coding differences between PNs and KCs using in-situ patch-clamp recordings in the intact brain. We found very prominent K+ currents in KCs clearly differing from the PN currents. This suggests that odor coding differences between PNs and KCs may be caused by differences in their specific ion channel properties. Comparison of ionic currents of m- and l-ALT PNs did not reveal any differences at a qualitative level.
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spelling doaj.art-01fc9232623d4232b4a429dff3a695a72022-12-21T23:23:00ZengPublic Library of Science (PLoS)PLoS ONE1932-62032018-01-01131e019142510.1371/journal.pone.0191425In-situ recording of ionic currents in projection neurons and Kenyon cells in the olfactory pathway of the honeybee.Jan KropfWolfgang RösslerThe honeybee olfactory pathway comprises an intriguing pattern of convergence and divergence: ~60.000 olfactory sensory neurons (OSN) convey olfactory information on ~900 projection neurons (PN) in the antennal lobe (AL). To transmit this information reliably, PNs employ relatively high spiking frequencies with complex patterns. PNs project via a dual olfactory pathway to the mushroom bodies (MB). This pathway comprises the medial (m-ALT) and the lateral antennal lobe tract (l-ALT). PNs from both tracts transmit information from a wide range of similar odors, but with distinct differences in coding properties. In the MBs, PNs form synapses with many Kenyon cells (KC) that encode odors in a spatially and temporally sparse way. The transformation from complex information coding to sparse coding is a well-known phenomenon in insect olfactory coding. Intrinsic neuronal properties as well as GABAergic inhibition are thought to contribute to this change in odor representation. In the present study, we identified intrinsic neuronal properties promoting coding differences between PNs and KCs using in-situ patch-clamp recordings in the intact brain. We found very prominent K+ currents in KCs clearly differing from the PN currents. This suggests that odor coding differences between PNs and KCs may be caused by differences in their specific ion channel properties. Comparison of ionic currents of m- and l-ALT PNs did not reveal any differences at a qualitative level.http://europepmc.org/articles/PMC5774781?pdf=render
spellingShingle Jan Kropf
Wolfgang Rössler
In-situ recording of ionic currents in projection neurons and Kenyon cells in the olfactory pathway of the honeybee.
PLoS ONE
title In-situ recording of ionic currents in projection neurons and Kenyon cells in the olfactory pathway of the honeybee.
title_full In-situ recording of ionic currents in projection neurons and Kenyon cells in the olfactory pathway of the honeybee.
title_fullStr In-situ recording of ionic currents in projection neurons and Kenyon cells in the olfactory pathway of the honeybee.
title_full_unstemmed In-situ recording of ionic currents in projection neurons and Kenyon cells in the olfactory pathway of the honeybee.
title_short In-situ recording of ionic currents in projection neurons and Kenyon cells in the olfactory pathway of the honeybee.
title_sort in situ recording of ionic currents in projection neurons and kenyon cells in the olfactory pathway of the honeybee
url http://europepmc.org/articles/PMC5774781?pdf=render
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