Circadian behavior of mice deficient in PER1/PML or PER2/PML
Background: Our recent studies demonstrate that the murine homolog of the human tumor suppressor promyelocytic leukemia (PML) regulates circadian behavior of mice. To further gather insight into PML’s contribution to circadian behavior, we generated two strains of mice deficient in one of the two pe...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Ubiquity Press
2013-08-01
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| Series: | Journal of Circadian Rhythms |
| Subjects: | |
| Online Access: | https://www.jcircadianrhythms.com/articles/5 |
| _version_ | 1819055314170806272 |
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| author | Takao Miki Misty Chen-Goodspeed Zhaoyang Zhao Cheng Chi Lee |
| author_facet | Takao Miki Misty Chen-Goodspeed Zhaoyang Zhao Cheng Chi Lee |
| author_sort | Takao Miki |
| collection | DOAJ |
| description | Background: Our recent studies demonstrate that the murine homolog of the human tumor suppressor promyelocytic leukemia (PML) regulates circadian behavior of mice. To further gather insight into PML’s contribution to circadian behavior, we generated two strains of mice deficient in one of the two period ('Per') genes and the PML gene, with 'Per1''−/−''/Pml''−/−' and 'Per2''−/−''/Pml''−/−' genotypes. Results: Here we report the circadian behavior of these mice based on wheel-running behavioral analysis. In a free-running environment, the 'Per1''−/−''/Pml''−/−' mice maintained circadian rhythm but displayed a significantly shorter period of 22.2 h. In addition, these mice displayed significantly enhanced phase response to a light pulse given at zeitgeber time (ZT) 14 and 22. The 'Per2''−/−''/Pml''−/−' mice lose persistent rhythm when in a free-running environment, as also the case for'Per2'−/− mice'.' A transient post-light pulse rhythm seen in the arrhythmic 'Per2''−/−' mice was less apparent in 'Per2''−/−''/Pml''−/−' mice. Both the 'Per1''−/−''/Pml''−/−' and 'Per2''−/−''/Pml''−/−' mice displayed a more advanced phase angle of entrainment activity during light–dark cycles than the single gene deficient mice. Conclusions: Beyond merely regulating PER1 and PER2, the current behavioral studies suggest PML has additional roles in mouse circadian behavior. |
| first_indexed | 2024-12-21T13:05:33Z |
| format | Article |
| id | doaj.art-0223903c1c7e437a87c084a159d3a913 |
| institution | Directory Open Access Journal |
| issn | 1740-3391 |
| language | English |
| last_indexed | 2024-12-21T13:05:33Z |
| publishDate | 2013-08-01 |
| publisher | Ubiquity Press |
| record_format | Article |
| series | Journal of Circadian Rhythms |
| spelling | doaj.art-0223903c1c7e437a87c084a159d3a9132022-12-21T19:03:01ZengUbiquity PressJournal of Circadian Rhythms1740-33912013-08-011110.1186/1740-3391-11-95Circadian behavior of mice deficient in PER1/PML or PER2/PMLTakao Miki0Misty Chen-Goodspeed1Zhaoyang Zhao2Cheng Chi Lee3Department of Biochemistry and Molecular Biology, Medical School, University of Texas Health Science Center-Houston, Houston, TX and Department of Molecular Oncology, Kyoto University Graduate School of Medicine, KyotoDepartment of Biochemistry and Molecular Biology, Medical School, University of Texas Health Science Center-Houston, Houston, TXDepartment of Biochemistry and Molecular Biology, Medical School, University of Texas Health Science Center-Houston, Houston, TXDepartment of Biochemistry and Molecular Biology, Medical School, University of Texas Health Science Center-Houston, Houston, TXBackground: Our recent studies demonstrate that the murine homolog of the human tumor suppressor promyelocytic leukemia (PML) regulates circadian behavior of mice. To further gather insight into PML’s contribution to circadian behavior, we generated two strains of mice deficient in one of the two period ('Per') genes and the PML gene, with 'Per1''−/−''/Pml''−/−' and 'Per2''−/−''/Pml''−/−' genotypes. Results: Here we report the circadian behavior of these mice based on wheel-running behavioral analysis. In a free-running environment, the 'Per1''−/−''/Pml''−/−' mice maintained circadian rhythm but displayed a significantly shorter period of 22.2 h. In addition, these mice displayed significantly enhanced phase response to a light pulse given at zeitgeber time (ZT) 14 and 22. The 'Per2''−/−''/Pml''−/−' mice lose persistent rhythm when in a free-running environment, as also the case for'Per2'−/− mice'.' A transient post-light pulse rhythm seen in the arrhythmic 'Per2''−/−' mice was less apparent in 'Per2''−/−''/Pml''−/−' mice. Both the 'Per1''−/−''/Pml''−/−' and 'Per2''−/−''/Pml''−/−' mice displayed a more advanced phase angle of entrainment activity during light–dark cycles than the single gene deficient mice. Conclusions: Beyond merely regulating PER1 and PER2, the current behavioral studies suggest PML has additional roles in mouse circadian behavior.https://www.jcircadianrhythms.com/articles/5Circadian rhythmMouse modelActogramPhase shiftPeriod |
| spellingShingle | Takao Miki Misty Chen-Goodspeed Zhaoyang Zhao Cheng Chi Lee Circadian behavior of mice deficient in PER1/PML or PER2/PML Journal of Circadian Rhythms Circadian rhythm Mouse model Actogram Phase shift Period |
| title | Circadian behavior of mice deficient in PER1/PML or PER2/PML |
| title_full | Circadian behavior of mice deficient in PER1/PML or PER2/PML |
| title_fullStr | Circadian behavior of mice deficient in PER1/PML or PER2/PML |
| title_full_unstemmed | Circadian behavior of mice deficient in PER1/PML or PER2/PML |
| title_short | Circadian behavior of mice deficient in PER1/PML or PER2/PML |
| title_sort | circadian behavior of mice deficient in per1 pml or per2 pml |
| topic | Circadian rhythm Mouse model Actogram Phase shift Period |
| url | https://www.jcircadianrhythms.com/articles/5 |
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