Effects of vasoactive substances on biomechanics of small resistance arteries of male and female Dahl salt‐sensitive rats
Abstract Changes in vascular biomechanics leading to increase in arterial stiffness play a pivotal role in circulatory dysfunction. Our objectives were to examine sex‐specific pharmacological changes related to the biomechanics and any structural modifications in small resistance arteries of Dahl sa...
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| Format: | Article |
| Language: | English |
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Wiley
2024-04-01
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| Series: | Pharmacology Research & Perspectives |
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| Online Access: | https://doi.org/10.1002/prp2.1180 |
| _version_ | 1797213291001413632 |
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| author | Eric A. Mensah Noriko Daneshtalab Reza Tabrizchi |
| author_facet | Eric A. Mensah Noriko Daneshtalab Reza Tabrizchi |
| author_sort | Eric A. Mensah |
| collection | DOAJ |
| description | Abstract Changes in vascular biomechanics leading to increase in arterial stiffness play a pivotal role in circulatory dysfunction. Our objectives were to examine sex‐specific pharmacological changes related to the biomechanics and any structural modifications in small resistance arteries of Dahl salt‐sensitive male and female rats. The composite Young modulus (CYM) was determined using pressure myograph recordings, and immunohistochemistry was used for the evaluation of any structural changes in the third‐order mesenteric arteries (n = 6). Animals on high‐salt diet developed hypertension with significant elevation in central and peripheral blood pressures and pulse wave velocity compared to those on regular diet. There were no significant differences observed in the CYM between any of the groups (i.e., males and females) in vehicle‐treated time‐control studies. The presence of verapamil (0.3 μM) significantly reduced CYM in hypertensive males without changes within females compared to vehicle. This effect was abolished by phenylephrine (0.3 μM). BaCl2 (100 μM), ouabain (100 μM), and L‐NAME (0.3 μM) combined significantly increased CYM in vessels from in normotensive males and females but not in hypertensive males compared to vehicle. The increase in CYM was abolished in the presence of phenylephrine. Sodium nitroprusside (0.3 μM), in the presence of phenylephrine, significantly reduced CYM in male normotensive versus hypertensive, with no differences within females. Significant differences were observed in immunohistochemical assessment of biomechanical markers of arterial stiffness between males and females. Our findings suggest sex possibly due to pressure differences to be responsible for adaptive changes in biomechanics, and varied pharmacological responses in hypertensive state. |
| first_indexed | 2024-04-24T10:55:56Z |
| format | Article |
| id | doaj.art-02237ac4eafa4cb692641c203dc6bb20 |
| institution | Directory Open Access Journal |
| issn | 2052-1707 |
| language | English |
| last_indexed | 2024-04-24T10:55:56Z |
| publishDate | 2024-04-01 |
| publisher | Wiley |
| record_format | Article |
| series | Pharmacology Research & Perspectives |
| spelling | doaj.art-02237ac4eafa4cb692641c203dc6bb202024-04-12T05:54:30ZengWileyPharmacology Research & Perspectives2052-17072024-04-01122n/an/a10.1002/prp2.1180Effects of vasoactive substances on biomechanics of small resistance arteries of male and female Dahl salt‐sensitive ratsEric A. Mensah0Noriko Daneshtalab1Reza Tabrizchi2Division of BioMedical Sciences, Faculty of Medicine Memorial University of Newfoundland St. John's Newfoundland CanadaSchool of Pharmacy Memorial University Newfoundland St. John's Newfoundland CanadaDivision of BioMedical Sciences, Faculty of Medicine Memorial University of Newfoundland St. John's Newfoundland CanadaAbstract Changes in vascular biomechanics leading to increase in arterial stiffness play a pivotal role in circulatory dysfunction. Our objectives were to examine sex‐specific pharmacological changes related to the biomechanics and any structural modifications in small resistance arteries of Dahl salt‐sensitive male and female rats. The composite Young modulus (CYM) was determined using pressure myograph recordings, and immunohistochemistry was used for the evaluation of any structural changes in the third‐order mesenteric arteries (n = 6). Animals on high‐salt diet developed hypertension with significant elevation in central and peripheral blood pressures and pulse wave velocity compared to those on regular diet. There were no significant differences observed in the CYM between any of the groups (i.e., males and females) in vehicle‐treated time‐control studies. The presence of verapamil (0.3 μM) significantly reduced CYM in hypertensive males without changes within females compared to vehicle. This effect was abolished by phenylephrine (0.3 μM). BaCl2 (100 μM), ouabain (100 μM), and L‐NAME (0.3 μM) combined significantly increased CYM in vessels from in normotensive males and females but not in hypertensive males compared to vehicle. The increase in CYM was abolished in the presence of phenylephrine. Sodium nitroprusside (0.3 μM), in the presence of phenylephrine, significantly reduced CYM in male normotensive versus hypertensive, with no differences within females. Significant differences were observed in immunohistochemical assessment of biomechanical markers of arterial stiffness between males and females. Our findings suggest sex possibly due to pressure differences to be responsible for adaptive changes in biomechanics, and varied pharmacological responses in hypertensive state.https://doi.org/10.1002/prp2.1180salt‐induced hypertensionsex differencesvascular function and structurevasoactive agents |
| spellingShingle | Eric A. Mensah Noriko Daneshtalab Reza Tabrizchi Effects of vasoactive substances on biomechanics of small resistance arteries of male and female Dahl salt‐sensitive rats Pharmacology Research & Perspectives salt‐induced hypertension sex differences vascular function and structure vasoactive agents |
| title | Effects of vasoactive substances on biomechanics of small resistance arteries of male and female Dahl salt‐sensitive rats |
| title_full | Effects of vasoactive substances on biomechanics of small resistance arteries of male and female Dahl salt‐sensitive rats |
| title_fullStr | Effects of vasoactive substances on biomechanics of small resistance arteries of male and female Dahl salt‐sensitive rats |
| title_full_unstemmed | Effects of vasoactive substances on biomechanics of small resistance arteries of male and female Dahl salt‐sensitive rats |
| title_short | Effects of vasoactive substances on biomechanics of small resistance arteries of male and female Dahl salt‐sensitive rats |
| title_sort | effects of vasoactive substances on biomechanics of small resistance arteries of male and female dahl salt sensitive rats |
| topic | salt‐induced hypertension sex differences vascular function and structure vasoactive agents |
| url | https://doi.org/10.1002/prp2.1180 |
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