rMELEISH: A Novel Recombinant Multiepitope-Based Protein Applied to the Serodiagnosis of Both Canine and Human Visceral Leishmaniasis
Background: visceral leishmaniasis (VL) is a critical public health problem in over ninety countries. The control measures adopted in Brazil have been insufficient when it comes to preventing the spread of this overlooked disease. In this context, a precise diagnosis of VL in dogs and humans could h...
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MDPI AG
2023-02-01
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| Series: | Pathogens |
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| Online Access: | https://www.mdpi.com/2076-0817/12/2/302 |
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| author | Daniel Silva Dias Juliana Martins Machado Patrícia Aparecida Fernandes Ribeiro Amanda Sanchez Machado Fernanda Fonseca Ramos Lais Moreira Nogueira Ana Alice Maia Gonçalves Luana de Sousa Ramos Isadora Braga Gandra Flaviane Silva Coutinho Michelli dos Santos Jonatas Oliveira da Silva Miguel Angel Chávez-Fumagalli Rafael Gonçalves Teixeira-Neto Ana Thereza Chaves Mariana Campos-da-Paz Amanda A. Souza Rodolfo Cordeiro Giunchetti Sonia Maria Freitas Sandra Lyon Danielle Ferreira de Magalhães-Soares Julia Angelica Gonçalves Silveira Eduardo Sergio Silva Eduardo Antonio Ferraz Coelho Alexsandro Sobreira Galdino |
| author_facet | Daniel Silva Dias Juliana Martins Machado Patrícia Aparecida Fernandes Ribeiro Amanda Sanchez Machado Fernanda Fonseca Ramos Lais Moreira Nogueira Ana Alice Maia Gonçalves Luana de Sousa Ramos Isadora Braga Gandra Flaviane Silva Coutinho Michelli dos Santos Jonatas Oliveira da Silva Miguel Angel Chávez-Fumagalli Rafael Gonçalves Teixeira-Neto Ana Thereza Chaves Mariana Campos-da-Paz Amanda A. Souza Rodolfo Cordeiro Giunchetti Sonia Maria Freitas Sandra Lyon Danielle Ferreira de Magalhães-Soares Julia Angelica Gonçalves Silveira Eduardo Sergio Silva Eduardo Antonio Ferraz Coelho Alexsandro Sobreira Galdino |
| author_sort | Daniel Silva Dias |
| collection | DOAJ |
| description | Background: visceral leishmaniasis (VL) is a critical public health problem in over ninety countries. The control measures adopted in Brazil have been insufficient when it comes to preventing the spread of this overlooked disease. In this context, a precise diagnosis of VL in dogs and humans could help to reduce the number of cases of this disease. Distinct studies for the diagnosis of VL have used single recombinant proteins in serological assays; however, the results have been variable, mainly in relation to the sensitivity of the antigens. In this context, the development of multiepitope-based proteins could be relevant to solving such problem. Methods: a chimeric protein (rMELEISH) was constructed based on amino acid sequences from kinesin 39 (k39), alpha-tubulin, and heat-shock proteins HSP70 and HSP 83.1, and tested in enzyme-linked immunosorbent (ELISA) for the detection of <i>L. infantum</i> infection using canine (<i>n</i> = 140) and human (<i>n</i> = 145) sera samples. Results: in the trials, rMELEISH was able to discriminate between VL cases and cross-reactive diseases and healthy samples, with sensitivity and specificity values of 100%, as compared to the use of a soluble Leishmania antigenic extract (SLA). Conclusions: the preliminary data suggest that rMELEISH has the potential to be tested in future studies against a larger serological panel and in field conditions for the diagnosis of canine and human VL. |
| first_indexed | 2024-03-11T08:18:31Z |
| format | Article |
| id | doaj.art-021ea002caec47a7bcff97acb8556a97 |
| institution | Directory Open Access Journal |
| issn | 2076-0817 |
| language | English |
| last_indexed | 2024-03-11T08:18:31Z |
| publishDate | 2023-02-01 |
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| series | Pathogens |
| spelling | doaj.art-021ea002caec47a7bcff97acb8556a972023-11-16T22:34:46ZengMDPI AGPathogens2076-08172023-02-0112230210.3390/pathogens12020302rMELEISH: A Novel Recombinant Multiepitope-Based Protein Applied to the Serodiagnosis of Both Canine and Human Visceral LeishmaniasisDaniel Silva Dias0Juliana Martins Machado1Patrícia Aparecida Fernandes Ribeiro2Amanda Sanchez Machado3Fernanda Fonseca Ramos4Lais Moreira Nogueira5Ana Alice Maia Gonçalves6Luana de Sousa Ramos7Isadora Braga Gandra8Flaviane Silva Coutinho9Michelli dos Santos10Jonatas Oliveira da Silva11Miguel Angel Chávez-Fumagalli12Rafael Gonçalves Teixeira-Neto13Ana Thereza Chaves14Mariana Campos-da-Paz15Amanda A. Souza16Rodolfo Cordeiro Giunchetti17Sonia Maria Freitas18Sandra Lyon19Danielle Ferreira de Magalhães-Soares20Julia Angelica Gonçalves Silveira21Eduardo Sergio Silva22Eduardo Antonio Ferraz Coelho23Alexsandro Sobreira Galdino24Laboratório de Biotecnologia de Microrganismos, Universidade Federal de São João Del-Rei (UFSJ), Campus Centro Oeste, Divinópolis 35501-296, MG, BrazilLaboratório de Biotecnologia de Microrganismos, Universidade Federal de São João Del-Rei (UFSJ), Campus Centro Oeste, Divinópolis 35501-296, MG, BrazilLaboratório de Biotecnologia de Microrganismos, Universidade Federal de São João Del-Rei (UFSJ), Campus Centro Oeste, Divinópolis 35501-296, MG, BrazilPrograma de Pós-Graduação em Ciências da Saúde: Infectologia e Medicina Tropical, Faculdade de Medicina, Universidade Federal de Minas Gerais, Av. Prof. Alfredo Balena, 190, Belo Horizonte 30130-100, MG, BrazilPrograma de Pós-Graduação em Ciências da Saúde: Infectologia e Medicina Tropical, Faculdade de Medicina, Universidade Federal de Minas Gerais, Av. Prof. Alfredo Balena, 190, Belo Horizonte 30130-100, MG, BrazilLaboratório de Biotecnologia de Microrganismos, Universidade Federal de São João Del-Rei (UFSJ), Campus Centro Oeste, Divinópolis 35501-296, MG, BrazilLaboratório de Biologia das Interações Celulares, Departamento de Morfologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte 31270-901, MG, BrazilLaboratório de Biotecnologia de Microrganismos, Universidade Federal de São João Del-Rei (UFSJ), Campus Centro Oeste, Divinópolis 35501-296, MG, BrazilLaboratório de Biotecnologia de Microrganismos, Universidade Federal de São João Del-Rei (UFSJ), Campus Centro Oeste, Divinópolis 35501-296, MG, BrazilLaboratório de Biotecnologia de Microrganismos, Universidade Federal de São João Del-Rei (UFSJ), Campus Centro Oeste, Divinópolis 35501-296, MG, BrazilLaboratório de Biotecnologia de Microrganismos, Universidade Federal de São João Del-Rei (UFSJ), Campus Centro Oeste, Divinópolis 35501-296, MG, BrazilLaboratório de Biotecnologia de Microrganismos, Universidade Federal de São João Del-Rei (UFSJ), Campus Centro Oeste, Divinópolis 35501-296, MG, BrazilComputational Biology and Chemistry Research Group, Vicerrectorado de Investigación, Universidad Católica de Santa María, Urb. San José S/N, Arequipa 04000, PeruLaboratório de Doenças Infecto-Parasitárias, Universidade Federal de São João Del-Rei, Divinópolis 35501-296, MG, BrazilPrograma de Pós-Graduação em Ciências da Saúde: Infectologia e Medicina Tropical, Faculdade de Medicina, Universidade Federal de Minas Gerais, Av. Prof. Alfredo Balena, 190, Belo Horizonte 30130-100, MG, BrazilLaboratório de Bioativos & Nanobiotecnologia, Universidade Federal de São João Del-Rei, Divinópolis 35501-296, MG, BrazilLaboratório Nacional de Biociências (LNBio), Centro Nacional de Pesquisa em Energia e Materiais, Campinas 13083-970, SP, BrazilLaboratório de Biologia das Interações Celulares, Departamento de Morfologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte 31270-901, MG, BrazilLaboratorio de Biofísica, Instituto de Biologia, University of Brasilia, Brasília 70910-900, DF, BrazilFundação Hospitalar do Estado de Minas Gerais, Hospital Eduardo de Menezes, Belo Horizonte 30622-020, MG, BrazilDepartamento de Medicina Veterinária Preventiva, Escola de Veterinária, Universidade Federal de Minas Gerais, Belo Horizonte 31270-901, MG, BrazilDepartamento de Medicina Veterinária Preventiva, Escola de Veterinária, Universidade Federal de Minas Gerais, Belo Horizonte 31270-901, MG, BrazilLaboratório de Doenças Infecto-Parasitárias, Universidade Federal de São João Del-Rei, Divinópolis 35501-296, MG, BrazilPrograma de Pós-Graduação em Ciências da Saúde: Infectologia e Medicina Tropical, Faculdade de Medicina, Universidade Federal de Minas Gerais, Av. Prof. Alfredo Balena, 190, Belo Horizonte 30130-100, MG, BrazilLaboratório de Biotecnologia de Microrganismos, Universidade Federal de São João Del-Rei (UFSJ), Campus Centro Oeste, Divinópolis 35501-296, MG, BrazilBackground: visceral leishmaniasis (VL) is a critical public health problem in over ninety countries. The control measures adopted in Brazil have been insufficient when it comes to preventing the spread of this overlooked disease. In this context, a precise diagnosis of VL in dogs and humans could help to reduce the number of cases of this disease. Distinct studies for the diagnosis of VL have used single recombinant proteins in serological assays; however, the results have been variable, mainly in relation to the sensitivity of the antigens. In this context, the development of multiepitope-based proteins could be relevant to solving such problem. Methods: a chimeric protein (rMELEISH) was constructed based on amino acid sequences from kinesin 39 (k39), alpha-tubulin, and heat-shock proteins HSP70 and HSP 83.1, and tested in enzyme-linked immunosorbent (ELISA) for the detection of <i>L. infantum</i> infection using canine (<i>n</i> = 140) and human (<i>n</i> = 145) sera samples. Results: in the trials, rMELEISH was able to discriminate between VL cases and cross-reactive diseases and healthy samples, with sensitivity and specificity values of 100%, as compared to the use of a soluble Leishmania antigenic extract (SLA). Conclusions: the preliminary data suggest that rMELEISH has the potential to be tested in future studies against a larger serological panel and in field conditions for the diagnosis of canine and human VL.https://www.mdpi.com/2076-0817/12/2/302leishmaniasisrecombinant chimeric proteinserodiagnosisvisceral leishmaniasishumansdogs |
| spellingShingle | Daniel Silva Dias Juliana Martins Machado Patrícia Aparecida Fernandes Ribeiro Amanda Sanchez Machado Fernanda Fonseca Ramos Lais Moreira Nogueira Ana Alice Maia Gonçalves Luana de Sousa Ramos Isadora Braga Gandra Flaviane Silva Coutinho Michelli dos Santos Jonatas Oliveira da Silva Miguel Angel Chávez-Fumagalli Rafael Gonçalves Teixeira-Neto Ana Thereza Chaves Mariana Campos-da-Paz Amanda A. Souza Rodolfo Cordeiro Giunchetti Sonia Maria Freitas Sandra Lyon Danielle Ferreira de Magalhães-Soares Julia Angelica Gonçalves Silveira Eduardo Sergio Silva Eduardo Antonio Ferraz Coelho Alexsandro Sobreira Galdino rMELEISH: A Novel Recombinant Multiepitope-Based Protein Applied to the Serodiagnosis of Both Canine and Human Visceral Leishmaniasis Pathogens leishmaniasis recombinant chimeric protein serodiagnosis visceral leishmaniasis humans dogs |
| title | rMELEISH: A Novel Recombinant Multiepitope-Based Protein Applied to the Serodiagnosis of Both Canine and Human Visceral Leishmaniasis |
| title_full | rMELEISH: A Novel Recombinant Multiepitope-Based Protein Applied to the Serodiagnosis of Both Canine and Human Visceral Leishmaniasis |
| title_fullStr | rMELEISH: A Novel Recombinant Multiepitope-Based Protein Applied to the Serodiagnosis of Both Canine and Human Visceral Leishmaniasis |
| title_full_unstemmed | rMELEISH: A Novel Recombinant Multiepitope-Based Protein Applied to the Serodiagnosis of Both Canine and Human Visceral Leishmaniasis |
| title_short | rMELEISH: A Novel Recombinant Multiepitope-Based Protein Applied to the Serodiagnosis of Both Canine and Human Visceral Leishmaniasis |
| title_sort | rmeleish a novel recombinant multiepitope based protein applied to the serodiagnosis of both canine and human visceral leishmaniasis |
| topic | leishmaniasis recombinant chimeric protein serodiagnosis visceral leishmaniasis humans dogs |
| url | https://www.mdpi.com/2076-0817/12/2/302 |
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