Synthesis, Characterization and Cytotoxicity Studies of Aminated Microcrystalline Cellulose Derivatives against Melanoma and Breast Cancer Cell Lines
Cellulose based materials are emerging in the commercial fields and high-end applications, especially in biomedicines. Aminated cellulose derivatives have been extensively used for various applications but limited data are available regarding its cytotoxicity studies for biomedical application. The...
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| Format: | Article |
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MDPI AG
2020-11-01
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| Series: | Polymers |
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| Online Access: | https://www.mdpi.com/2073-4360/12/11/2634 |
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| author | Farzana Nazir Mudassir Iqbal |
| author_facet | Farzana Nazir Mudassir Iqbal |
| author_sort | Farzana Nazir |
| collection | DOAJ |
| description | Cellulose based materials are emerging in the commercial fields and high-end applications, especially in biomedicines. Aminated cellulose derivatives have been extensively used for various applications but limited data are available regarding its cytotoxicity studies for biomedical application. The aim of this study is to synthesize different 6-deoxy-amino-cellulose derivatives from Microcrystalline cellulose (MCC) via tosylation and explore their cytotoxic potential against normal fibroblasts, melanoma and breast cancer. 6-deoxy-6-hydrazide Cellulose (Cell Hyd) 6-deoxy-6-diethylamide Cellulose (Cell DEA) and 6-deoxy-6-diethyltriamine Cellulose (Cell DETA) were prepared and characterized by various technologies like Fourier transform infrared spectroscopy-attenuated total reflectance (FTIR-ATR), nuclear magnetic resonance spectroscopy (NMR), X-ray diffractogram (XRD), Scanning Electron microscopy (SEM), Elemental Analysis and Zeta potential measurements. Cytotoxicity was evaluated against normal fibroblasts (NIH3T3), mouse skin melanoma (B16F10), human epithelial adenocarcinoma (MDA-MB-231) and human breast adenocarcinoma (MCF-7) cell lines. IC<sub>50</sub> values obtained from cytotoxicity assay and live/dead assay images analysis showed MCC was non cytotoxic while Cell Hyd, Cell DEA and Cell DETA exhibited noncytotoxic activity up to 200 μg/mL to normal fibroblast cells NIH3T3, suggesting its safe use in medical fields. The mouse skin melanoma (B16F10) are the most sensitive cells to the cytotoxic effects of Cell Hyd, Cell DEA and Cell DETA, followed by human breast adenocarcinoma (MCF-7). Based on our study, it is suggested that aminated cellulose derivatives could be promising candidates for tissue engineering applications and in cancer inhibiting studies in future. |
| first_indexed | 2024-03-10T14:58:28Z |
| format | Article |
| id | doaj.art-021d744926be4e75a8ac01caf39b8ed2 |
| institution | Directory Open Access Journal |
| issn | 2073-4360 |
| language | English |
| last_indexed | 2024-03-10T14:58:28Z |
| publishDate | 2020-11-01 |
| publisher | MDPI AG |
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| series | Polymers |
| spelling | doaj.art-021d744926be4e75a8ac01caf39b8ed22023-11-20T20:22:26ZengMDPI AGPolymers2073-43602020-11-011211263410.3390/polym12112634Synthesis, Characterization and Cytotoxicity Studies of Aminated Microcrystalline Cellulose Derivatives against Melanoma and Breast Cancer Cell LinesFarzana Nazir0Mudassir Iqbal1Department of Chemistry, School of Natural Sciences, National University of Sciences and Technology (NUST), Islamabad 44000, PakistanDepartment of Chemistry, School of Natural Sciences, National University of Sciences and Technology (NUST), Islamabad 44000, PakistanCellulose based materials are emerging in the commercial fields and high-end applications, especially in biomedicines. Aminated cellulose derivatives have been extensively used for various applications but limited data are available regarding its cytotoxicity studies for biomedical application. The aim of this study is to synthesize different 6-deoxy-amino-cellulose derivatives from Microcrystalline cellulose (MCC) via tosylation and explore their cytotoxic potential against normal fibroblasts, melanoma and breast cancer. 6-deoxy-6-hydrazide Cellulose (Cell Hyd) 6-deoxy-6-diethylamide Cellulose (Cell DEA) and 6-deoxy-6-diethyltriamine Cellulose (Cell DETA) were prepared and characterized by various technologies like Fourier transform infrared spectroscopy-attenuated total reflectance (FTIR-ATR), nuclear magnetic resonance spectroscopy (NMR), X-ray diffractogram (XRD), Scanning Electron microscopy (SEM), Elemental Analysis and Zeta potential measurements. Cytotoxicity was evaluated against normal fibroblasts (NIH3T3), mouse skin melanoma (B16F10), human epithelial adenocarcinoma (MDA-MB-231) and human breast adenocarcinoma (MCF-7) cell lines. IC<sub>50</sub> values obtained from cytotoxicity assay and live/dead assay images analysis showed MCC was non cytotoxic while Cell Hyd, Cell DEA and Cell DETA exhibited noncytotoxic activity up to 200 μg/mL to normal fibroblast cells NIH3T3, suggesting its safe use in medical fields. The mouse skin melanoma (B16F10) are the most sensitive cells to the cytotoxic effects of Cell Hyd, Cell DEA and Cell DETA, followed by human breast adenocarcinoma (MCF-7). Based on our study, it is suggested that aminated cellulose derivatives could be promising candidates for tissue engineering applications and in cancer inhibiting studies in future.https://www.mdpi.com/2073-4360/12/11/2634celluloseMCCtosylated celluloseaminated cellulosecytotoxicitymelanoma |
| spellingShingle | Farzana Nazir Mudassir Iqbal Synthesis, Characterization and Cytotoxicity Studies of Aminated Microcrystalline Cellulose Derivatives against Melanoma and Breast Cancer Cell Lines Polymers cellulose MCC tosylated cellulose aminated cellulose cytotoxicity melanoma |
| title | Synthesis, Characterization and Cytotoxicity Studies of Aminated Microcrystalline Cellulose Derivatives against Melanoma and Breast Cancer Cell Lines |
| title_full | Synthesis, Characterization and Cytotoxicity Studies of Aminated Microcrystalline Cellulose Derivatives against Melanoma and Breast Cancer Cell Lines |
| title_fullStr | Synthesis, Characterization and Cytotoxicity Studies of Aminated Microcrystalline Cellulose Derivatives against Melanoma and Breast Cancer Cell Lines |
| title_full_unstemmed | Synthesis, Characterization and Cytotoxicity Studies of Aminated Microcrystalline Cellulose Derivatives against Melanoma and Breast Cancer Cell Lines |
| title_short | Synthesis, Characterization and Cytotoxicity Studies of Aminated Microcrystalline Cellulose Derivatives against Melanoma and Breast Cancer Cell Lines |
| title_sort | synthesis characterization and cytotoxicity studies of aminated microcrystalline cellulose derivatives against melanoma and breast cancer cell lines |
| topic | cellulose MCC tosylated cellulose aminated cellulose cytotoxicity melanoma |
| url | https://www.mdpi.com/2073-4360/12/11/2634 |
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