In vitro assessment of the anthelmintic activity of copper oxide and zinc oxide nanoparticles on egg and adult stages of Fasciola hepatica: evidence on oxidative stress biomarkers, and DNA damage
Abstract Objectives Fasciolosis is of significant economic and public health importance worldwide. The lack of a successful vaccine and emerging resistance in flukes to the drug of choice, triclabendazole, has initiated the search for alternative approaches. In recent years, metallic nanoparticles h...
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| Format: | Article |
| Language: | English |
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BMC
2024-04-01
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| Series: | BMC Veterinary Research |
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| Online Access: | https://doi.org/10.1186/s12917-024-03994-0 |
| _version_ | 1797219707138342912 |
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| author | Amirhassan Ravvaz Farnaz Malekifard Bijan Esmaeilnejad |
| author_facet | Amirhassan Ravvaz Farnaz Malekifard Bijan Esmaeilnejad |
| author_sort | Amirhassan Ravvaz |
| collection | DOAJ |
| description | Abstract Objectives Fasciolosis is of significant economic and public health importance worldwide. The lack of a successful vaccine and emerging resistance in flukes to the drug of choice, triclabendazole, has initiated the search for alternative approaches. In recent years, metallic nanoparticles have been extensively investigated for their anthelmintic effects. This study investigates the in vitro anthelmintic activity of copper oxide and zinc oxide nanoparticles against Fasciola hepatica. Methods The in vitro study was based on egg hatchability test (EHA), adult motility inhibition tests, DNA damage, ROS levels, as well as several biomarkers of oxidative stress, including glutathione peroxidase (GSH) and glutathione S-transferase (GST), superoxide dismutase (SOD) and malondialdehyde (MDA). For this purpose, different concentrations of copper oxide nanoparticles (CuO-NPs) and Zinc oxide nanoparticles (ZnO-NPs) (1, 4, 8, 12, and 16 ppm) were used to evaluate the anthelmintic effect on different life stages, including egg and adults of Fasciola hepatica, over 24 h. Results In vitro treatment of F. hepatica worms with both CuO-NPs and ZnO-NPs could significantly increase ROS production and oxidative stress induction (decreased SOD, GST and GSH and increased MDA) compared to control group. Conclusions Based on the results, it seems that CuO-NPs and ZnO-NPs may be effective in the control and treatment of F. hepatica infection. Further research is needed to investigate their potential for in vivo use in the treatment of parasitic infections. |
| first_indexed | 2024-04-24T12:37:55Z |
| format | Article |
| id | doaj.art-0223e0ccf561451997abc626973e440f |
| institution | Directory Open Access Journal |
| issn | 1746-6148 |
| language | English |
| last_indexed | 2024-04-24T12:37:55Z |
| publishDate | 2024-04-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Veterinary Research |
| spelling | doaj.art-0223e0ccf561451997abc626973e440f2024-04-07T11:21:42ZengBMCBMC Veterinary Research1746-61482024-04-0120111010.1186/s12917-024-03994-0In vitro assessment of the anthelmintic activity of copper oxide and zinc oxide nanoparticles on egg and adult stages of Fasciola hepatica: evidence on oxidative stress biomarkers, and DNA damageAmirhassan Ravvaz0Farnaz Malekifard1Bijan Esmaeilnejad2Department of Pathobiology, Faculty of Veterinary Medicine, Urmia UniversityDepartment of Pathobiology, Faculty of Veterinary Medicine, Urmia UniversityDepartment of Pathobiology, Faculty of Veterinary Medicine, Urmia UniversityAbstract Objectives Fasciolosis is of significant economic and public health importance worldwide. The lack of a successful vaccine and emerging resistance in flukes to the drug of choice, triclabendazole, has initiated the search for alternative approaches. In recent years, metallic nanoparticles have been extensively investigated for their anthelmintic effects. This study investigates the in vitro anthelmintic activity of copper oxide and zinc oxide nanoparticles against Fasciola hepatica. Methods The in vitro study was based on egg hatchability test (EHA), adult motility inhibition tests, DNA damage, ROS levels, as well as several biomarkers of oxidative stress, including glutathione peroxidase (GSH) and glutathione S-transferase (GST), superoxide dismutase (SOD) and malondialdehyde (MDA). For this purpose, different concentrations of copper oxide nanoparticles (CuO-NPs) and Zinc oxide nanoparticles (ZnO-NPs) (1, 4, 8, 12, and 16 ppm) were used to evaluate the anthelmintic effect on different life stages, including egg and adults of Fasciola hepatica, over 24 h. Results In vitro treatment of F. hepatica worms with both CuO-NPs and ZnO-NPs could significantly increase ROS production and oxidative stress induction (decreased SOD, GST and GSH and increased MDA) compared to control group. Conclusions Based on the results, it seems that CuO-NPs and ZnO-NPs may be effective in the control and treatment of F. hepatica infection. Further research is needed to investigate their potential for in vivo use in the treatment of parasitic infections.https://doi.org/10.1186/s12917-024-03994-0Copper oxide nanoparticlesDNA damageFasciola hepaticaIn vitroAnthelmintic activityOxidative stress |
| spellingShingle | Amirhassan Ravvaz Farnaz Malekifard Bijan Esmaeilnejad In vitro assessment of the anthelmintic activity of copper oxide and zinc oxide nanoparticles on egg and adult stages of Fasciola hepatica: evidence on oxidative stress biomarkers, and DNA damage BMC Veterinary Research Copper oxide nanoparticles DNA damage Fasciola hepatica In vitro Anthelmintic activity Oxidative stress |
| title | In vitro assessment of the anthelmintic activity of copper oxide and zinc oxide nanoparticles on egg and adult stages of Fasciola hepatica: evidence on oxidative stress biomarkers, and DNA damage |
| title_full | In vitro assessment of the anthelmintic activity of copper oxide and zinc oxide nanoparticles on egg and adult stages of Fasciola hepatica: evidence on oxidative stress biomarkers, and DNA damage |
| title_fullStr | In vitro assessment of the anthelmintic activity of copper oxide and zinc oxide nanoparticles on egg and adult stages of Fasciola hepatica: evidence on oxidative stress biomarkers, and DNA damage |
| title_full_unstemmed | In vitro assessment of the anthelmintic activity of copper oxide and zinc oxide nanoparticles on egg and adult stages of Fasciola hepatica: evidence on oxidative stress biomarkers, and DNA damage |
| title_short | In vitro assessment of the anthelmintic activity of copper oxide and zinc oxide nanoparticles on egg and adult stages of Fasciola hepatica: evidence on oxidative stress biomarkers, and DNA damage |
| title_sort | in vitro assessment of the anthelmintic activity of copper oxide and zinc oxide nanoparticles on egg and adult stages of fasciola hepatica evidence on oxidative stress biomarkers and dna damage |
| topic | Copper oxide nanoparticles DNA damage Fasciola hepatica In vitro Anthelmintic activity Oxidative stress |
| url | https://doi.org/10.1186/s12917-024-03994-0 |
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