Staphylococcal Biofilm on the Surface of Catheters: Electron Microscopy Evaluation of the Inhibition of Biofilm Growth by RNAIII Inhibiting Peptide
<i>Staphylococcus aureus</i> and coagulase-negative staphylococci (CoNS) have become the main causative agents of medical device-related infections due to their biofilm-forming capability, which protects them from the host’s immune system and from the action of antimicrobials. This study...
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| Format: | Article |
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MDPI AG
2021-07-01
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| Series: | Antibiotics |
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| Online Access: | https://www.mdpi.com/2079-6382/10/7/879 |
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| author | Adilson de Oliveira Luiza Pinheiro-Hubinger Valéria Cataneli Pereira Danilo Flávio Moraes Riboli Katheryne Benini Martins Letícia Calixto Romero Maria de Lourdes Ribeiro de Souza da Cunha |
| author_facet | Adilson de Oliveira Luiza Pinheiro-Hubinger Valéria Cataneli Pereira Danilo Flávio Moraes Riboli Katheryne Benini Martins Letícia Calixto Romero Maria de Lourdes Ribeiro de Souza da Cunha |
| author_sort | Adilson de Oliveira |
| collection | DOAJ |
| description | <i>Staphylococcus aureus</i> and coagulase-negative staphylococci (CoNS) have become the main causative agents of medical device-related infections due to their biofilm-forming capability, which protects them from the host’s immune system and from the action of antimicrobials. This study evaluated the ability of RNA III inhibiting peptide (RIP) to inhibit biofilm formation in 10 strains isolated from clinical materials, including one <i>S. aureus</i> strain, two <i>S. epidermidis</i>, two <i>S. haemolyticus</i>, two <i>S. lugdunensis</i>, and one isolate each of the following species: <i>S. warneri</i>, <i>S. hominis</i>, and <i>S. saprophyticus</i>. The isolates were selected from a total of 200 strains evaluated regarding phenotypic biofilm production and the presence and expression of the <i>ica</i> operon. The isolates were cultured in trypticase soy broth with 2% glucose in 96-well polystyrene plates containing catheter segments in the presence and absence of RIP. The catheter segments were observed by scanning electron microscopy. The results showed inhibition of biofilm formation in the presence of RIP in all CoNS isolates; however, RIP did not interfere with biofilm formation by <i>S. aureus</i>. RIP is a promising tool that might be used in the future for the prevention of biofilm-related infections caused by CoNS. |
| first_indexed | 2024-03-10T09:47:32Z |
| format | Article |
| id | doaj.art-021d8e6d76b54d4ab6712b64c963a729 |
| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-10T09:47:32Z |
| publishDate | 2021-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-021d8e6d76b54d4ab6712b64c963a7292023-11-22T03:04:43ZengMDPI AGAntibiotics2079-63822021-07-0110787910.3390/antibiotics10070879Staphylococcal Biofilm on the Surface of Catheters: Electron Microscopy Evaluation of the Inhibition of Biofilm Growth by RNAIII Inhibiting PeptideAdilson de Oliveira0Luiza Pinheiro-Hubinger1Valéria Cataneli Pereira2Danilo Flávio Moraes Riboli3Katheryne Benini Martins4Letícia Calixto Romero5Maria de Lourdes Ribeiro de Souza da Cunha6Department of Chemical and Biological Sciences, Biosciences Institute, UNESP—Universidade Estadual Paulista, Botucatu 18618-691, BrazilDepartment of Anatomic Pathology, Lauro de Souza Lima Institute, Bauru 17034-971, BrazilDepartment of Chemical and Biological Sciences, Biosciences Institute, UNESP—Universidade Estadual Paulista, Botucatu 18618-691, BrazilDepartment of Chemical and Biological Sciences, Biosciences Institute, UNESP—Universidade Estadual Paulista, Botucatu 18618-691, BrazilDepartment of Chemical and Biological Sciences, Biosciences Institute, UNESP—Universidade Estadual Paulista, Botucatu 18618-691, BrazilDepartment of Chemical and Biological Sciences, Biosciences Institute, UNESP—Universidade Estadual Paulista, Botucatu 18618-691, BrazilDepartment of Chemical and Biological Sciences, Biosciences Institute, UNESP—Universidade Estadual Paulista, Botucatu 18618-691, Brazil<i>Staphylococcus aureus</i> and coagulase-negative staphylococci (CoNS) have become the main causative agents of medical device-related infections due to their biofilm-forming capability, which protects them from the host’s immune system and from the action of antimicrobials. This study evaluated the ability of RNA III inhibiting peptide (RIP) to inhibit biofilm formation in 10 strains isolated from clinical materials, including one <i>S. aureus</i> strain, two <i>S. epidermidis</i>, two <i>S. haemolyticus</i>, two <i>S. lugdunensis</i>, and one isolate each of the following species: <i>S. warneri</i>, <i>S. hominis</i>, and <i>S. saprophyticus</i>. The isolates were selected from a total of 200 strains evaluated regarding phenotypic biofilm production and the presence and expression of the <i>ica</i> operon. The isolates were cultured in trypticase soy broth with 2% glucose in 96-well polystyrene plates containing catheter segments in the presence and absence of RIP. The catheter segments were observed by scanning electron microscopy. The results showed inhibition of biofilm formation in the presence of RIP in all CoNS isolates; however, RIP did not interfere with biofilm formation by <i>S. aureus</i>. RIP is a promising tool that might be used in the future for the prevention of biofilm-related infections caused by CoNS.https://www.mdpi.com/2079-6382/10/7/879<i>Staphylococcus aureus</i>coagulase-negative staphylococcibiofilm structurequorum sensingcatheterRIP |
| spellingShingle | Adilson de Oliveira Luiza Pinheiro-Hubinger Valéria Cataneli Pereira Danilo Flávio Moraes Riboli Katheryne Benini Martins Letícia Calixto Romero Maria de Lourdes Ribeiro de Souza da Cunha Staphylococcal Biofilm on the Surface of Catheters: Electron Microscopy Evaluation of the Inhibition of Biofilm Growth by RNAIII Inhibiting Peptide Antibiotics <i>Staphylococcus aureus</i> coagulase-negative staphylococci biofilm structure quorum sensing catheter RIP |
| title | Staphylococcal Biofilm on the Surface of Catheters: Electron Microscopy Evaluation of the Inhibition of Biofilm Growth by RNAIII Inhibiting Peptide |
| title_full | Staphylococcal Biofilm on the Surface of Catheters: Electron Microscopy Evaluation of the Inhibition of Biofilm Growth by RNAIII Inhibiting Peptide |
| title_fullStr | Staphylococcal Biofilm on the Surface of Catheters: Electron Microscopy Evaluation of the Inhibition of Biofilm Growth by RNAIII Inhibiting Peptide |
| title_full_unstemmed | Staphylococcal Biofilm on the Surface of Catheters: Electron Microscopy Evaluation of the Inhibition of Biofilm Growth by RNAIII Inhibiting Peptide |
| title_short | Staphylococcal Biofilm on the Surface of Catheters: Electron Microscopy Evaluation of the Inhibition of Biofilm Growth by RNAIII Inhibiting Peptide |
| title_sort | staphylococcal biofilm on the surface of catheters electron microscopy evaluation of the inhibition of biofilm growth by rnaiii inhibiting peptide |
| topic | <i>Staphylococcus aureus</i> coagulase-negative staphylococci biofilm structure quorum sensing catheter RIP |
| url | https://www.mdpi.com/2079-6382/10/7/879 |
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