Morphological and molecular analyses reveal two new species of Microcera (Nectriaceae, Hypocreales) associated with scale insects on walnut in China
The fungal genus Microcera consists of species mostly occurring as parasites of scale insects, but are also commonly isolated from soil or lichens. In the present study, we surveyed the diversity and assess the taxonomy of entomopathogenic fungi in Sichuan Province, China. Two new species of Microce...
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| Format: | Article |
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Pensoft Publishers
2023-05-01
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| Series: | MycoKeys |
| Online Access: | https://mycokeys.pensoft.net/article/103484/download/pdf/ |
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| author | Feng Liu Yu Deng Fei-Hu Wang Rajesh Jeewon Qian Zeng Xiu-Lan Xu Ying-Gao Liu Chun-Lin Yang |
| author_facet | Feng Liu Yu Deng Fei-Hu Wang Rajesh Jeewon Qian Zeng Xiu-Lan Xu Ying-Gao Liu Chun-Lin Yang |
| author_sort | Feng Liu |
| collection | DOAJ |
| description | The fungal genus Microcera consists of species mostly occurring as parasites of scale insects, but are also commonly isolated from soil or lichens. In the present study, we surveyed the diversity and assess the taxonomy of entomopathogenic fungi in Sichuan Province, China. Two new species of Microcera, viz. M. chrysomphaludis and M. pseudaulacaspidis, were isolated from scale insects colonising walnut (Juglans regia). Maximum Likelihood and Bayesian Inference analyses of ITS, LSU, tef1-α, rpb1, rpb2, acl1, act, tub2, cmdA and his3 sequence data provide evidence for the validity of the two species and their placement in Nectriaceae (Hypocreales). Microcera pseudaulacaspidis primarily differs from similar species by having more septate and smaller cylindrical macroconidia, as well as DNA sequence data. Meanwhile, Microcera chrysomphaludis has elliptical, one-septate ascospores with acute ends and cylindrical, slightly curved with 4–6 septate macroconidia up to 78 µm long. Morphological descriptions with illustrations of the novel species and DNA-based phylogeny generated from analyses of multigene dataset are also provided to better understand species relationships. |
| first_indexed | 2024-03-13T08:21:17Z |
| format | Article |
| id | doaj.art-0215b9c21b6640a8bbf86973b00fcbd2 |
| institution | Directory Open Access Journal |
| issn | 1314-4049 |
| language | English |
| last_indexed | 2024-03-13T08:21:17Z |
| publishDate | 2023-05-01 |
| publisher | Pensoft Publishers |
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| spelling | doaj.art-0215b9c21b6640a8bbf86973b00fcbd22023-05-31T08:11:08ZengPensoft PublishersMycoKeys1314-40492023-05-0198193510.3897/mycokeys.98.103484103484Morphological and molecular analyses reveal two new species of Microcera (Nectriaceae, Hypocreales) associated with scale insects on walnut in ChinaFeng Liu0Yu Deng1Fei-Hu Wang2Rajesh Jeewon3Qian Zeng4Xiu-Lan Xu5Ying-Gao Liu6Chun-Lin Yang7Sichuan Agricultural UniversitySichuan Agricultural UniversitySichuan Agricultural UniversityUniversity of MauritiusSichuan Agricultural UniversityForestry Research Institute, Chengdu Academy of Agricultural and Forestry SciencesSichuan Agricultural UniversitySichuan Agricultural UniversityThe fungal genus Microcera consists of species mostly occurring as parasites of scale insects, but are also commonly isolated from soil or lichens. In the present study, we surveyed the diversity and assess the taxonomy of entomopathogenic fungi in Sichuan Province, China. Two new species of Microcera, viz. M. chrysomphaludis and M. pseudaulacaspidis, were isolated from scale insects colonising walnut (Juglans regia). Maximum Likelihood and Bayesian Inference analyses of ITS, LSU, tef1-α, rpb1, rpb2, acl1, act, tub2, cmdA and his3 sequence data provide evidence for the validity of the two species and their placement in Nectriaceae (Hypocreales). Microcera pseudaulacaspidis primarily differs from similar species by having more septate and smaller cylindrical macroconidia, as well as DNA sequence data. Meanwhile, Microcera chrysomphaludis has elliptical, one-septate ascospores with acute ends and cylindrical, slightly curved with 4–6 septate macroconidia up to 78 µm long. Morphological descriptions with illustrations of the novel species and DNA-based phylogeny generated from analyses of multigene dataset are also provided to better understand species relationships.https://mycokeys.pensoft.net/article/103484/download/pdf/ |
| spellingShingle | Feng Liu Yu Deng Fei-Hu Wang Rajesh Jeewon Qian Zeng Xiu-Lan Xu Ying-Gao Liu Chun-Lin Yang Morphological and molecular analyses reveal two new species of Microcera (Nectriaceae, Hypocreales) associated with scale insects on walnut in China MycoKeys |
| title | Morphological and molecular analyses reveal two new species of Microcera (Nectriaceae, Hypocreales) associated with scale insects on walnut in China |
| title_full | Morphological and molecular analyses reveal two new species of Microcera (Nectriaceae, Hypocreales) associated with scale insects on walnut in China |
| title_fullStr | Morphological and molecular analyses reveal two new species of Microcera (Nectriaceae, Hypocreales) associated with scale insects on walnut in China |
| title_full_unstemmed | Morphological and molecular analyses reveal two new species of Microcera (Nectriaceae, Hypocreales) associated with scale insects on walnut in China |
| title_short | Morphological and molecular analyses reveal two new species of Microcera (Nectriaceae, Hypocreales) associated with scale insects on walnut in China |
| title_sort | morphological and molecular analyses reveal two new species of microcera nectriaceae hypocreales associated with scale insects on walnut in china |
| url | https://mycokeys.pensoft.net/article/103484/download/pdf/ |
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