Anserine Reverses Exercise-Induced Oxidative Stress and Preserves Cellular Homeostasis in Healthy Men
The study tested whether anserine (beta-alanyl-3-methyl-<span style="font-variant: small-caps;">l</span>-histidine), the active ingredient of chicken essence affects exercise-induced oxidative stress, cell integrity, and haematology biomarkers. In a randomized placebo-controlle...
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MDPI AG
2020-04-01
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| Series: | Nutrients |
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| Online Access: | https://www.mdpi.com/2072-6643/12/4/1146 |
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| author | Ahmad Alkhatib Wen-Hsin Feng Yi-Jen Huang Chia-Hua Kuo Chien-Wen Hou |
| author_facet | Ahmad Alkhatib Wen-Hsin Feng Yi-Jen Huang Chia-Hua Kuo Chien-Wen Hou |
| author_sort | Ahmad Alkhatib |
| collection | DOAJ |
| description | The study tested whether anserine (beta-alanyl-3-methyl-<span style="font-variant: small-caps;">l</span>-histidine), the active ingredient of chicken essence affects exercise-induced oxidative stress, cell integrity, and haematology biomarkers. In a randomized placebo-controlled repeated-measures design, ten healthy men ingested anserine in either a low dose (ANS-LD) 15 mg·kg<sup>−1</sup>·bw<sup>−1</sup>, high dose (ANS-HD) 30 mg·kg<sup>−1</sup>·bw<sup>−1</sup>, or placebo (PLA), following an exercise challenge (time to exhaustion), on three separate occasions. Anserine supplementation increased superoxide dismutase (SOD) by 50% (<i>p</i> < 0.001, effect size d = 0.8 for both ANS-LD and ANS-HD), and preserved catalase (CAT) activity suggesting an improved antioxidant activity. However, both ANS-LD and ANS-HD elevated glutathione disulfide (GSSG), (both <i>p</i> < 0.001, main treatment effect), and consequently lowered the glutathione to glutathione disulfide (GSH/GSSG) ratio compared with PLA (<i>p</i> < 0.01, main treatment effect), without significant effects on thiobarbituric acid active reactive substances (TBARS). Exercise-induced cell damage biomarkers of glutamic-oxaloacetic transaminase (GOT) and myoglobin were unaffected by anserine. There were slight but significant elevations in glutamate pyruvate transaminase (GPT) and creatine kinase isoenzyme (CKMB), especially in ANS-HD (<i>p</i> < 0.05) compared with ANS-LD or PLA. Haematological biomarkers were largely unaffected by anserine, its dose, and without interaction with post exercise time-course. However, compared with ANS-LD and PLA, ANS-HD increased the mean cell volume (MCV), and decreased the mean corpuscular haemoglobin concentration (MCHC) (<i>p</i> < 0.001). Anserine preserves cellular homoeostasis through enhanced antioxidant activity and protects cell integrity in healthy men, which is important for chronic disease prevention. However, anserine temporal elevated exercise-induced cell-damage, together with enhanced antioxidant activity and haematological responses suggest an augmented exercise-induced adaptative response and recovery. |
| first_indexed | 2024-03-10T20:20:52Z |
| format | Article |
| id | doaj.art-02102957ec064e28926c39a7ff8a58aa |
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| last_indexed | 2024-03-10T20:20:52Z |
| publishDate | 2020-04-01 |
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| spelling | doaj.art-02102957ec064e28926c39a7ff8a58aa2023-11-19T22:07:34ZengMDPI AGNutrients2072-66432020-04-01124114610.3390/nu12041146Anserine Reverses Exercise-Induced Oxidative Stress and Preserves Cellular Homeostasis in Healthy MenAhmad Alkhatib0Wen-Hsin Feng1Yi-Jen Huang2Chia-Hua Kuo3Chien-Wen Hou4Laboratory of Exercise Biochemistry, University of Taipei, No.101, Sec. 2, Zhongcheng Road., Shilin District, Taipei City 11153, TaiwanLaboratory of Exercise Biochemistry, University of Taipei, No.101, Sec. 2, Zhongcheng Road., Shilin District, Taipei City 11153, TaiwanOffice of Physical Education, Soochow University, No. 70, Linhsi Road, Shihlin District, Taipei City 11111, TaiwanLaboratory of Exercise Biochemistry, University of Taipei, No.101, Sec. 2, Zhongcheng Road., Shilin District, Taipei City 11153, TaiwanLaboratory of Exercise Biochemistry, University of Taipei, No.101, Sec. 2, Zhongcheng Road., Shilin District, Taipei City 11153, TaiwanThe study tested whether anserine (beta-alanyl-3-methyl-<span style="font-variant: small-caps;">l</span>-histidine), the active ingredient of chicken essence affects exercise-induced oxidative stress, cell integrity, and haematology biomarkers. In a randomized placebo-controlled repeated-measures design, ten healthy men ingested anserine in either a low dose (ANS-LD) 15 mg·kg<sup>−1</sup>·bw<sup>−1</sup>, high dose (ANS-HD) 30 mg·kg<sup>−1</sup>·bw<sup>−1</sup>, or placebo (PLA), following an exercise challenge (time to exhaustion), on three separate occasions. Anserine supplementation increased superoxide dismutase (SOD) by 50% (<i>p</i> < 0.001, effect size d = 0.8 for both ANS-LD and ANS-HD), and preserved catalase (CAT) activity suggesting an improved antioxidant activity. However, both ANS-LD and ANS-HD elevated glutathione disulfide (GSSG), (both <i>p</i> < 0.001, main treatment effect), and consequently lowered the glutathione to glutathione disulfide (GSH/GSSG) ratio compared with PLA (<i>p</i> < 0.01, main treatment effect), without significant effects on thiobarbituric acid active reactive substances (TBARS). Exercise-induced cell damage biomarkers of glutamic-oxaloacetic transaminase (GOT) and myoglobin were unaffected by anserine. There were slight but significant elevations in glutamate pyruvate transaminase (GPT) and creatine kinase isoenzyme (CKMB), especially in ANS-HD (<i>p</i> < 0.05) compared with ANS-LD or PLA. Haematological biomarkers were largely unaffected by anserine, its dose, and without interaction with post exercise time-course. However, compared with ANS-LD and PLA, ANS-HD increased the mean cell volume (MCV), and decreased the mean corpuscular haemoglobin concentration (MCHC) (<i>p</i> < 0.001). Anserine preserves cellular homoeostasis through enhanced antioxidant activity and protects cell integrity in healthy men, which is important for chronic disease prevention. However, anserine temporal elevated exercise-induced cell-damage, together with enhanced antioxidant activity and haematological responses suggest an augmented exercise-induced adaptative response and recovery.https://www.mdpi.com/2072-6643/12/4/1146anserinenutraceuticalexercise recoveryoxidative stresscell damagehaematocrit |
| spellingShingle | Ahmad Alkhatib Wen-Hsin Feng Yi-Jen Huang Chia-Hua Kuo Chien-Wen Hou Anserine Reverses Exercise-Induced Oxidative Stress and Preserves Cellular Homeostasis in Healthy Men Nutrients anserine nutraceutical exercise recovery oxidative stress cell damage haematocrit |
| title | Anserine Reverses Exercise-Induced Oxidative Stress and Preserves Cellular Homeostasis in Healthy Men |
| title_full | Anserine Reverses Exercise-Induced Oxidative Stress and Preserves Cellular Homeostasis in Healthy Men |
| title_fullStr | Anserine Reverses Exercise-Induced Oxidative Stress and Preserves Cellular Homeostasis in Healthy Men |
| title_full_unstemmed | Anserine Reverses Exercise-Induced Oxidative Stress and Preserves Cellular Homeostasis in Healthy Men |
| title_short | Anserine Reverses Exercise-Induced Oxidative Stress and Preserves Cellular Homeostasis in Healthy Men |
| title_sort | anserine reverses exercise induced oxidative stress and preserves cellular homeostasis in healthy men |
| topic | anserine nutraceutical exercise recovery oxidative stress cell damage haematocrit |
| url | https://www.mdpi.com/2072-6643/12/4/1146 |
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