Evaluation of aberrant expression of CD markers in acute leukemia cells
Background and objective: Worldwide immunophenotyping by flow cytometry (FCM) in acute leukemia (AL) is the golden step in the diagnosis. It’s very common for acute leukemias to aberrantly express antigens or cluster of differentiation (CD) markers which are usually expressed in other lineages of th...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Hawler Medical University
2023-08-01
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| Series: | Zanco Journal of Medical Sciences |
| Subjects: | |
| Online Access: | https://zjms.hmu.edu.krd/index.php/zjms/article/view/906 |
| _version_ | 1797737685842919424 |
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| author | Lava kareem Shwani Nawsherwan Sadiq Muhammad Hiwa Hassan Hamza |
| author_facet | Lava kareem Shwani Nawsherwan Sadiq Muhammad Hiwa Hassan Hamza |
| author_sort | Lava kareem Shwani |
| collection | DOAJ |
| description | Background and objective: Worldwide immunophenotyping by flow cytometry (FCM) in acute leukemia (AL) is the golden step in the diagnosis. It’s very common for acute leukemias to aberrantly express antigens or cluster of differentiation (CD) markers which are usually expressed in other lineages of the disease hence this study aimed at determining the prevalence of aberrancy in AL and to find out the frequency of each aberrant CD marker and their association with the clinic-hematological profile of the cases.
Methods: Following history and clinical examination of enrolled patients, blood and/or bone marrow aspirate was drawn for morphological examination and immunophenotyping by FCM from 86 newly diagnosed acute leukemia cases then multiple steps procedure was applied followed by interpretation of the results.
Results: The prevalence of aberrant phenotype was 46.5%. The proportional frequency of aberrant phenotype in acute myeloid leukemia (AML) was 41%, in B-acute lymphoblastic leukemia (B-ALL) was 48.8% and in T-acute lymphoblastic leukemia (T-ALL) was 66.6%. The commonest aberrant CD markers in AML were CD22 and CD2, in B-ALL were CD66c and CD13 while in T-ALL were CD13 and CD33. The aberrant phenotype harbored lower white blood cell (WBC) count and blast percentage in PB, also splenomegaly was more frequent in lymphoid positive (Ly+) AML and myeloid positive (My+) T-ALL while in B-ALL, splenomegaly was more frequent in myeloid negative (My-) B-ALL.
Conclusion: Aberrant phenotype prevalence in our study sample was comparable to other studies, considerable frequency of aberrant markers is present in cases of AL and some variations exist regarding the clinical and hematological profile of the aberrant group. |
| first_indexed | 2024-03-12T13:32:00Z |
| format | Article |
| id | doaj.art-0223248ba5984a63a6fcaec5c8b57666 |
| institution | Directory Open Access Journal |
| issn | 1995-5588 1995-5596 |
| language | English |
| last_indexed | 2024-03-12T13:32:00Z |
| publishDate | 2023-08-01 |
| publisher | Hawler Medical University |
| record_format | Article |
| series | Zanco Journal of Medical Sciences |
| spelling | doaj.art-0223248ba5984a63a6fcaec5c8b576662023-08-24T07:21:36ZengHawler Medical UniversityZanco Journal of Medical Sciences1995-55881995-55962023-08-0127219420410.15218/zjms.2023.022Evaluation of aberrant expression of CD markers in acute leukemia cellsLava kareem Shwani0Nawsherwan Sadiq Muhammad1Hiwa Hassan Hamza2Department of Basic Sciences, College of Medicine, Hawler Medical University, Erbil, Iraq.Department of Basic Sciences, College of Medicine, Hawler Medical University, Erbil, Iraq.Department of Laboratory, Flow Cytometry Unit, Nanakaly Hospital for Blood Diseases, Erbil, Iraq.Background and objective: Worldwide immunophenotyping by flow cytometry (FCM) in acute leukemia (AL) is the golden step in the diagnosis. It’s very common for acute leukemias to aberrantly express antigens or cluster of differentiation (CD) markers which are usually expressed in other lineages of the disease hence this study aimed at determining the prevalence of aberrancy in AL and to find out the frequency of each aberrant CD marker and their association with the clinic-hematological profile of the cases. Methods: Following history and clinical examination of enrolled patients, blood and/or bone marrow aspirate was drawn for morphological examination and immunophenotyping by FCM from 86 newly diagnosed acute leukemia cases then multiple steps procedure was applied followed by interpretation of the results. Results: The prevalence of aberrant phenotype was 46.5%. The proportional frequency of aberrant phenotype in acute myeloid leukemia (AML) was 41%, in B-acute lymphoblastic leukemia (B-ALL) was 48.8% and in T-acute lymphoblastic leukemia (T-ALL) was 66.6%. The commonest aberrant CD markers in AML were CD22 and CD2, in B-ALL were CD66c and CD13 while in T-ALL were CD13 and CD33. The aberrant phenotype harbored lower white blood cell (WBC) count and blast percentage in PB, also splenomegaly was more frequent in lymphoid positive (Ly+) AML and myeloid positive (My+) T-ALL while in B-ALL, splenomegaly was more frequent in myeloid negative (My-) B-ALL. Conclusion: Aberrant phenotype prevalence in our study sample was comparable to other studies, considerable frequency of aberrant markers is present in cases of AL and some variations exist regarding the clinical and hematological profile of the aberrant group.https://zjms.hmu.edu.krd/index.php/zjms/article/view/906aberrant phenotypeflow cytometryacute leukemiaamlb-allt-all |
| spellingShingle | Lava kareem Shwani Nawsherwan Sadiq Muhammad Hiwa Hassan Hamza Evaluation of aberrant expression of CD markers in acute leukemia cells Zanco Journal of Medical Sciences aberrant phenotype flow cytometry acute leukemia aml b-all t-all |
| title | Evaluation of aberrant expression of CD markers in acute leukemia cells |
| title_full | Evaluation of aberrant expression of CD markers in acute leukemia cells |
| title_fullStr | Evaluation of aberrant expression of CD markers in acute leukemia cells |
| title_full_unstemmed | Evaluation of aberrant expression of CD markers in acute leukemia cells |
| title_short | Evaluation of aberrant expression of CD markers in acute leukemia cells |
| title_sort | evaluation of aberrant expression of cd markers in acute leukemia cells |
| topic | aberrant phenotype flow cytometry acute leukemia aml b-all t-all |
| url | https://zjms.hmu.edu.krd/index.php/zjms/article/view/906 |
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