Evidence of carbamate resistance in urban populations of <it>Anopheles gambiae s.s.</it> mosquitoes resistant to DDT and deltamethrin insecticides in Lagos, South-Western Nigeria
<p>Abstract</p> <p>Background</p> <p>Resistance monitoring is essential in ensuring the success of insecticide based vector control programmes. This study was carried out to assess the susceptibility status of urban populations of <it>Anopheles gambiae</it>...
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BMC
2012-06-01
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| Series: | Parasites & Vectors |
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| Online Access: | http://www.parasitesandvectors.com/content/5/1/116 |
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| author | Oduola Adedayo O Idowu Emmanuel T Oyebola Muyiwa K Adeogun Adedapo O Olojede Judith B Otubanjo Olubunmi A Awolola Taiwo S |
| author_facet | Oduola Adedayo O Idowu Emmanuel T Oyebola Muyiwa K Adeogun Adedapo O Olojede Judith B Otubanjo Olubunmi A Awolola Taiwo S |
| author_sort | Oduola Adedayo O |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Resistance monitoring is essential in ensuring the success of insecticide based vector control programmes. This study was carried out to assess the susceptibility status of urban populations of <it>Anopheles gambiae</it> to carbamate insecticide being considered for vector control in mosquito populations previously reported to be resistant to DDT and permethrin.</p> <p>Methods</p> <p>Two – three day old adult female <it>Anopheles</it> mosquitoes reared from larval collections in 11 study sites from Local Government Areas of Lagos were exposed to test papers impregnated with DDT 4%, deltamethrin 0.05% and propoxur 0.1% insecticides. Additional tests were carried out to determine the susceptibility status of the <it>Anopheles gambiae</it> population to bendiocarb insecticide. Members of the <it>A. gambiae complex</it>, the molecular forms, were identified by PCR assays. The involvement of metabolic enzymes in carbamate resistance was assessed using Piperonyl butoxide (PBO) synergist assays. The presence of kdr-<it>w/e</it> and ace-1R point mutations responsible for DDT-pyrethroid and carbamate resistance mechanisms was also investigated by PCR.</p> <p>Results</p> <p>Propoxur resistance was found in 10 out of the 11 study sites. Resistance to three classes of insecticides was observed in five urban localities. Mortality rates in mosquitoes exposed to deltamethrin and propoxur did not show any significant difference (P > 0.05) but was significantly higher (P < 0.05) in populations exposed to DDT. All mosquitoes tested were identified as <it>A. gambiae s.s</it> (M form). The <it>kdr -w</it> point mutation at allelic frequencies between 45%-77% was identified as one of the resistant mechanisms responsible for DDT and pyrethroid resistance. <it>Ace-1R</it> point mutation was absent in the carbamate resistant population. However, the possible involvement of metabolic resistance was confirmed by synergistic assays conducted.</p> <p>Conclusion</p> <p>Evidence of carbamate resistance in <it>A. gambiae</it> populations already harbouring resistance to DDT and permethrin is a clear indication that calls for the implementation of insecticide resistance management strategies to combat the multiple resistance identified.</p> |
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| issn | 1756-3305 |
| language | English |
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| spelling | doaj.art-0228008da705429f8a010c1e23288a792022-12-21T23:20:59ZengBMCParasites & Vectors1756-33052012-06-015111610.1186/1756-3305-5-116Evidence of carbamate resistance in urban populations of <it>Anopheles gambiae s.s.</it> mosquitoes resistant to DDT and deltamethrin insecticides in Lagos, South-Western NigeriaOduola Adedayo OIdowu Emmanuel TOyebola Muyiwa KAdeogun Adedapo OOlojede Judith BOtubanjo Olubunmi AAwolola Taiwo S<p>Abstract</p> <p>Background</p> <p>Resistance monitoring is essential in ensuring the success of insecticide based vector control programmes. This study was carried out to assess the susceptibility status of urban populations of <it>Anopheles gambiae</it> to carbamate insecticide being considered for vector control in mosquito populations previously reported to be resistant to DDT and permethrin.</p> <p>Methods</p> <p>Two – three day old adult female <it>Anopheles</it> mosquitoes reared from larval collections in 11 study sites from Local Government Areas of Lagos were exposed to test papers impregnated with DDT 4%, deltamethrin 0.05% and propoxur 0.1% insecticides. Additional tests were carried out to determine the susceptibility status of the <it>Anopheles gambiae</it> population to bendiocarb insecticide. Members of the <it>A. gambiae complex</it>, the molecular forms, were identified by PCR assays. The involvement of metabolic enzymes in carbamate resistance was assessed using Piperonyl butoxide (PBO) synergist assays. The presence of kdr-<it>w/e</it> and ace-1R point mutations responsible for DDT-pyrethroid and carbamate resistance mechanisms was also investigated by PCR.</p> <p>Results</p> <p>Propoxur resistance was found in 10 out of the 11 study sites. Resistance to three classes of insecticides was observed in five urban localities. Mortality rates in mosquitoes exposed to deltamethrin and propoxur did not show any significant difference (P > 0.05) but was significantly higher (P < 0.05) in populations exposed to DDT. All mosquitoes tested were identified as <it>A. gambiae s.s</it> (M form). The <it>kdr -w</it> point mutation at allelic frequencies between 45%-77% was identified as one of the resistant mechanisms responsible for DDT and pyrethroid resistance. <it>Ace-1R</it> point mutation was absent in the carbamate resistant population. However, the possible involvement of metabolic resistance was confirmed by synergistic assays conducted.</p> <p>Conclusion</p> <p>Evidence of carbamate resistance in <it>A. gambiae</it> populations already harbouring resistance to DDT and permethrin is a clear indication that calls for the implementation of insecticide resistance management strategies to combat the multiple resistance identified.</p>http://www.parasitesandvectors.com/content/5/1/116CarbamateDDTPyrethroidsInsecticide resistanceUrban<it>Anopheles gambiae</it> mosquitoesLagosNigeria |
| spellingShingle | Oduola Adedayo O Idowu Emmanuel T Oyebola Muyiwa K Adeogun Adedapo O Olojede Judith B Otubanjo Olubunmi A Awolola Taiwo S Evidence of carbamate resistance in urban populations of <it>Anopheles gambiae s.s.</it> mosquitoes resistant to DDT and deltamethrin insecticides in Lagos, South-Western Nigeria Parasites & Vectors Carbamate DDT Pyrethroids Insecticide resistance Urban <it>Anopheles gambiae</it> mosquitoes Lagos Nigeria |
| title | Evidence of carbamate resistance in urban populations of <it>Anopheles gambiae s.s.</it> mosquitoes resistant to DDT and deltamethrin insecticides in Lagos, South-Western Nigeria |
| title_full | Evidence of carbamate resistance in urban populations of <it>Anopheles gambiae s.s.</it> mosquitoes resistant to DDT and deltamethrin insecticides in Lagos, South-Western Nigeria |
| title_fullStr | Evidence of carbamate resistance in urban populations of <it>Anopheles gambiae s.s.</it> mosquitoes resistant to DDT and deltamethrin insecticides in Lagos, South-Western Nigeria |
| title_full_unstemmed | Evidence of carbamate resistance in urban populations of <it>Anopheles gambiae s.s.</it> mosquitoes resistant to DDT and deltamethrin insecticides in Lagos, South-Western Nigeria |
| title_short | Evidence of carbamate resistance in urban populations of <it>Anopheles gambiae s.s.</it> mosquitoes resistant to DDT and deltamethrin insecticides in Lagos, South-Western Nigeria |
| title_sort | evidence of carbamate resistance in urban populations of it anopheles gambiae s s it mosquitoes resistant to ddt and deltamethrin insecticides in lagos south western nigeria |
| topic | Carbamate DDT Pyrethroids Insecticide resistance Urban <it>Anopheles gambiae</it> mosquitoes Lagos Nigeria |
| url | http://www.parasitesandvectors.com/content/5/1/116 |
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