Sodium caseinate hinders chymosin-induced aggregation of caseins in concentrated milk: The role of soluble caseins and calcium ions
Concentrated casein micelle suspensions show an altered balance between the colloidal and soluble phases compared with native skim milk. The objective of this research was to probe the role of such a change on the chymosin-driven destabilization of casein micelles. Skim milk was gently concentrated...
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Format: | Article |
Language: | English |
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Elsevier
2021-11-01
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Series: | JDS Communications |
Online Access: | http://www.sciencedirect.com/science/article/pii/S2666910221001538 |
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author | P. Krishnankutty Nair M. Corredig |
author_facet | P. Krishnankutty Nair M. Corredig |
author_sort | P. Krishnankutty Nair |
collection | DOAJ |
description | Concentrated casein micelle suspensions show an altered balance between the colloidal and soluble phases compared with native skim milk. The objective of this research was to probe the role of such a change on the chymosin-driven destabilization of casein micelles. Skim milk was gently concentrated by osmotic stressing to increase the volume fraction of the micelles while maintaining a constant ionic composition. In situ turbidity measurements demonstrated that the secondary stage of gelation was hindered in the concentrated suspensions. Addition of ionic calcium overcame this inhibition, whereas restoring the original concentration by redilution did not. This work clearly demonstrated that calcium plays a major role in decreasing electrostatic repulsion in casein micelles, but also showed the importance of noncolloidal proteins in altering the gelling functionality of casein micelles in concentrated milk. Additional calcium induces aggregation of these soluble caseins, restoring gelation in concentrates. |
first_indexed | 2024-03-13T01:35:29Z |
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id | doaj.art-02328e7788904bcf8ac198a3a48ce53b |
institution | Directory Open Access Journal |
issn | 2666-9102 |
language | English |
last_indexed | 2024-03-13T01:35:29Z |
publishDate | 2021-11-01 |
publisher | Elsevier |
record_format | Article |
series | JDS Communications |
spelling | doaj.art-02328e7788904bcf8ac198a3a48ce53b2023-07-04T05:10:42ZengElsevierJDS Communications2666-91022021-11-0126309312Sodium caseinate hinders chymosin-induced aggregation of caseins in concentrated milk: The role of soluble caseins and calcium ionsP. Krishnankutty Nair0M. Corredig1Corresponding author; Food Science Department, University of Guelph, Guelph, ON, Canada N1G 2W1Food Science Department, University of Guelph, Guelph, ON, Canada N1G 2W1Concentrated casein micelle suspensions show an altered balance between the colloidal and soluble phases compared with native skim milk. The objective of this research was to probe the role of such a change on the chymosin-driven destabilization of casein micelles. Skim milk was gently concentrated by osmotic stressing to increase the volume fraction of the micelles while maintaining a constant ionic composition. In situ turbidity measurements demonstrated that the secondary stage of gelation was hindered in the concentrated suspensions. Addition of ionic calcium overcame this inhibition, whereas restoring the original concentration by redilution did not. This work clearly demonstrated that calcium plays a major role in decreasing electrostatic repulsion in casein micelles, but also showed the importance of noncolloidal proteins in altering the gelling functionality of casein micelles in concentrated milk. Additional calcium induces aggregation of these soluble caseins, restoring gelation in concentrates.http://www.sciencedirect.com/science/article/pii/S2666910221001538 |
spellingShingle | P. Krishnankutty Nair M. Corredig Sodium caseinate hinders chymosin-induced aggregation of caseins in concentrated milk: The role of soluble caseins and calcium ions JDS Communications |
title | Sodium caseinate hinders chymosin-induced aggregation of caseins in concentrated milk: The role of soluble caseins and calcium ions |
title_full | Sodium caseinate hinders chymosin-induced aggregation of caseins in concentrated milk: The role of soluble caseins and calcium ions |
title_fullStr | Sodium caseinate hinders chymosin-induced aggregation of caseins in concentrated milk: The role of soluble caseins and calcium ions |
title_full_unstemmed | Sodium caseinate hinders chymosin-induced aggregation of caseins in concentrated milk: The role of soluble caseins and calcium ions |
title_short | Sodium caseinate hinders chymosin-induced aggregation of caseins in concentrated milk: The role of soluble caseins and calcium ions |
title_sort | sodium caseinate hinders chymosin induced aggregation of caseins in concentrated milk the role of soluble caseins and calcium ions |
url | http://www.sciencedirect.com/science/article/pii/S2666910221001538 |
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