Loop-mediated isothermal amplification for diagnosing marine pathogens in tissues of Crassostrea spp. and white shrimp, Litopenaeus vannamei, farmed in Mexico
Loop-mediated isothermal amplification (LAMP) is an accurate, sensitive, rapid, and easy-to-perform method for gene amplification under isothermal conditions, and it has served as a powerful diagnostic tool. In this study, we used LAMP to develop a diagnostic protocol for detecting Vibrio parahaemo...
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| Format: | Article |
| Language: | English |
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Universidad Autónoma de Baja California
2021-12-01
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| Series: | Ciencias Marinas |
| Subjects: | |
| Online Access: | https://www.cienciasmarinas.com.mx/index.php/cmarinas/article/view/3095 |
| _version_ | 1797279533359955968 |
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| author | Ismael Mendoza-Avilés Carla A Muñoz-Rojas Mario Rojas Norma Estrada |
| author_facet | Ismael Mendoza-Avilés Carla A Muñoz-Rojas Mario Rojas Norma Estrada |
| author_sort | Ismael Mendoza-Avilés |
| collection | DOAJ |
| description |
Loop-mediated isothermal amplification (LAMP) is an accurate, sensitive, rapid, and easy-to-perform method for gene amplification under isothermal conditions, and it has served as a powerful diagnostic tool. In this study, we used LAMP to develop a diagnostic protocol for detecting Vibrio parahaemolyticus and white spot syndrome virus in whiteleg shrimp, and Perkinsus spp. in Crassostrea spp. in Mexico. These pathogens are associated with different diseases and are considered a threat in the aquaculture industry. Infected and uninfected oysters and shrimp were obtained from farms in the northwest coast of Mexico to standardize the LAMP assay. We determined the candidate target genes in the first-round analysis of many sets of primers, and then we chose a set of primers that successfully amplified with Mexican samples. We optimized the LAMP reactions for each pathogen with the chosen primer sets using temperature gradients from 61 to 65 ºC, DNA concentrations from 2.5 pg to 250.0 ng, and reaction times from 10 to 60 min. This study established a diagnostic procedure for detecting pathogens in oysters and shrimp from Mexico. Early diagnosis and treatment of pathogens can immensely reduce disease transmission in aquaculture farms.
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| first_indexed | 2024-03-07T16:26:14Z |
| format | Article |
| id | doaj.art-0225c5ff70b947abb3bbc4f9e93bc7a9 |
| institution | Directory Open Access Journal |
| issn | 0185-3880 2395-9053 |
| language | English |
| last_indexed | 2024-03-07T16:26:14Z |
| publishDate | 2021-12-01 |
| publisher | Universidad Autónoma de Baja California |
| record_format | Article |
| series | Ciencias Marinas |
| spelling | doaj.art-0225c5ff70b947abb3bbc4f9e93bc7a92024-03-03T17:31:34ZengUniversidad Autónoma de Baja CaliforniaCiencias Marinas0185-38802395-90532021-12-0147410.7773/cm.v47i4.3095Loop-mediated isothermal amplification for diagnosing marine pathogens in tissues of Crassostrea spp. and white shrimp, Litopenaeus vannamei, farmed in MexicoIsmael Mendoza-Avilés0Carla A Muñoz-Rojas1Mario Rojas2Norma Estrada3Universidad Autónoma de Baja California SurCentro de Investigaciones Biológicas del NoroesteCentro de Investigaciones Biológicas del NoroesteCentro de Investigaciones Biológicas del Noroeste Loop-mediated isothermal amplification (LAMP) is an accurate, sensitive, rapid, and easy-to-perform method for gene amplification under isothermal conditions, and it has served as a powerful diagnostic tool. In this study, we used LAMP to develop a diagnostic protocol for detecting Vibrio parahaemolyticus and white spot syndrome virus in whiteleg shrimp, and Perkinsus spp. in Crassostrea spp. in Mexico. These pathogens are associated with different diseases and are considered a threat in the aquaculture industry. Infected and uninfected oysters and shrimp were obtained from farms in the northwest coast of Mexico to standardize the LAMP assay. We determined the candidate target genes in the first-round analysis of many sets of primers, and then we chose a set of primers that successfully amplified with Mexican samples. We optimized the LAMP reactions for each pathogen with the chosen primer sets using temperature gradients from 61 to 65 ºC, DNA concentrations from 2.5 pg to 250.0 ng, and reaction times from 10 to 60 min. This study established a diagnostic procedure for detecting pathogens in oysters and shrimp from Mexico. Early diagnosis and treatment of pathogens can immensely reduce disease transmission in aquaculture farms. https://www.cienciasmarinas.com.mx/index.php/cmarinas/article/view/3095Crassostrea spp.Litopenaeus vannameiloop-mediated isothermal amplificationaquaculturepathogens |
| spellingShingle | Ismael Mendoza-Avilés Carla A Muñoz-Rojas Mario Rojas Norma Estrada Loop-mediated isothermal amplification for diagnosing marine pathogens in tissues of Crassostrea spp. and white shrimp, Litopenaeus vannamei, farmed in Mexico Ciencias Marinas Crassostrea spp. Litopenaeus vannamei loop-mediated isothermal amplification aquaculture pathogens |
| title | Loop-mediated isothermal amplification for diagnosing marine pathogens in tissues of Crassostrea spp. and white shrimp, Litopenaeus vannamei, farmed in Mexico |
| title_full | Loop-mediated isothermal amplification for diagnosing marine pathogens in tissues of Crassostrea spp. and white shrimp, Litopenaeus vannamei, farmed in Mexico |
| title_fullStr | Loop-mediated isothermal amplification for diagnosing marine pathogens in tissues of Crassostrea spp. and white shrimp, Litopenaeus vannamei, farmed in Mexico |
| title_full_unstemmed | Loop-mediated isothermal amplification for diagnosing marine pathogens in tissues of Crassostrea spp. and white shrimp, Litopenaeus vannamei, farmed in Mexico |
| title_short | Loop-mediated isothermal amplification for diagnosing marine pathogens in tissues of Crassostrea spp. and white shrimp, Litopenaeus vannamei, farmed in Mexico |
| title_sort | loop mediated isothermal amplification for diagnosing marine pathogens in tissues of crassostrea spp and white shrimp litopenaeus vannamei farmed in mexico |
| topic | Crassostrea spp. Litopenaeus vannamei loop-mediated isothermal amplification aquaculture pathogens |
| url | https://www.cienciasmarinas.com.mx/index.php/cmarinas/article/view/3095 |
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