Identification of Toxigenic Aspergillus Species from Rice of Khuzestan and Mycotoxins in Imported Cereals
Background: Due to their high amount of carbohydrate and enough moisture, cereals are a good environment for the growth of toxigenic fungi. Because of the carcinogenicity and mutagenicity of mycotoxins, preventing them from entering the food chain is essential. Therefore, the present study was condu...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Farname
2019-12-01
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| Series: | Iranian Journal of Medical Microbiology |
| Subjects: | |
| Online Access: | http://ijmm.ir/article-1-971-en.html |
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| author | Razie Ranjbar Mohammad Roayaei Ardakani Mehdi Mehrabi Kushki Iraj kazeminezhad |
| author_facet | Razie Ranjbar Mohammad Roayaei Ardakani Mehdi Mehrabi Kushki Iraj kazeminezhad |
| author_sort | Razie Ranjbar |
| collection | DOAJ |
| description | Background: Due to their high amount of carbohydrate and enough moisture, cereals are a good environment for the growth of toxigenic fungi. Because of the carcinogenicity and mutagenicity of mycotoxins, preventing them from entering the food chain is essential. Therefore, the present study was conducted to determine the amount and type of contaminated imported cereals and rice produced in Khuzestan province
Materials & Methods: In October and November 2015, a total of 50 random samples of rice was collected from paddy fields. Aspergillus were identified based on available diagnostic criteria and PCR. The amount and type of aflatoxin in rice samples and mycotoxins in imported cereals (winter 2015 to autumn 2016) were evaluated by HPLC
Result: Based on one sample t-test and comparing the mean of mycotoxins contaminating cereals in different seasons with national maximum standard, the amount of mycotoxins in barley and wheat were within the standard range but %8.4 of corn was higher than the permitted level (ppb5). Analysis of aflatoxins in rice also showed that 16 samples were contaminated with aflatoxin B1. Aspergillus flavus was the major pollutant (%42.1) isolated from rice.
Conclusion: Aspergillus flavus is the major producer of aflatoxin B1 in domestic rice. Examination of imported cereals also showed high rates of fungal growth and production of secondary metabolites, possibly due to inadequate storage conditions, high temperature and humidity. Therefore, it is recommended to strengthen the monitoring tools in the processing and storage of rice and cereals. |
| first_indexed | 2024-04-11T18:41:29Z |
| format | Article |
| id | doaj.art-020a9a46c7d24d159ce4577793be2f78 |
| institution | Directory Open Access Journal |
| issn | 1735-8612 2345-4342 |
| language | English |
| last_indexed | 2024-04-11T18:41:29Z |
| publishDate | 2019-12-01 |
| publisher | Farname |
| record_format | Article |
| series | Iranian Journal of Medical Microbiology |
| spelling | doaj.art-020a9a46c7d24d159ce4577793be2f782022-12-22T04:08:57ZengFarnameIranian Journal of Medical Microbiology1735-86122345-43422019-12-01135355373Identification of Toxigenic Aspergillus Species from Rice of Khuzestan and Mycotoxins in Imported CerealsRazie Ranjbar0Mohammad Roayaei Ardakani1Mehdi Mehrabi Kushki2Iraj kazeminezhad3 Department of Biology, Faculty of Sciences, Shahid Chamran University of Ahvaz, Ahvaz, Iran Department of Biology, Faculty of Sciences, Shahid Chamran University of Ahvaz, Ahvaz, Iran Department of Plant Protection, Faculty of Agriculture, Shahid Chamran University of Ahvaz, Ahvaz, Iran Department of Physic, Faculty of Sciences, Shahid Chamran University of Ahvaz, Ahvaz, Iran Background: Due to their high amount of carbohydrate and enough moisture, cereals are a good environment for the growth of toxigenic fungi. Because of the carcinogenicity and mutagenicity of mycotoxins, preventing them from entering the food chain is essential. Therefore, the present study was conducted to determine the amount and type of contaminated imported cereals and rice produced in Khuzestan province Materials & Methods: In October and November 2015, a total of 50 random samples of rice was collected from paddy fields. Aspergillus were identified based on available diagnostic criteria and PCR. The amount and type of aflatoxin in rice samples and mycotoxins in imported cereals (winter 2015 to autumn 2016) were evaluated by HPLC Result: Based on one sample t-test and comparing the mean of mycotoxins contaminating cereals in different seasons with national maximum standard, the amount of mycotoxins in barley and wheat were within the standard range but %8.4 of corn was higher than the permitted level (ppb5). Analysis of aflatoxins in rice also showed that 16 samples were contaminated with aflatoxin B1. Aspergillus flavus was the major pollutant (%42.1) isolated from rice. Conclusion: Aspergillus flavus is the major producer of aflatoxin B1 in domestic rice. Examination of imported cereals also showed high rates of fungal growth and production of secondary metabolites, possibly due to inadequate storage conditions, high temperature and humidity. Therefore, it is recommended to strengthen the monitoring tools in the processing and storage of rice and cereals.http://ijmm.ir/article-1-971-en.htmlmycotoxinhplccarcinogenicaspergilluscereals |
| spellingShingle | Razie Ranjbar Mohammad Roayaei Ardakani Mehdi Mehrabi Kushki Iraj kazeminezhad Identification of Toxigenic Aspergillus Species from Rice of Khuzestan and Mycotoxins in Imported Cereals Iranian Journal of Medical Microbiology mycotoxin hplc carcinogenic aspergillus cereals |
| title | Identification of Toxigenic Aspergillus Species from Rice of Khuzestan and Mycotoxins in Imported Cereals |
| title_full | Identification of Toxigenic Aspergillus Species from Rice of Khuzestan and Mycotoxins in Imported Cereals |
| title_fullStr | Identification of Toxigenic Aspergillus Species from Rice of Khuzestan and Mycotoxins in Imported Cereals |
| title_full_unstemmed | Identification of Toxigenic Aspergillus Species from Rice of Khuzestan and Mycotoxins in Imported Cereals |
| title_short | Identification of Toxigenic Aspergillus Species from Rice of Khuzestan and Mycotoxins in Imported Cereals |
| title_sort | identification of toxigenic aspergillus species from rice of khuzestan and mycotoxins in imported cereals |
| topic | mycotoxin hplc carcinogenic aspergillus cereals |
| url | http://ijmm.ir/article-1-971-en.html |
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