SERS-Based Evaluation of the DNA Methylation Pattern Associated With Progression in Clonal Leukemogenesis of Down Syndrome
Here we show that surface-enhanced Raman scattering (SERS) analysis captures the relative hypomethylation of DNA from patients with acute leukemia associated with Down syndrome (AL-DS) compared with patients diagnosed with transient leukemia associated with Down syndrome (TL-DS), an information infe...
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Frontiers Media S.A.
2021-07-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2021.703268/full |
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| author | Vlad Moisoiu Valentina Sas Valentina Sas Andrei Stefancu Stefania D. Iancu Ancuta Jurj Sergiu Pasca Sabina Iluta Alina-Andreea Zimta Adrian B. Tigu Patric Teodorescu Cristina Turcas Cristina Blag Delia Dima Gheorghe Popa Smaranda Arghirescu Smaranda Arghirescu Sorin Man Anca Colita Anca Colita Nicolae Leopold Nicolae Leopold Ciprian Tomuleasa Ciprian Tomuleasa Ciprian Tomuleasa |
| author_facet | Vlad Moisoiu Valentina Sas Valentina Sas Andrei Stefancu Stefania D. Iancu Ancuta Jurj Sergiu Pasca Sabina Iluta Alina-Andreea Zimta Adrian B. Tigu Patric Teodorescu Cristina Turcas Cristina Blag Delia Dima Gheorghe Popa Smaranda Arghirescu Smaranda Arghirescu Sorin Man Anca Colita Anca Colita Nicolae Leopold Nicolae Leopold Ciprian Tomuleasa Ciprian Tomuleasa Ciprian Tomuleasa |
| author_sort | Vlad Moisoiu |
| collection | DOAJ |
| description | Here we show that surface-enhanced Raman scattering (SERS) analysis captures the relative hypomethylation of DNA from patients with acute leukemia associated with Down syndrome (AL-DS) compared with patients diagnosed with transient leukemia associated with Down syndrome (TL-DS), an information inferred from the area under the SERS band at 1005 cm–1 attributed to 5-methycytosine. The receiver operating characteristic (ROC) analysis of the area under the SERS band at 1005 cm–1 yielded an area under the curve (AUC) of 0.77 in differentiating between the AL-DS and TL-DS groups. In addition, we showed that DNA from patients with non-DS myeloproliferative neoplasm (non-DS-MPN) is hypomethylated compared to non-DS-AL, the area under the SERS band at 1005 cm–1 yielding an AUC of 0.78 in separating between non-DS-MPN and non-DS-AL. Overall, in this study, the area of the 1005 cm–1 DNA SERS marker band shows a stepwise decrease in DNA global methylation as cells progress from a pre-leukemia to a full-blown acute leukemia, highlighting thus the potential of SERS as an emerging method of analyzing the methylation landscape of DNA in the context of leukemia genesis and progression. |
| first_indexed | 2024-12-17T07:23:09Z |
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| id | doaj.art-022013ee427e44ee9a23ad0c7a0b27bb |
| institution | Directory Open Access Journal |
| issn | 2296-4185 |
| language | English |
| last_indexed | 2024-12-17T07:23:09Z |
| publishDate | 2021-07-01 |
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| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj.art-022013ee427e44ee9a23ad0c7a0b27bb2022-12-21T21:58:41ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852021-07-01910.3389/fbioe.2021.703268703268SERS-Based Evaluation of the DNA Methylation Pattern Associated With Progression in Clonal Leukemogenesis of Down SyndromeVlad Moisoiu0Valentina Sas1Valentina Sas2Andrei Stefancu3Stefania D. Iancu4Ancuta Jurj5Sergiu Pasca6Sabina Iluta7Alina-Andreea Zimta8Adrian B. Tigu9Patric Teodorescu10Cristina Turcas11Cristina Blag12Delia Dima13Gheorghe Popa14Smaranda Arghirescu15Smaranda Arghirescu16Sorin Man17Anca Colita18Anca Colita19Nicolae Leopold20Nicolae Leopold21Ciprian Tomuleasa22Ciprian Tomuleasa23Ciprian Tomuleasa24Faculty of Physics, Babeş-Bolyai University, Cluj-Napoca, RomaniaDepartment of Hematology, Iuliu Hatieganu University of Medicine and Pharmacy, Cluj-Napoca, RomaniaDepartment of Pediatrics, Iuliu Hatieganu University of Medicine and Pharmacy, Cluj-Napoca, RomaniaFaculty of Physics, Babeş-Bolyai University, Cluj-Napoca, RomaniaFaculty of Physics, Babeş-Bolyai University, Cluj-Napoca, RomaniaResearch Center for Functional Genomics and Translational Medicine, Iuliu Hatieganu University of Medicine and Pharmacy, Cluj-Napoca, RomaniaDepartment of Hematology, Iuliu Hatieganu University of Medicine and Pharmacy, Cluj-Napoca, RomaniaDepartment of Hematology, Iuliu Hatieganu University of Medicine and Pharmacy, Cluj-Napoca, RomaniaMedfuture Research Center for Advanced Medicine, Iuliu Hatieganu University of Medicine and Pharmacy, Cluj-Napoca, RomaniaMedfuture Research Center for Advanced Medicine, Iuliu Hatieganu University of Medicine and Pharmacy, Cluj-Napoca, RomaniaDepartment of Hematology, Iuliu Hatieganu University of Medicine and Pharmacy, Cluj-Napoca, RomaniaDepartment of Hematology, Iuliu Hatieganu University of Medicine and Pharmacy, Cluj-Napoca, RomaniaDepartment of Hematology, Iuliu Hatieganu University of Medicine and Pharmacy, Cluj-Napoca, RomaniaDepartment of Hematology, Iuliu Hatieganu University of Medicine and Pharmacy, Cluj-Napoca, RomaniaDepartment of Pediatrics, Iuliu Hatieganu University of Medicine and Pharmacy, Cluj-Napoca, RomaniaDepartment of Pediatrics, Victor Babeş University of Medicine and Pharmacy, Timisoara, RomaniaDepartment of Pediatrics, Louis Turcanu Emergency Hospital for Children, Timisoara, RomaniaDepartment of Pediatrics, Iuliu Hatieganu University of Medicine and Pharmacy, Cluj-Napoca, RomaniaDepartment of Pediatrics, Carol Davila University of Medicine and Pharmacy, Bucharest, RomaniaDepartment of Pediatrics, Fundeni Clinical Institute, Bucharest, RomaniaFaculty of Physics, Babeş-Bolyai University, Cluj-Napoca, Romania0Biomed Data Analytics SRL, Cluj-Napoca, RomaniaDepartment of Hematology, Iuliu Hatieganu University of Medicine and Pharmacy, Cluj-Napoca, RomaniaMedfuture Research Center for Advanced Medicine, Iuliu Hatieganu University of Medicine and Pharmacy, Cluj-Napoca, Romania1Department of Hematology, Ion Chiricuta Clinical Cancer Center, Cluj-Napoca, RomaniaHere we show that surface-enhanced Raman scattering (SERS) analysis captures the relative hypomethylation of DNA from patients with acute leukemia associated with Down syndrome (AL-DS) compared with patients diagnosed with transient leukemia associated with Down syndrome (TL-DS), an information inferred from the area under the SERS band at 1005 cm–1 attributed to 5-methycytosine. The receiver operating characteristic (ROC) analysis of the area under the SERS band at 1005 cm–1 yielded an area under the curve (AUC) of 0.77 in differentiating between the AL-DS and TL-DS groups. In addition, we showed that DNA from patients with non-DS myeloproliferative neoplasm (non-DS-MPN) is hypomethylated compared to non-DS-AL, the area under the SERS band at 1005 cm–1 yielding an AUC of 0.78 in separating between non-DS-MPN and non-DS-AL. Overall, in this study, the area of the 1005 cm–1 DNA SERS marker band shows a stepwise decrease in DNA global methylation as cells progress from a pre-leukemia to a full-blown acute leukemia, highlighting thus the potential of SERS as an emerging method of analyzing the methylation landscape of DNA in the context of leukemia genesis and progression.https://www.frontiersin.org/articles/10.3389/fbioe.2021.703268/fullSERSdown syndromeacute leukemiatransient leukemia associated with down syndromemyeloproliferative neoplasm |
| spellingShingle | Vlad Moisoiu Valentina Sas Valentina Sas Andrei Stefancu Stefania D. Iancu Ancuta Jurj Sergiu Pasca Sabina Iluta Alina-Andreea Zimta Adrian B. Tigu Patric Teodorescu Cristina Turcas Cristina Blag Delia Dima Gheorghe Popa Smaranda Arghirescu Smaranda Arghirescu Sorin Man Anca Colita Anca Colita Nicolae Leopold Nicolae Leopold Ciprian Tomuleasa Ciprian Tomuleasa Ciprian Tomuleasa SERS-Based Evaluation of the DNA Methylation Pattern Associated With Progression in Clonal Leukemogenesis of Down Syndrome Frontiers in Bioengineering and Biotechnology SERS down syndrome acute leukemia transient leukemia associated with down syndrome myeloproliferative neoplasm |
| title | SERS-Based Evaluation of the DNA Methylation Pattern Associated With Progression in Clonal Leukemogenesis of Down Syndrome |
| title_full | SERS-Based Evaluation of the DNA Methylation Pattern Associated With Progression in Clonal Leukemogenesis of Down Syndrome |
| title_fullStr | SERS-Based Evaluation of the DNA Methylation Pattern Associated With Progression in Clonal Leukemogenesis of Down Syndrome |
| title_full_unstemmed | SERS-Based Evaluation of the DNA Methylation Pattern Associated With Progression in Clonal Leukemogenesis of Down Syndrome |
| title_short | SERS-Based Evaluation of the DNA Methylation Pattern Associated With Progression in Clonal Leukemogenesis of Down Syndrome |
| title_sort | sers based evaluation of the dna methylation pattern associated with progression in clonal leukemogenesis of down syndrome |
| topic | SERS down syndrome acute leukemia transient leukemia associated with down syndrome myeloproliferative neoplasm |
| url | https://www.frontiersin.org/articles/10.3389/fbioe.2021.703268/full |
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