Magnetic Immunosensor Coupled to Enzymatic Signal for Determination of Genomic DNA Methylation
Aberrations of genomic DNA methylation have been confirmed to be involved in the evolution of human cancer and have thus gained the potential to be depicted as biomarkers for cancer diagnostics and prognostic predictions, which implicates an urgent need for detection of total genomic DNA methylation...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-03-01
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| Series: | Biosensors |
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| Online Access: | https://www.mdpi.com/2079-6374/12/3/162 |
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| author | Yitao Liang Bin Zhang Zexin Xue Xuesong Ye Bo Liang |
| author_facet | Yitao Liang Bin Zhang Zexin Xue Xuesong Ye Bo Liang |
| author_sort | Yitao Liang |
| collection | DOAJ |
| description | Aberrations of genomic DNA methylation have been confirmed to be involved in the evolution of human cancer and have thus gained the potential to be depicted as biomarkers for cancer diagnostics and prognostic predictions, which implicates an urgent need for detection of total genomic DNA methylation. In this work, we suggested an assay for the quantification of global DNA methylation, utilizing methylation specific antibody (5mC) modified magnetic beads (MBs) for immunorecognition and affinity enrichment. Subsequently, the captured DNA on the surface of MBs interacted with the glucose oxidase-conjugated DNA antibody whose catalytic reaction product was engaged in electrochemical detection of the overall level of DNA methylation on a PB-doped screen-printed electrode. With 15 pg of input DNA, which, to our best knowledge, is the lowest required amount of DNA without sodium bisulfite treatment or amplification, this test strategy was able to perceive as low as 5% methylation level within 70 min including the preparation of anti-5mC-MBs. We believe this detection technique offers a promising option to detect global DNA methylation in both academic and clinical scenarios. |
| first_indexed | 2024-03-09T20:03:28Z |
| format | Article |
| id | doaj.art-02211118510c4a09b342fa4bbf1c1e8d |
| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-03-09T20:03:28Z |
| publishDate | 2022-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj.art-02211118510c4a09b342fa4bbf1c1e8d2023-11-24T00:36:29ZengMDPI AGBiosensors2079-63742022-03-0112316210.3390/bios12030162Magnetic Immunosensor Coupled to Enzymatic Signal for Determination of Genomic DNA MethylationYitao Liang0Bin Zhang1Zexin Xue2Xuesong Ye3Bo Liang4College of Biomedical Engineering and Instrument Science, Zhejiang University, Hangzhou 310027, ChinaSir Run-Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou 310016, ChinaCollege of Biomedical Engineering and Instrument Science, Zhejiang University, Hangzhou 310027, ChinaCollege of Biomedical Engineering and Instrument Science, Zhejiang University, Hangzhou 310027, ChinaCollege of Biomedical Engineering and Instrument Science, Zhejiang University, Hangzhou 310027, ChinaAberrations of genomic DNA methylation have been confirmed to be involved in the evolution of human cancer and have thus gained the potential to be depicted as biomarkers for cancer diagnostics and prognostic predictions, which implicates an urgent need for detection of total genomic DNA methylation. In this work, we suggested an assay for the quantification of global DNA methylation, utilizing methylation specific antibody (5mC) modified magnetic beads (MBs) for immunorecognition and affinity enrichment. Subsequently, the captured DNA on the surface of MBs interacted with the glucose oxidase-conjugated DNA antibody whose catalytic reaction product was engaged in electrochemical detection of the overall level of DNA methylation on a PB-doped screen-printed electrode. With 15 pg of input DNA, which, to our best knowledge, is the lowest required amount of DNA without sodium bisulfite treatment or amplification, this test strategy was able to perceive as low as 5% methylation level within 70 min including the preparation of anti-5mC-MBs. We believe this detection technique offers a promising option to detect global DNA methylation in both academic and clinical scenarios.https://www.mdpi.com/2079-6374/12/3/162DNA methylationmagnetic beadsscreen printed electrodeelectrochemical |
| spellingShingle | Yitao Liang Bin Zhang Zexin Xue Xuesong Ye Bo Liang Magnetic Immunosensor Coupled to Enzymatic Signal for Determination of Genomic DNA Methylation Biosensors DNA methylation magnetic beads screen printed electrode electrochemical |
| title | Magnetic Immunosensor Coupled to Enzymatic Signal for Determination of Genomic DNA Methylation |
| title_full | Magnetic Immunosensor Coupled to Enzymatic Signal for Determination of Genomic DNA Methylation |
| title_fullStr | Magnetic Immunosensor Coupled to Enzymatic Signal for Determination of Genomic DNA Methylation |
| title_full_unstemmed | Magnetic Immunosensor Coupled to Enzymatic Signal for Determination of Genomic DNA Methylation |
| title_short | Magnetic Immunosensor Coupled to Enzymatic Signal for Determination of Genomic DNA Methylation |
| title_sort | magnetic immunosensor coupled to enzymatic signal for determination of genomic dna methylation |
| topic | DNA methylation magnetic beads screen printed electrode electrochemical |
| url | https://www.mdpi.com/2079-6374/12/3/162 |
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