Molecular and phylogenetic analysis of HIV-1 variants circulating among injecting drug users in Mashhad-Iran
<p>Abstract</p> <p>Genetic and phylogenetic information on the HIV-1 epidemic in Middle-East Countries, and in particular in Iran, are extremely limited. By March 2004, the Iranian Ministry of Health officially reported a cumulative number of 6'532 HIV positive individuals and...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2006-09-01
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| Series: | Infectious Agents and Cancer |
| Online Access: | http://www.infectagentscancer.com/content/1/1/4 |
| _version_ | 1811266684010364928 |
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| author | Buonaguro FM Rezza G Farid R Ciccozzi M Tornesello ML Tagliamonte M Naderi HR Buonaguro L |
| author_facet | Buonaguro FM Rezza G Farid R Ciccozzi M Tornesello ML Tagliamonte M Naderi HR Buonaguro L |
| author_sort | Buonaguro FM |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Genetic and phylogenetic information on the HIV-1 epidemic in Middle-East Countries, and in particular in Iran, are extremely limited. By March 2004, the Iranian Ministry of Health officially reported a cumulative number of 6'532 HIV positive individuals and 214 AIDS cases in the Iranian HIV-1 epidemic. The intra-venous drug users (IDUs) represent the group at highest risk for HIV-1 infection in Iran, accounting for almost 63% of all HIV-infected population. In this regards, a molecular phylogenetic study has been performed on a sentinel cohort of HIV-1 seropositive IDUs enrolled at the end of 2005 at the University of Mashhad, the largest city North East of Tehran. The study has been performed on both <it>gag </it>and <it>env </it>subgenomic regions amplified by Polymerase Chain Reaction (PCR) from peripheral blood mononuclear cells (PBMCs) and characterized by direct DNA sequence analysis. The results reported here show that the HIV-1 subtype A is circulating in this IDUs sentinel cohort. Moreover, the single phylogenetic cluster as well as the <it>intra</it>-group low nucleotide divergence is indicative of a recent outbreak. Unexpectedly, the Iranian samples appear to be phylogenetically derived from African Sub-Saharan subtype A viruses, raising stirring speculations on HIV-1 introduction into the IDUs epidemic in Mashhad. This sentinel study could represent the starting point for a wider molecular survey of the HIV-1 epidemics in Iran to evaluate in detail the distribution of genetic subtypes and possible natural drug-resistant variants, which are extremely helpful information to design diagnostic and therapeutic strategies.</p> |
| first_indexed | 2024-04-12T20:47:42Z |
| format | Article |
| id | doaj.art-02185d2a91df483bafd7e274a2a004d1 |
| institution | Directory Open Access Journal |
| issn | 1750-9378 |
| language | English |
| last_indexed | 2024-04-12T20:47:42Z |
| publishDate | 2006-09-01 |
| publisher | BMC |
| record_format | Article |
| series | Infectious Agents and Cancer |
| spelling | doaj.art-02185d2a91df483bafd7e274a2a004d12022-12-22T03:17:12ZengBMCInfectious Agents and Cancer1750-93782006-09-0111410.1186/1750-9378-1-4Molecular and phylogenetic analysis of HIV-1 variants circulating among injecting drug users in Mashhad-IranBuonaguro FMRezza GFarid RCiccozzi MTornesello MLTagliamonte MNaderi HRBuonaguro L<p>Abstract</p> <p>Genetic and phylogenetic information on the HIV-1 epidemic in Middle-East Countries, and in particular in Iran, are extremely limited. By March 2004, the Iranian Ministry of Health officially reported a cumulative number of 6'532 HIV positive individuals and 214 AIDS cases in the Iranian HIV-1 epidemic. The intra-venous drug users (IDUs) represent the group at highest risk for HIV-1 infection in Iran, accounting for almost 63% of all HIV-infected population. In this regards, a molecular phylogenetic study has been performed on a sentinel cohort of HIV-1 seropositive IDUs enrolled at the end of 2005 at the University of Mashhad, the largest city North East of Tehran. The study has been performed on both <it>gag </it>and <it>env </it>subgenomic regions amplified by Polymerase Chain Reaction (PCR) from peripheral blood mononuclear cells (PBMCs) and characterized by direct DNA sequence analysis. The results reported here show that the HIV-1 subtype A is circulating in this IDUs sentinel cohort. Moreover, the single phylogenetic cluster as well as the <it>intra</it>-group low nucleotide divergence is indicative of a recent outbreak. Unexpectedly, the Iranian samples appear to be phylogenetically derived from African Sub-Saharan subtype A viruses, raising stirring speculations on HIV-1 introduction into the IDUs epidemic in Mashhad. This sentinel study could represent the starting point for a wider molecular survey of the HIV-1 epidemics in Iran to evaluate in detail the distribution of genetic subtypes and possible natural drug-resistant variants, which are extremely helpful information to design diagnostic and therapeutic strategies.</p>http://www.infectagentscancer.com/content/1/1/4 |
| spellingShingle | Buonaguro FM Rezza G Farid R Ciccozzi M Tornesello ML Tagliamonte M Naderi HR Buonaguro L Molecular and phylogenetic analysis of HIV-1 variants circulating among injecting drug users in Mashhad-Iran Infectious Agents and Cancer |
| title | Molecular and phylogenetic analysis of HIV-1 variants circulating among injecting drug users in Mashhad-Iran |
| title_full | Molecular and phylogenetic analysis of HIV-1 variants circulating among injecting drug users in Mashhad-Iran |
| title_fullStr | Molecular and phylogenetic analysis of HIV-1 variants circulating among injecting drug users in Mashhad-Iran |
| title_full_unstemmed | Molecular and phylogenetic analysis of HIV-1 variants circulating among injecting drug users in Mashhad-Iran |
| title_short | Molecular and phylogenetic analysis of HIV-1 variants circulating among injecting drug users in Mashhad-Iran |
| title_sort | molecular and phylogenetic analysis of hiv 1 variants circulating among injecting drug users in mashhad iran |
| url | http://www.infectagentscancer.com/content/1/1/4 |
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