Investigation of <i>Morinda citrifolia</i> Activities through Pinoresinol and α-EG Related Gene Expression
α-EG is a unique substance that was first found in the leaves and fruits of <i>Morinda citrifolia</i> (Mc) growing in Thailand using GC-MS at 52.33% and 54.12%. It was then concentrated and its abundance quantified, along with that of pinoresinol, via GC, compared to the standards in lea...
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MDPI AG
2022-07-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/11/15/1985 |
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| author | Runglawan Sudmoon Sanit Kaewdaungdee Unchaleeporn Ameamsri Tawatchai Tanee Pornnarong Siripiyasing Warin Wonok Arunrat Chaveerach |
| author_facet | Runglawan Sudmoon Sanit Kaewdaungdee Unchaleeporn Ameamsri Tawatchai Tanee Pornnarong Siripiyasing Warin Wonok Arunrat Chaveerach |
| author_sort | Runglawan Sudmoon |
| collection | DOAJ |
| description | α-EG is a unique substance that was first found in the leaves and fruits of <i>Morinda citrifolia</i> (Mc) growing in Thailand using GC-MS at 52.33% and 54.12%. It was then concentrated and its abundance quantified, along with that of pinoresinol, via GC, compared to the standards in leaves, ufp, rfp, rawfs, and seeds. α-EG and pinoresinol, which have collagen stimulating, skin whitening, and an inhibitory effect on wrinkle formation, were found in different concentrations and amounts. Three different concentrations of the five Mc part extracts were tested on <i>NHDF</i> for gene expression related to the aforementioned activities, <i>COL1A1</i>, <i>COL1A2</i>, and <i>COL3A1</i>, <i>FGF1</i> and <i>FGF7</i> by qRT-PCR. The results showed various expression levels, both stimulatory and inhibitory, with different concentrations of plant parts and genes. Similar results were revealed when the experiments were performed with <i>Morus alba</i> (Ma), which was found to contain 20.48 g protein p/100 g leaves at concentrations of 3.11 mg/mL. The studied Mc parts seem to have advantages based on the stated objectives, gene type and level of activity of each plant part. Rawfs and leaves supplemented with Ma samples were selected for toxicity tests with PBMCs. The lack of both cell and DNA toxicity from the rawfs indicated that they can be used safely. |
| first_indexed | 2024-03-09T05:05:37Z |
| format | Article |
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| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-09T05:05:37Z |
| publishDate | 2022-07-01 |
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| record_format | Article |
| series | Plants |
| spelling | doaj.art-022b571fa0734a9d89027865b6adf68d2023-12-03T12:55:17ZengMDPI AGPlants2223-77472022-07-011115198510.3390/plants11151985Investigation of <i>Morinda citrifolia</i> Activities through Pinoresinol and α-EG Related Gene ExpressionRunglawan Sudmoon0Sanit Kaewdaungdee1Unchaleeporn Ameamsri2Tawatchai Tanee3Pornnarong Siripiyasing4Warin Wonok5Arunrat Chaveerach6Faculty of Law, Khon Kaen University, Khon Kaen 40002, ThailandDepartment of Biology, Faculty of Science, Khon Kaen University, Khon Kaen 40002, ThailandDepartment of Biology, Faculty of Science, Khon Kaen University, Khon Kaen 40002, ThailandFaculty of Environment and Resource Studies, Mahasarakham University, Maha Sarakham 44150, ThailandFaculty of Science and Technology, Rajabhat Mahasarakham University, Maha Sarakham 44150, ThailandDepartment of Biology, Faculty of Science, Khon Kaen University, Khon Kaen 40002, ThailandDepartment of Biology, Faculty of Science, Khon Kaen University, Khon Kaen 40002, Thailandα-EG is a unique substance that was first found in the leaves and fruits of <i>Morinda citrifolia</i> (Mc) growing in Thailand using GC-MS at 52.33% and 54.12%. It was then concentrated and its abundance quantified, along with that of pinoresinol, via GC, compared to the standards in leaves, ufp, rfp, rawfs, and seeds. α-EG and pinoresinol, which have collagen stimulating, skin whitening, and an inhibitory effect on wrinkle formation, were found in different concentrations and amounts. Three different concentrations of the five Mc part extracts were tested on <i>NHDF</i> for gene expression related to the aforementioned activities, <i>COL1A1</i>, <i>COL1A2</i>, and <i>COL3A1</i>, <i>FGF1</i> and <i>FGF7</i> by qRT-PCR. The results showed various expression levels, both stimulatory and inhibitory, with different concentrations of plant parts and genes. Similar results were revealed when the experiments were performed with <i>Morus alba</i> (Ma), which was found to contain 20.48 g protein p/100 g leaves at concentrations of 3.11 mg/mL. The studied Mc parts seem to have advantages based on the stated objectives, gene type and level of activity of each plant part. Rawfs and leaves supplemented with Ma samples were selected for toxicity tests with PBMCs. The lack of both cell and DNA toxicity from the rawfs indicated that they can be used safely.https://www.mdpi.com/2223-7747/11/15/1985α-EGpinoresinol<i>Morinda citrifolia</i><i>Morus alba</i>qRT-PCR<i>COL1A1</i> |
| spellingShingle | Runglawan Sudmoon Sanit Kaewdaungdee Unchaleeporn Ameamsri Tawatchai Tanee Pornnarong Siripiyasing Warin Wonok Arunrat Chaveerach Investigation of <i>Morinda citrifolia</i> Activities through Pinoresinol and α-EG Related Gene Expression Plants α-EG pinoresinol <i>Morinda citrifolia</i> <i>Morus alba</i> qRT-PCR <i>COL1A1</i> |
| title | Investigation of <i>Morinda citrifolia</i> Activities through Pinoresinol and α-EG Related Gene Expression |
| title_full | Investigation of <i>Morinda citrifolia</i> Activities through Pinoresinol and α-EG Related Gene Expression |
| title_fullStr | Investigation of <i>Morinda citrifolia</i> Activities through Pinoresinol and α-EG Related Gene Expression |
| title_full_unstemmed | Investigation of <i>Morinda citrifolia</i> Activities through Pinoresinol and α-EG Related Gene Expression |
| title_short | Investigation of <i>Morinda citrifolia</i> Activities through Pinoresinol and α-EG Related Gene Expression |
| title_sort | investigation of i morinda citrifolia i activities through pinoresinol and α eg related gene expression |
| topic | α-EG pinoresinol <i>Morinda citrifolia</i> <i>Morus alba</i> qRT-PCR <i>COL1A1</i> |
| url | https://www.mdpi.com/2223-7747/11/15/1985 |
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