Drought-Induced Regulatory Cascades and Their Effects on the Nutritional Quality of Developing Potato Tubers
Competition for scarce water resources and the continued effects of global warming exacerbate current constraints on potato crop production. While plants’ response to drought in above-ground tissues has been well documented, the regulatory cascades and subsequent nutritive changes in developing tube...
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MDPI AG
2020-07-01
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| Series: | Genes |
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| Online Access: | https://www.mdpi.com/2073-4425/11/8/864 |
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| author | Letitia Da Ros Raed Elferjani Raju Soolanayakanahally Sateesh Kagale Shankar Pahari Manoj Kulkarni Jazeem Wahab Benoit Bizimungu |
| author_facet | Letitia Da Ros Raed Elferjani Raju Soolanayakanahally Sateesh Kagale Shankar Pahari Manoj Kulkarni Jazeem Wahab Benoit Bizimungu |
| author_sort | Letitia Da Ros |
| collection | DOAJ |
| description | Competition for scarce water resources and the continued effects of global warming exacerbate current constraints on potato crop production. While plants’ response to drought in above-ground tissues has been well documented, the regulatory cascades and subsequent nutritive changes in developing tubers have been largely unexplored. Using the commercial Canadian cultivar “Vigor”, plants were subjected to a gradual drought treatment under high tunnels causing a 4 °C increase in the canopy temperature. Tubers were sampled for RNAseq and metabolite analysis. Approximately 2600 genes and 3898 transcripts were differentially expressed by at least 4-fold in drought-stressed potato tubers, with 75% and 69% being down-regulated, respectively. A further 229 small RNAs were implicated in gene regulation during drought. Expression of several small RNA clusters negatively correlated with expression of their six target patatin genes, suggesting involvement in the regulation of storage proteins during drought. The comparison of protein homologues between <i>Solanum tuberosum</i> L. and <i>Arabidopsis thaliana</i> L. indicated that down-regulated genes were associated with phenylpropanoid and carotenoid biosynthesis. As is indicative of reduced flow through the phenylpropanoid pathway, phenylalanine accumulated in drought-stressed tubers. This suggests that there may be nutritive implications to drought stress occurring during the potato tuber bulking phase in sensitive cultivars. |
| first_indexed | 2024-03-10T18:06:44Z |
| format | Article |
| id | doaj.art-01ff952d7af941788476d5ebe3abf5d5 |
| institution | Directory Open Access Journal |
| issn | 2073-4425 |
| language | English |
| last_indexed | 2024-03-10T18:06:44Z |
| publishDate | 2020-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Genes |
| spelling | doaj.art-01ff952d7af941788476d5ebe3abf5d52023-11-20T08:25:52ZengMDPI AGGenes2073-44252020-07-0111886410.3390/genes11080864Drought-Induced Regulatory Cascades and Their Effects on the Nutritional Quality of Developing Potato TubersLetitia Da Ros0Raed Elferjani1Raju Soolanayakanahally2Sateesh Kagale3Shankar Pahari4Manoj Kulkarni5Jazeem Wahab6Benoit Bizimungu7Faculty of Forestry, University of British Columbia, Vancouver, BC V6T1Z4, CanadaSaskatoon Research and Development Centre, Agriculture and Agri-Food Canada, Saskatoon, SK S7N0X2, CanadaSaskatoon Research and Development Centre, Agriculture and Agri-Food Canada, Saskatoon, SK S7N0X2, CanadaNational Research Council Canada, Saskatoon, SK S7N0W9, CanadaSaskatoon Research and Development Centre, Agriculture and Agri-Food Canada, Saskatoon, SK S7N0X2, CanadaNational Research Council Canada, Saskatoon, SK S7N0W9, CanadaSaskatoon Research and Development Centre, Agriculture and Agri-Food Canada, Saskatoon, SK S7N0X2, CanadaFredericton Research and Development Centre, Agriculture and Agri-Food Canada, Fredericton, NB E3B4Z7, CanadaCompetition for scarce water resources and the continued effects of global warming exacerbate current constraints on potato crop production. While plants’ response to drought in above-ground tissues has been well documented, the regulatory cascades and subsequent nutritive changes in developing tubers have been largely unexplored. Using the commercial Canadian cultivar “Vigor”, plants were subjected to a gradual drought treatment under high tunnels causing a 4 °C increase in the canopy temperature. Tubers were sampled for RNAseq and metabolite analysis. Approximately 2600 genes and 3898 transcripts were differentially expressed by at least 4-fold in drought-stressed potato tubers, with 75% and 69% being down-regulated, respectively. A further 229 small RNAs were implicated in gene regulation during drought. Expression of several small RNA clusters negatively correlated with expression of their six target patatin genes, suggesting involvement in the regulation of storage proteins during drought. The comparison of protein homologues between <i>Solanum tuberosum</i> L. and <i>Arabidopsis thaliana</i> L. indicated that down-regulated genes were associated with phenylpropanoid and carotenoid biosynthesis. As is indicative of reduced flow through the phenylpropanoid pathway, phenylalanine accumulated in drought-stressed tubers. This suggests that there may be nutritive implications to drought stress occurring during the potato tuber bulking phase in sensitive cultivars.https://www.mdpi.com/2073-4425/11/8/864crop genetics<i>Solanum tuberosum</i>abiotic stressphenylpropanoidsessential amino acidtranscriptome |
| spellingShingle | Letitia Da Ros Raed Elferjani Raju Soolanayakanahally Sateesh Kagale Shankar Pahari Manoj Kulkarni Jazeem Wahab Benoit Bizimungu Drought-Induced Regulatory Cascades and Their Effects on the Nutritional Quality of Developing Potato Tubers Genes crop genetics <i>Solanum tuberosum</i> abiotic stress phenylpropanoids essential amino acid transcriptome |
| title | Drought-Induced Regulatory Cascades and Their Effects on the Nutritional Quality of Developing Potato Tubers |
| title_full | Drought-Induced Regulatory Cascades and Their Effects on the Nutritional Quality of Developing Potato Tubers |
| title_fullStr | Drought-Induced Regulatory Cascades and Their Effects on the Nutritional Quality of Developing Potato Tubers |
| title_full_unstemmed | Drought-Induced Regulatory Cascades and Their Effects on the Nutritional Quality of Developing Potato Tubers |
| title_short | Drought-Induced Regulatory Cascades and Their Effects on the Nutritional Quality of Developing Potato Tubers |
| title_sort | drought induced regulatory cascades and their effects on the nutritional quality of developing potato tubers |
| topic | crop genetics <i>Solanum tuberosum</i> abiotic stress phenylpropanoids essential amino acid transcriptome |
| url | https://www.mdpi.com/2073-4425/11/8/864 |
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