Promoter regions of <it>Plasmodium vivax </it>are poorly or not recognized by <it>Plasmodium falciparum</it>
<p>Abstract</p> <p>Background</p> <p>Heterologous promoter analysis in <it>Plasmodium </it>has revealed the existence of conserved <it>cis </it>regulatory elements as promoters from different species can drive expression of reporter genes in hete...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2007-02-01
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| Series: | Malaria Journal |
| Online Access: | http://www.malariajournal.com/content/6/1/20 |
| _version_ | 1818756062213308416 |
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| author | del Portillo Hernando A Azevedo Mauro F |
| author_facet | del Portillo Hernando A Azevedo Mauro F |
| author_sort | del Portillo Hernando A |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Heterologous promoter analysis in <it>Plasmodium </it>has revealed the existence of conserved <it>cis </it>regulatory elements as promoters from different species can drive expression of reporter genes in heterologous transfection assays. Here, the functional characterization of different <it>Plasmodium vivax </it>promoters in <it>Plasmodium falciparum </it>using luciferase as the reporter gene is presented.</p> <p>Methods</p> <p>Luciferase reporter plasmids harboring the upstream regions of the <it>msp1</it>, <it>dhfr</it>, and <it>vir3 </it>genes as well as the full-length intergenic regions of the <it>vir23/24 </it>and <it>ef-1α </it>genes of <it>P. vivax </it>were constructed and transiently transfected in <it>P. falciparum</it>.</p> <p>Results</p> <p>Only the constructs with the full-length intergenic regions of the <it>vir23/24 </it>and <it>ef-1α </it>genes were recognized by the <it>P. falciparum </it>transcription machinery <it>albeit </it>to values approximately two orders of magnitude lower than those reported by <it>luc </it>plasmids harbouring promoter regions from <it>P. falciparum </it>and <it>Plasmodium berghei</it>. A bioinformatics approach allowed the identification of a motif (GCATAT) in the <it>ef-1α </it>intergenic region that is conserved in five <it>Plasmodium </it>species but is degenerate (GCANAN) in <it>P. vivax</it>. Mutations of this motif in the <it>P. berghei ef-1α </it>promoter region decreased reporter expression indicating it is active in gene expression in <it>Plasmodium</it>.</p> <p>Conclusion</p> <p>Together, this data indicates that promoter regions of <it>P. vivax </it>are poorly or not recognized by the <it>P. falciparum </it>transcription machinery suggesting the existence of <it>P. vivax</it>-specific transcription regulatory elements.</p> |
| first_indexed | 2024-12-18T05:49:04Z |
| format | Article |
| id | doaj.art-02327c58d22d48b9a6e729d6bbdba559 |
| institution | Directory Open Access Journal |
| issn | 1475-2875 |
| language | English |
| last_indexed | 2024-12-18T05:49:04Z |
| publishDate | 2007-02-01 |
| publisher | BMC |
| record_format | Article |
| series | Malaria Journal |
| spelling | doaj.art-02327c58d22d48b9a6e729d6bbdba5592022-12-21T21:18:58ZengBMCMalaria Journal1475-28752007-02-01612010.1186/1475-2875-6-20Promoter regions of <it>Plasmodium vivax </it>are poorly or not recognized by <it>Plasmodium falciparum</it>del Portillo Hernando AAzevedo Mauro F<p>Abstract</p> <p>Background</p> <p>Heterologous promoter analysis in <it>Plasmodium </it>has revealed the existence of conserved <it>cis </it>regulatory elements as promoters from different species can drive expression of reporter genes in heterologous transfection assays. Here, the functional characterization of different <it>Plasmodium vivax </it>promoters in <it>Plasmodium falciparum </it>using luciferase as the reporter gene is presented.</p> <p>Methods</p> <p>Luciferase reporter plasmids harboring the upstream regions of the <it>msp1</it>, <it>dhfr</it>, and <it>vir3 </it>genes as well as the full-length intergenic regions of the <it>vir23/24 </it>and <it>ef-1α </it>genes of <it>P. vivax </it>were constructed and transiently transfected in <it>P. falciparum</it>.</p> <p>Results</p> <p>Only the constructs with the full-length intergenic regions of the <it>vir23/24 </it>and <it>ef-1α </it>genes were recognized by the <it>P. falciparum </it>transcription machinery <it>albeit </it>to values approximately two orders of magnitude lower than those reported by <it>luc </it>plasmids harbouring promoter regions from <it>P. falciparum </it>and <it>Plasmodium berghei</it>. A bioinformatics approach allowed the identification of a motif (GCATAT) in the <it>ef-1α </it>intergenic region that is conserved in five <it>Plasmodium </it>species but is degenerate (GCANAN) in <it>P. vivax</it>. Mutations of this motif in the <it>P. berghei ef-1α </it>promoter region decreased reporter expression indicating it is active in gene expression in <it>Plasmodium</it>.</p> <p>Conclusion</p> <p>Together, this data indicates that promoter regions of <it>P. vivax </it>are poorly or not recognized by the <it>P. falciparum </it>transcription machinery suggesting the existence of <it>P. vivax</it>-specific transcription regulatory elements.</p>http://www.malariajournal.com/content/6/1/20 |
| spellingShingle | del Portillo Hernando A Azevedo Mauro F Promoter regions of <it>Plasmodium vivax </it>are poorly or not recognized by <it>Plasmodium falciparum</it> Malaria Journal |
| title | Promoter regions of <it>Plasmodium vivax </it>are poorly or not recognized by <it>Plasmodium falciparum</it> |
| title_full | Promoter regions of <it>Plasmodium vivax </it>are poorly or not recognized by <it>Plasmodium falciparum</it> |
| title_fullStr | Promoter regions of <it>Plasmodium vivax </it>are poorly or not recognized by <it>Plasmodium falciparum</it> |
| title_full_unstemmed | Promoter regions of <it>Plasmodium vivax </it>are poorly or not recognized by <it>Plasmodium falciparum</it> |
| title_short | Promoter regions of <it>Plasmodium vivax </it>are poorly or not recognized by <it>Plasmodium falciparum</it> |
| title_sort | promoter regions of it plasmodium vivax it are poorly or not recognized by it plasmodium falciparum it |
| url | http://www.malariajournal.com/content/6/1/20 |
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