Fish oil enhances recovery of intestinal microbiota and epithelial integrity in chronic rejection of intestinal transplant.

BACKGROUND: The intestinal chronic rejection (CR) is the major limitation to long-term survival of transplanted organs. This study aimed to investigate the interaction between intestinal microbiota and epithelial integrity in chronic rejection of intestinal transplantation, and to find out whether f...

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Main Authors: Qiurong Li, Qiang Zhang, Chenyang Wang, Chun Tang, Yanmei Zhang, Ning Li, Jieshou Li
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2011-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3117781?pdf=render
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author Qiurong Li
Qiang Zhang
Chenyang Wang
Chun Tang
Yanmei Zhang
Ning Li
Jieshou Li
author_facet Qiurong Li
Qiang Zhang
Chenyang Wang
Chun Tang
Yanmei Zhang
Ning Li
Jieshou Li
author_sort Qiurong Li
collection DOAJ
description BACKGROUND: The intestinal chronic rejection (CR) is the major limitation to long-term survival of transplanted organs. This study aimed to investigate the interaction between intestinal microbiota and epithelial integrity in chronic rejection of intestinal transplantation, and to find out whether fish oil enhances recovery of intestinal microbiota and epithelial integrity. METHODS/PRINCIPAL FINDINGS: The luminal and mucosal microbiota composition of CR rats were characterized by DGGE analysis at 190 days after intestinal transplant. The specific bacterial species were determined by sequence analysis. Furthermore, changes in the localization of intestinal TJ proteins were examined by immunofluorescent staining. PCR-DGGE analysis revealed that gut microbiota in CR rats had a shift towards Escherichia coli, Bacteroides spp and Clostridium spp and a decrease in the abundance of Lactobacillales bacteria in the intestines. Fish oil supplementation could enhance the recovery of gut microbiota, showing a significant decrease of gut bacterial proportions of E. coli and Bacteroides spp and an increase of Lactobacillales spp. In addition, CR rats showed pronounced alteration of tight junction, depicted by marked changes in epithelial cell ultrastructure and redistribution of occuldin and claudins as well as disruption in TJ barrier function. Fish oil administration ameliorated disruption of epithelial integrity in CR, which was associated with an improvement of the mucosal structure leading to improved tight junctions. CONCLUSIONS/SIGNIFICANCE: Our study have presented novel evidence that fish oil is involved in the maintenance of epithelial TJ integrity and recovery of gut microbiota, which may have therapeutic potential against CR in intestinal transplantation.
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spelling doaj.art-02353aef7f54494cb29d5b0d6db3584c2022-12-22T01:17:23ZengPublic Library of Science (PLoS)PLoS ONE1932-62032011-01-0166e2046010.1371/journal.pone.0020460Fish oil enhances recovery of intestinal microbiota and epithelial integrity in chronic rejection of intestinal transplant.Qiurong LiQiang ZhangChenyang WangChun TangYanmei ZhangNing LiJieshou LiBACKGROUND: The intestinal chronic rejection (CR) is the major limitation to long-term survival of transplanted organs. This study aimed to investigate the interaction between intestinal microbiota and epithelial integrity in chronic rejection of intestinal transplantation, and to find out whether fish oil enhances recovery of intestinal microbiota and epithelial integrity. METHODS/PRINCIPAL FINDINGS: The luminal and mucosal microbiota composition of CR rats were characterized by DGGE analysis at 190 days after intestinal transplant. The specific bacterial species were determined by sequence analysis. Furthermore, changes in the localization of intestinal TJ proteins were examined by immunofluorescent staining. PCR-DGGE analysis revealed that gut microbiota in CR rats had a shift towards Escherichia coli, Bacteroides spp and Clostridium spp and a decrease in the abundance of Lactobacillales bacteria in the intestines. Fish oil supplementation could enhance the recovery of gut microbiota, showing a significant decrease of gut bacterial proportions of E. coli and Bacteroides spp and an increase of Lactobacillales spp. In addition, CR rats showed pronounced alteration of tight junction, depicted by marked changes in epithelial cell ultrastructure and redistribution of occuldin and claudins as well as disruption in TJ barrier function. Fish oil administration ameliorated disruption of epithelial integrity in CR, which was associated with an improvement of the mucosal structure leading to improved tight junctions. CONCLUSIONS/SIGNIFICANCE: Our study have presented novel evidence that fish oil is involved in the maintenance of epithelial TJ integrity and recovery of gut microbiota, which may have therapeutic potential against CR in intestinal transplantation.http://europepmc.org/articles/PMC3117781?pdf=render
spellingShingle Qiurong Li
Qiang Zhang
Chenyang Wang
Chun Tang
Yanmei Zhang
Ning Li
Jieshou Li
Fish oil enhances recovery of intestinal microbiota and epithelial integrity in chronic rejection of intestinal transplant.
PLoS ONE
title Fish oil enhances recovery of intestinal microbiota and epithelial integrity in chronic rejection of intestinal transplant.
title_full Fish oil enhances recovery of intestinal microbiota and epithelial integrity in chronic rejection of intestinal transplant.
title_fullStr Fish oil enhances recovery of intestinal microbiota and epithelial integrity in chronic rejection of intestinal transplant.
title_full_unstemmed Fish oil enhances recovery of intestinal microbiota and epithelial integrity in chronic rejection of intestinal transplant.
title_short Fish oil enhances recovery of intestinal microbiota and epithelial integrity in chronic rejection of intestinal transplant.
title_sort fish oil enhances recovery of intestinal microbiota and epithelial integrity in chronic rejection of intestinal transplant
url http://europepmc.org/articles/PMC3117781?pdf=render
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