Susceptibility of various cell lines to Neospora caninum tachyzoites cultivation
Neospora caninum is a coccidian protozoan parasite which is a major cause of bovine abortions and neonatal mortality in cattle, sheep, goat and horse. Occasionally, cultured cells are used for isolation and multiplication of the agent in vitro with several purposes. In this study the tachyzoite yiel...
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| Language: | English |
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Razi Vaccine and Serum Research Institute
2014-05-01
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| Series: | Archives of Razi Institute |
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| Online Access: | http://www.archrazi.com/browse.php?a_id=469&sid=1&slc_lang=en |
| _version_ | 1818525292027707392 |
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| author | Khordadmehr, M., Namavari, M., Khodakaram-Tafti, A. |
| author_facet | Khordadmehr, M., Namavari, M., Khodakaram-Tafti, A. |
| author_sort | Khordadmehr, M., |
| collection | DOAJ |
| description | Neospora caninum is a coccidian protozoan parasite which is a major cause of bovine abortions and neonatal mortality in cattle, sheep, goat and horse. Occasionally, cultured cells are used for isolation and multiplication of the agent in vitro with several purposes. In this study the tachyzoite yields of N. caninum were compared in various cell cultures as the host cell lines. Among the cell cultures tested, two presented good susceptibility to the agent: cell lines Vero and MA-104. SW742 and TLI (in vitro suspension culture of lymphoid cells infected with Theileria lestoquardi) showed moderate sensitivity. No viable tachyzoite were detected in the culture of MDCK and McCoy cell lines. These results demonstrate that MA-104 and SW742 cells present adequate susceptibility to N. caninum compared to Vero cells, which have been largely used to multiply the parasite in vitro. Moreover, these have easy manipulation, fast multiplication and relatively low nutritional requirements. In addition, the result of this study showed that TLI cell line as a suspension cell culture is susceptible to Nc-1 tachyzoites infection and could be used as an alternative host cell line for tachyzoites culture in vitro studies. |
| first_indexed | 2024-12-11T06:07:29Z |
| format | Article |
| id | doaj.art-0215cf80410a4dda8717b4a140c81d0b |
| institution | Directory Open Access Journal |
| issn | 0365-3439 2008-9872 |
| language | English |
| last_indexed | 2024-12-11T06:07:29Z |
| publishDate | 2014-05-01 |
| publisher | Razi Vaccine and Serum Research Institute |
| record_format | Article |
| series | Archives of Razi Institute |
| spelling | doaj.art-0215cf80410a4dda8717b4a140c81d0b2022-12-22T01:18:15ZengRazi Vaccine and Serum Research InstituteArchives of Razi Institute0365-34392008-98722014-05-016915762Susceptibility of various cell lines to Neospora caninum tachyzoites cultivationKhordadmehr, M., 0Namavari, M., 1Khodakaram-Tafti, A.2Department of Pathology, School of Veterinary Medicine, University of Shiraz, Shiraz, IranDepartment of Bacteriology, Razi Vaccine and Serum Research Institute, Shiraz, IranDepartment of Pathology, School of Veterinary Medicine, University of Shiraz, Shiraz, IranNeospora caninum is a coccidian protozoan parasite which is a major cause of bovine abortions and neonatal mortality in cattle, sheep, goat and horse. Occasionally, cultured cells are used for isolation and multiplication of the agent in vitro with several purposes. In this study the tachyzoite yields of N. caninum were compared in various cell cultures as the host cell lines. Among the cell cultures tested, two presented good susceptibility to the agent: cell lines Vero and MA-104. SW742 and TLI (in vitro suspension culture of lymphoid cells infected with Theileria lestoquardi) showed moderate sensitivity. No viable tachyzoite were detected in the culture of MDCK and McCoy cell lines. These results demonstrate that MA-104 and SW742 cells present adequate susceptibility to N. caninum compared to Vero cells, which have been largely used to multiply the parasite in vitro. Moreover, these have easy manipulation, fast multiplication and relatively low nutritional requirements. In addition, the result of this study showed that TLI cell line as a suspension cell culture is susceptible to Nc-1 tachyzoites infection and could be used as an alternative host cell line for tachyzoites culture in vitro studies.http://www.archrazi.com/browse.php?a_id=469&sid=1&slc_lang=enNeospora caninumVeroTLIMA-104SW742McCoyMDCK |
| spellingShingle | Khordadmehr, M., Namavari, M., Khodakaram-Tafti, A. Susceptibility of various cell lines to Neospora caninum tachyzoites cultivation Archives of Razi Institute Neospora caninum Vero TLI MA-104 SW742 McCoy MDCK |
| title | Susceptibility of various cell lines to Neospora caninum tachyzoites cultivation |
| title_full | Susceptibility of various cell lines to Neospora caninum tachyzoites cultivation |
| title_fullStr | Susceptibility of various cell lines to Neospora caninum tachyzoites cultivation |
| title_full_unstemmed | Susceptibility of various cell lines to Neospora caninum tachyzoites cultivation |
| title_short | Susceptibility of various cell lines to Neospora caninum tachyzoites cultivation |
| title_sort | susceptibility of various cell lines to neospora caninum tachyzoites cultivation |
| topic | Neospora caninum Vero TLI MA-104 SW742 McCoy MDCK |
| url | http://www.archrazi.com/browse.php?a_id=469&sid=1&slc_lang=en |
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