A high-throughput protocol for measuring solution pH of bacterial cultures using UV-Vis absorption spectrophotometry

A high-throughput protocol for measuring solution pH of bacterial cultures using UV-Vis absorption spectrophotometry

Summary: We present a protocol for measuring the pH of cell-free bacterial-conditioned media based on changes in the ultraviolet-visible (UV-Vis) absorbance spectrum using the pH indicator dye litmus. This protocol includes detailed procedures for performing bacterial culturing, examining bacterial...

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Bibliographic Details
Main Authors:	Kristen A. Engevik, Hyland Gonzalez, Clay Daniels, Ronald K. Stavert, Numan Oezguen, Melinda A. Engevik, Thomas D. Horvath
Format:	Article
Language:	English
Published:	Elsevier 2023-09-01
Series:	STAR Protocols
Subjects:	High Throughput Screening Microbiology
Online Access:	http://www.sciencedirect.com/science/article/pii/S2666166723005075

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