Chemical Composition and Biological Activity of Argentinian Propolis of Four Species of Stingless Bees
The chemical composition of propolis of four species of stingless bees (SLBs) from Argentina was determined, and its antibacterial and anticancer activity was evaluated on selected types of microbes and cancer cell lines. Volatile secretions of all propolis samples are formed by 174 C<sub>2<...
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MDPI AG
2022-11-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/27/22/7686 |
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| author | Valery A. Isidorov Jolanta Maslowiecka Lukasz Szoka Naldo Pellizzer Dora Miranda Ewa Olchowik-Grabarek Monika Zambrzycka Izabela Swiecicka |
| author_facet | Valery A. Isidorov Jolanta Maslowiecka Lukasz Szoka Naldo Pellizzer Dora Miranda Ewa Olchowik-Grabarek Monika Zambrzycka Izabela Swiecicka |
| author_sort | Valery A. Isidorov |
| collection | DOAJ |
| description | The chemical composition of propolis of four species of stingless bees (SLBs) from Argentina was determined, and its antibacterial and anticancer activity was evaluated on selected types of microbes and cancer cell lines. Volatile secretions of all propolis samples are formed by 174 C<sub>2</sub>–C<sub>15</sub> organic compounds, mainly mono- and sesquiterpenes and their derivatives. The chromatograms of ether extracts showed 287 peaks, of which 210 were identified. The most representative groups in the extracts of various propolis samples were diterpenoids (mainly resin acids), triterpenoids and phenolic compounds: long-chain alkenyl phenols, resorcinols and salicylates. The composition of both volatile and extractive compounds turned out to be species-specific; however, in both cases, the pairwise similarity of the propolis of <i>Scaptotrigona postica</i> and <i>Tetragonisca fiebrigi</i> versus that of <i>Tetragona clavipes</i> and <i>Melipona quadrifasciata quadrifasciata</i> was observed, which indicated the similarity of the preferences of the respective species when choosing plant sources of resin. The composition of the studied extracts completely lacked flavonoids and phenolcarboxylic acids, which are usually associated with the biological activity and medicinal properties of propolis. However, tests on selected microbial species and cancer cell lines showed such activity. All propolis samples tested against <i>Paenibacillus larvae</i>, two species of <i>Bacillus</i> and <i>E. coli</i> showed biofilm inhibition unrelated to the inhibition of bacterial growth, leading to a decrease in their pathogenicity. Testing the anticancer activity of ether extracts using five types of cell cultures showed that all four types of propolis studied inhibit the growth of cancer cells in a dose- and time-dependent manner. Propolis harvested by <i>T. clavipes</i> demonstrated the highest cytotoxicity on all tested cell lines. |
| first_indexed | 2024-03-09T18:07:33Z |
| format | Article |
| id | doaj.art-0237dc7b05f74886839ab15028476fb9 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T18:07:33Z |
| publishDate | 2022-11-01 |
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| series | Molecules |
| spelling | doaj.art-0237dc7b05f74886839ab15028476fb92023-11-24T09:19:26ZengMDPI AGMolecules1420-30492022-11-012722768610.3390/molecules27227686Chemical Composition and Biological Activity of Argentinian Propolis of Four Species of Stingless BeesValery A. Isidorov0Jolanta Maslowiecka1Lukasz Szoka2Naldo Pellizzer3Dora Miranda4Ewa Olchowik-Grabarek5Monika Zambrzycka6Izabela Swiecicka7Institute of Forest Sciences, Bialystok Technical University, 15-351 Bialystok, PolandInstitute of Forest Sciences, Bialystok Technical University, 15-351 Bialystok, PolandDepartment of Medicinal Chemistry, Medical University of Bialystok, 15-222 Bialystok, PolandFacultad de Ciencias Forestales, Universidad Nacional de Misiones, Bertoni 124, Eldorado 3380, Misiones, ArgentinaFacultad de Ciencias Forestales, Universidad Nacional de Misiones, Bertoni 124, Eldorado 3380, Misiones, ArgentinaDepartment of Microbiology, Biological Faculty, University of Bialystok, 15-328 Bialystok, PolandDepartment of Microbiology, Biological Faculty, University of Bialystok, 15-328 Bialystok, PolandDepartment of Microbiology, Biological Faculty, University of Bialystok, 15-328 Bialystok, PolandThe chemical composition of propolis of four species of stingless bees (SLBs) from Argentina was determined, and its antibacterial and anticancer activity was evaluated on selected types of microbes and cancer cell lines. Volatile secretions of all propolis samples are formed by 174 C<sub>2</sub>–C<sub>15</sub> organic compounds, mainly mono- and sesquiterpenes and their derivatives. The chromatograms of ether extracts showed 287 peaks, of which 210 were identified. The most representative groups in the extracts of various propolis samples were diterpenoids (mainly resin acids), triterpenoids and phenolic compounds: long-chain alkenyl phenols, resorcinols and salicylates. The composition of both volatile and extractive compounds turned out to be species-specific; however, in both cases, the pairwise similarity of the propolis of <i>Scaptotrigona postica</i> and <i>Tetragonisca fiebrigi</i> versus that of <i>Tetragona clavipes</i> and <i>Melipona quadrifasciata quadrifasciata</i> was observed, which indicated the similarity of the preferences of the respective species when choosing plant sources of resin. The composition of the studied extracts completely lacked flavonoids and phenolcarboxylic acids, which are usually associated with the biological activity and medicinal properties of propolis. However, tests on selected microbial species and cancer cell lines showed such activity. All propolis samples tested against <i>Paenibacillus larvae</i>, two species of <i>Bacillus</i> and <i>E. coli</i> showed biofilm inhibition unrelated to the inhibition of bacterial growth, leading to a decrease in their pathogenicity. Testing the anticancer activity of ether extracts using five types of cell cultures showed that all four types of propolis studied inhibit the growth of cancer cells in a dose- and time-dependent manner. Propolis harvested by <i>T. clavipes</i> demonstrated the highest cytotoxicity on all tested cell lines.https://www.mdpi.com/1420-3049/27/22/7686stingless beespropolischemical compositionplant sourcesanticancer activityantimicrobial activity |
| spellingShingle | Valery A. Isidorov Jolanta Maslowiecka Lukasz Szoka Naldo Pellizzer Dora Miranda Ewa Olchowik-Grabarek Monika Zambrzycka Izabela Swiecicka Chemical Composition and Biological Activity of Argentinian Propolis of Four Species of Stingless Bees Molecules stingless bees propolis chemical composition plant sources anticancer activity antimicrobial activity |
| title | Chemical Composition and Biological Activity of Argentinian Propolis of Four Species of Stingless Bees |
| title_full | Chemical Composition and Biological Activity of Argentinian Propolis of Four Species of Stingless Bees |
| title_fullStr | Chemical Composition and Biological Activity of Argentinian Propolis of Four Species of Stingless Bees |
| title_full_unstemmed | Chemical Composition and Biological Activity of Argentinian Propolis of Four Species of Stingless Bees |
| title_short | Chemical Composition and Biological Activity of Argentinian Propolis of Four Species of Stingless Bees |
| title_sort | chemical composition and biological activity of argentinian propolis of four species of stingless bees |
| topic | stingless bees propolis chemical composition plant sources anticancer activity antimicrobial activity |
| url | https://www.mdpi.com/1420-3049/27/22/7686 |
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