Extraction Optimization, Characterization and Biological Activities of Polysaccharide Extracts from <i>Nymphaea hybrid</i>
In this study, polysaccharide–rich <i>Nymphaea hybrid</i> extracts (NHE) were obtained using the ultrasound-assisted cellulase extraction (UCE) method optimized by response surface methodology (RSM). The structural properties and thermal stability of NHE were characterized by Fourier-tra...
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MDPI AG
2023-05-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/10/8974 |
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| author | Hui-Min Liu Wei Tang Sheng-Nan Lei Yun Zhang Ming-Yan Cheng Qing-Lei Liu Wei Wang |
| author_facet | Hui-Min Liu Wei Tang Sheng-Nan Lei Yun Zhang Ming-Yan Cheng Qing-Lei Liu Wei Wang |
| author_sort | Hui-Min Liu |
| collection | DOAJ |
| description | In this study, polysaccharide–rich <i>Nymphaea hybrid</i> extracts (NHE) were obtained using the ultrasound-assisted cellulase extraction (UCE) method optimized by response surface methodology (RSM). The structural properties and thermal stability of NHE were characterized by Fourier-transform infrared (FT–IR), high–performance liquid chromatography (HPLC) and thermogravimetry–derivative thermogravimetry (TG–DTG) analysis, respectively. Moreover, the bioactivities of NHE, including the antioxidant, anti–inflammatory, whitening and scratch healing activities were evaluated by different in vitro assays. NHE conveyed a good ability to scavenge against the 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radicals and inhibit the hyaluronidase activity. NHE can effectively protect the HaCaT cells against oxidative damage by inhibiting the intracellular reactive oxygen species (ROS) production in the H<sub>2</sub>O<sub>2</sub> stimulation assays and promoting the proliferation and migration in the scratch assays. In addition, NHE was proven to inhibit melanin production in B16 cells. Collectively, the above results seem to be the evidence needed to promote the potential of NHE to be regarded as a new functional raw material in the cosmetics or food industries. |
| first_indexed | 2024-03-11T03:39:09Z |
| format | Article |
| id | doaj.art-022f179cffb941a3ad7e64b3a6387c13 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T03:39:09Z |
| publishDate | 2023-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-022f179cffb941a3ad7e64b3a6387c132023-11-18T01:45:13ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-05-012410897410.3390/ijms24108974Extraction Optimization, Characterization and Biological Activities of Polysaccharide Extracts from <i>Nymphaea hybrid</i>Hui-Min Liu0Wei Tang1Sheng-Nan Lei2Yun Zhang3Ming-Yan Cheng4Qing-Lei Liu5Wei Wang6School of Perfume & Aroma and Cosmetics, Shanghai Institute of Technology, Shanghai 201418, ChinaSchool of Perfume & Aroma and Cosmetics, Shanghai Institute of Technology, Shanghai 201418, ChinaSchool of Perfume & Aroma and Cosmetics, Shanghai Institute of Technology, Shanghai 201418, ChinaSchool of Perfume & Aroma and Cosmetics, Shanghai Institute of Technology, Shanghai 201418, ChinaSchool of Perfume & Aroma and Cosmetics, Shanghai Institute of Technology, Shanghai 201418, ChinaSchool of Perfume & Aroma and Cosmetics, Shanghai Institute of Technology, Shanghai 201418, ChinaSchool of Perfume & Aroma and Cosmetics, Shanghai Institute of Technology, Shanghai 201418, ChinaIn this study, polysaccharide–rich <i>Nymphaea hybrid</i> extracts (NHE) were obtained using the ultrasound-assisted cellulase extraction (UCE) method optimized by response surface methodology (RSM). The structural properties and thermal stability of NHE were characterized by Fourier-transform infrared (FT–IR), high–performance liquid chromatography (HPLC) and thermogravimetry–derivative thermogravimetry (TG–DTG) analysis, respectively. Moreover, the bioactivities of NHE, including the antioxidant, anti–inflammatory, whitening and scratch healing activities were evaluated by different in vitro assays. NHE conveyed a good ability to scavenge against the 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radicals and inhibit the hyaluronidase activity. NHE can effectively protect the HaCaT cells against oxidative damage by inhibiting the intracellular reactive oxygen species (ROS) production in the H<sub>2</sub>O<sub>2</sub> stimulation assays and promoting the proliferation and migration in the scratch assays. In addition, NHE was proven to inhibit melanin production in B16 cells. Collectively, the above results seem to be the evidence needed to promote the potential of NHE to be regarded as a new functional raw material in the cosmetics or food industries.https://www.mdpi.com/1422-0067/24/10/8974<i>Nymphaea hybrid</i>extractionantioxidantbioactivities |
| spellingShingle | Hui-Min Liu Wei Tang Sheng-Nan Lei Yun Zhang Ming-Yan Cheng Qing-Lei Liu Wei Wang Extraction Optimization, Characterization and Biological Activities of Polysaccharide Extracts from <i>Nymphaea hybrid</i> International Journal of Molecular Sciences <i>Nymphaea hybrid</i> extraction antioxidant bioactivities |
| title | Extraction Optimization, Characterization and Biological Activities of Polysaccharide Extracts from <i>Nymphaea hybrid</i> |
| title_full | Extraction Optimization, Characterization and Biological Activities of Polysaccharide Extracts from <i>Nymphaea hybrid</i> |
| title_fullStr | Extraction Optimization, Characterization and Biological Activities of Polysaccharide Extracts from <i>Nymphaea hybrid</i> |
| title_full_unstemmed | Extraction Optimization, Characterization and Biological Activities of Polysaccharide Extracts from <i>Nymphaea hybrid</i> |
| title_short | Extraction Optimization, Characterization and Biological Activities of Polysaccharide Extracts from <i>Nymphaea hybrid</i> |
| title_sort | extraction optimization characterization and biological activities of polysaccharide extracts from i nymphaea hybrid i |
| topic | <i>Nymphaea hybrid</i> extraction antioxidant bioactivities |
| url | https://www.mdpi.com/1422-0067/24/10/8974 |
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