Molecular typing and drug sensitivity profiles of M. Tuberculosis isolated from refugees residing in Ethiopia
Background: Refugees in developing countries have poor access to Tuberculosis (TB) care and control services. The understanding of genetic diversity and drug sensitivity patterns of M. tuberculosis (MTB) is important for the TB control program. However, there is no evidence that shows the drug sensi...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-05-01
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| Series: | Journal of Clinical Tuberculosis and Other Mycobacterial Diseases |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S240557942300027X |
| _version_ | 1797837747986104320 |
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| author | Abyot Meaza Getu Diriba Musse Girma Ammanuel Wondimu Getnet Worku Girmay Medhin Gobena Ameni Balako Gumi |
| author_facet | Abyot Meaza Getu Diriba Musse Girma Ammanuel Wondimu Getnet Worku Girmay Medhin Gobena Ameni Balako Gumi |
| author_sort | Abyot Meaza |
| collection | DOAJ |
| description | Background: Refugees in developing countries have poor access to Tuberculosis (TB) care and control services. The understanding of genetic diversity and drug sensitivity patterns of M. tuberculosis (MTB) is important for the TB control program. However, there is no evidence that shows the drug sensitivity profiles and genetic diversity of MTB circulating among refugees residing in Ethiopia. This study aimed to investigate the genetic diversity of MTB strains and lineages, and to identify the drug sensitivity profiles of MTB isolated from refugees residing in Ethiopia. Methods: A cross-sectional study was conducted among 68 MTB positive cases isolated from presumptive TB refugees from February to August 2021. Data and samples were collected in the refugee camp clinics and both rapid TB Ag detection and region of difference (RD)-9 deletion typing were used to confirm the MTBs. Drug susceptibility test (DST) and molecular typing were done using Mycobacterium Growth Indicator Tube (MGIT) method and spoligotyping respectively. Results: DST and spoligotyping results were available for all 68 isolates. The isolates were grouped into 25 spoligotype patterns, which consisted of 1–31 isolates with 36.8% strain diversity. The international shared type (SIT)25 was predominant spoligotype pattern consisting of 31 (45.6%) isolates, followed by SIT24 comprising 5 (7.4%) isolates. Further investigation showed that 64.7% (44/68) of the isolates were belonged to CAS1-Delhi family and 75% (51/68) of the isolates were belonged to lineage(L)-3. Multi-drug resistance (MDR)-TB was observed only in one isolate (1.5%) for first-line anti-TB drugs and the highest level of mono-resistance, 5.9% (4/68), was observed for PZA(Pyrazinamide). Mono-resistance was observed in 2.9 % (2/68) and while 97.0% (66/68) of the MTB positive cases were susceptible to the second-line anti-TB drugs. Conclusion: The findings are useful evidence for the TB screening, treatment and control in refugee populations and surrounding communities in Ethiopia. |
| first_indexed | 2024-04-09T15:29:40Z |
| format | Article |
| id | doaj.art-0227ad78be994b76b9059541c5fdbb17 |
| institution | Directory Open Access Journal |
| issn | 2405-5794 |
| language | English |
| last_indexed | 2024-04-09T15:29:40Z |
| publishDate | 2023-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Clinical Tuberculosis and Other Mycobacterial Diseases |
| spelling | doaj.art-0227ad78be994b76b9059541c5fdbb172023-04-28T08:55:57ZengElsevierJournal of Clinical Tuberculosis and Other Mycobacterial Diseases2405-57942023-05-0131100371Molecular typing and drug sensitivity profiles of M. Tuberculosis isolated from refugees residing in EthiopiaAbyot Meaza0Getu Diriba1Musse Girma2Ammanuel Wondimu3Getnet Worku4Girmay Medhin5Gobena Ameni6Balako Gumi7Aklilu Lemma Institute of Pathobiology (ALIPB), Addis Ababa University (AAU), P.O. Box 1176, Addis Ababa, Ethiopia; Ethiopian Public Health Institute (EPHI), PO Box 1242, Swaziland Street, Addis Ababa, Ethiopia; Corresponding author.Ethiopian Public Health Institute (EPHI), PO Box 1242, Swaziland Street, Addis Ababa, EthiopiaAklilu Lemma Institute of Pathobiology (ALIPB), Addis Ababa University (AAU), P.O. Box 1176, Addis Ababa, EthiopiaEthiopian Public Health Institute (EPHI), PO Box 1242, Swaziland Street, Addis Ababa, EthiopiaAklilu Lemma Institute of Pathobiology (ALIPB), Addis Ababa University (AAU), P.O. Box 1176, Addis Ababa, Ethiopia; Department of Medical Laboratory Science, College of Medicine and Health Sciences, Jigjiga University, EthiopiaAklilu Lemma Institute of Pathobiology (ALIPB), Addis Ababa University (AAU), P.O. Box 1176, Addis Ababa, EthiopiaAklilu Lemma Institute of Pathobiology (ALIPB), Addis Ababa University (AAU), P.O. Box 1176, Addis Ababa, Ethiopia; Department of Veterinary Medicine, College of Agriculture and Veterinary Medicine, United Arab Emirates University, PO Box 15551, Al Ain, United Arab EmiratesAklilu Lemma Institute of Pathobiology (ALIPB), Addis Ababa University (AAU), P.O. Box 1176, Addis Ababa, EthiopiaBackground: Refugees in developing countries have poor access to Tuberculosis (TB) care and control services. The understanding of genetic diversity and drug sensitivity patterns of M. tuberculosis (MTB) is important for the TB control program. However, there is no evidence that shows the drug sensitivity profiles and genetic diversity of MTB circulating among refugees residing in Ethiopia. This study aimed to investigate the genetic diversity of MTB strains and lineages, and to identify the drug sensitivity profiles of MTB isolated from refugees residing in Ethiopia. Methods: A cross-sectional study was conducted among 68 MTB positive cases isolated from presumptive TB refugees from February to August 2021. Data and samples were collected in the refugee camp clinics and both rapid TB Ag detection and region of difference (RD)-9 deletion typing were used to confirm the MTBs. Drug susceptibility test (DST) and molecular typing were done using Mycobacterium Growth Indicator Tube (MGIT) method and spoligotyping respectively. Results: DST and spoligotyping results were available for all 68 isolates. The isolates were grouped into 25 spoligotype patterns, which consisted of 1–31 isolates with 36.8% strain diversity. The international shared type (SIT)25 was predominant spoligotype pattern consisting of 31 (45.6%) isolates, followed by SIT24 comprising 5 (7.4%) isolates. Further investigation showed that 64.7% (44/68) of the isolates were belonged to CAS1-Delhi family and 75% (51/68) of the isolates were belonged to lineage(L)-3. Multi-drug resistance (MDR)-TB was observed only in one isolate (1.5%) for first-line anti-TB drugs and the highest level of mono-resistance, 5.9% (4/68), was observed for PZA(Pyrazinamide). Mono-resistance was observed in 2.9 % (2/68) and while 97.0% (66/68) of the MTB positive cases were susceptible to the second-line anti-TB drugs. Conclusion: The findings are useful evidence for the TB screening, treatment and control in refugee populations and surrounding communities in Ethiopia.http://www.sciencedirect.com/science/article/pii/S240557942300027XTuberculosisDrug susceptibility testingSpoligotypingM. tuberculosisRefugees |
| spellingShingle | Abyot Meaza Getu Diriba Musse Girma Ammanuel Wondimu Getnet Worku Girmay Medhin Gobena Ameni Balako Gumi Molecular typing and drug sensitivity profiles of M. Tuberculosis isolated from refugees residing in Ethiopia Journal of Clinical Tuberculosis and Other Mycobacterial Diseases Tuberculosis Drug susceptibility testing Spoligotyping M. tuberculosis Refugees |
| title | Molecular typing and drug sensitivity profiles of M. Tuberculosis isolated from refugees residing in Ethiopia |
| title_full | Molecular typing and drug sensitivity profiles of M. Tuberculosis isolated from refugees residing in Ethiopia |
| title_fullStr | Molecular typing and drug sensitivity profiles of M. Tuberculosis isolated from refugees residing in Ethiopia |
| title_full_unstemmed | Molecular typing and drug sensitivity profiles of M. Tuberculosis isolated from refugees residing in Ethiopia |
| title_short | Molecular typing and drug sensitivity profiles of M. Tuberculosis isolated from refugees residing in Ethiopia |
| title_sort | molecular typing and drug sensitivity profiles of m tuberculosis isolated from refugees residing in ethiopia |
| topic | Tuberculosis Drug susceptibility testing Spoligotyping M. tuberculosis Refugees |
| url | http://www.sciencedirect.com/science/article/pii/S240557942300027X |
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