An in vitro model for caecal proteolytic fermentation potential of ingredients in broilers
Fermentation of protein in the caeca of chickens may lead to the production of potentially detrimental metabolites, which can reduce gut health. A poor precaecal digestion is expected to increase protein fermentation (PF), as more proteins are likely to enter the caeca. It is unknown if the undigest...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-04-01
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| Series: | Animal |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1751731123000642 |
| _version_ | 1797842749079158784 |
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| author | M.L. Elling-Staats A.K. Kies J.W. Cone W.F. Pellikaan R.P. Kwakkel |
| author_facet | M.L. Elling-Staats A.K. Kies J.W. Cone W.F. Pellikaan R.P. Kwakkel |
| author_sort | M.L. Elling-Staats |
| collection | DOAJ |
| description | Fermentation of protein in the caeca of chickens may lead to the production of potentially detrimental metabolites, which can reduce gut health. A poor precaecal digestion is expected to increase protein fermentation (PF), as more proteins are likely to enter the caeca. It is unknown if the undigested protein that enters the caeca differs in fermentability depending on their ingredient source. In order to predict which feed ingredients increase the risk of PF, an in vitro procedure was developed, which simulates the gastric and enteric digestion, subsequent caecal fermentation. After digestion, amino acids and peptides smaller than 3.5 kD in the soluble fraction were removed by means of dialysis. These amino acids and peptides are assumed to be hydrolysed and absorbed in the small intestine of poultry and therefore not used in the fermentation assay. The remaining soluble and fine digesta fractions were inoculated with caecal microbes. In chicken, the soluble and fine fractions enter the caeca, to be fermented, while insoluble and coarse fractions bypass them. The inoculum was made N-free to ensure bacteria would require the N from the digesta fractions for their growth and activity. The gas production (GP) from the inoculum, therefore, reflected the ability of bacteria to use N from substrates and was an indirect measure for PF. The Maximum GP rate of ingredients averaged 21.3 ± 0.9 ml/h (mean ± SEM) and was in some cases more rapid than the positive control (urea, maximum GP rate = 16.5 ml/h). Only small differences in GP kinetics were found between protein ingredients. Branched-chain fatty acids and ammonia concentrations in the fermentation fluid after 24 hours showed no differences between ingredients. Results indicate that solubilised undigested proteins larger than 3.5 kD are rapidly fermented independent of its source when an equal amount of N is present. |
| first_indexed | 2024-04-09T16:53:05Z |
| format | Article |
| id | doaj.art-0217f348ab344e2a9ea280d82f347646 |
| institution | Directory Open Access Journal |
| issn | 1751-7311 |
| language | English |
| last_indexed | 2024-04-09T16:53:05Z |
| publishDate | 2023-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Animal |
| spelling | doaj.art-0217f348ab344e2a9ea280d82f3476462023-04-21T06:45:07ZengElsevierAnimal1751-73112023-04-01174100768An in vitro model for caecal proteolytic fermentation potential of ingredients in broilersM.L. Elling-Staats0A.K. Kies1J.W. Cone2W.F. Pellikaan3R.P. Kwakkel4Animal Nutrition Group, Department of Animal Sciences, Wageningen University, PO Box 338, 6700 AH Wageningen, the Netherlands; Corresponding author.DSM Nutritional Products, Animal Nutrition and Health – EMEA, Kaiseraugst, SwitzerlandAnimal Nutrition Group, Department of Animal Sciences, Wageningen University, PO Box 338, 6700 AH Wageningen, the NetherlandsAnimal Nutrition Group, Department of Animal Sciences, Wageningen University, PO Box 338, 6700 AH Wageningen, the NetherlandsAnimal Nutrition Group, Department of Animal Sciences, Wageningen University, PO Box 338, 6700 AH Wageningen, the NetherlandsFermentation of protein in the caeca of chickens may lead to the production of potentially detrimental metabolites, which can reduce gut health. A poor precaecal digestion is expected to increase protein fermentation (PF), as more proteins are likely to enter the caeca. It is unknown if the undigested protein that enters the caeca differs in fermentability depending on their ingredient source. In order to predict which feed ingredients increase the risk of PF, an in vitro procedure was developed, which simulates the gastric and enteric digestion, subsequent caecal fermentation. After digestion, amino acids and peptides smaller than 3.5 kD in the soluble fraction were removed by means of dialysis. These amino acids and peptides are assumed to be hydrolysed and absorbed in the small intestine of poultry and therefore not used in the fermentation assay. The remaining soluble and fine digesta fractions were inoculated with caecal microbes. In chicken, the soluble and fine fractions enter the caeca, to be fermented, while insoluble and coarse fractions bypass them. The inoculum was made N-free to ensure bacteria would require the N from the digesta fractions for their growth and activity. The gas production (GP) from the inoculum, therefore, reflected the ability of bacteria to use N from substrates and was an indirect measure for PF. The Maximum GP rate of ingredients averaged 21.3 ± 0.9 ml/h (mean ± SEM) and was in some cases more rapid than the positive control (urea, maximum GP rate = 16.5 ml/h). Only small differences in GP kinetics were found between protein ingredients. Branched-chain fatty acids and ammonia concentrations in the fermentation fluid after 24 hours showed no differences between ingredients. Results indicate that solubilised undigested proteins larger than 3.5 kD are rapidly fermented independent of its source when an equal amount of N is present.http://www.sciencedirect.com/science/article/pii/S1751731123000642ChickenDialysisIn vitro digestionIn vitro gas production techniqueProtein fermentation |
| spellingShingle | M.L. Elling-Staats A.K. Kies J.W. Cone W.F. Pellikaan R.P. Kwakkel An in vitro model for caecal proteolytic fermentation potential of ingredients in broilers Animal Chicken Dialysis In vitro digestion In vitro gas production technique Protein fermentation |
| title | An in vitro model for caecal proteolytic fermentation potential of ingredients in broilers |
| title_full | An in vitro model for caecal proteolytic fermentation potential of ingredients in broilers |
| title_fullStr | An in vitro model for caecal proteolytic fermentation potential of ingredients in broilers |
| title_full_unstemmed | An in vitro model for caecal proteolytic fermentation potential of ingredients in broilers |
| title_short | An in vitro model for caecal proteolytic fermentation potential of ingredients in broilers |
| title_sort | in vitro model for caecal proteolytic fermentation potential of ingredients in broilers |
| topic | Chicken Dialysis In vitro digestion In vitro gas production technique Protein fermentation |
| url | http://www.sciencedirect.com/science/article/pii/S1751731123000642 |
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